Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte4n17
(724 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 55 1e-08
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 38 0.001
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.072
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.67
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 2.1
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.7
SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating |S... 26 4.7
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 6.3
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 6.3
SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy... 25 8.3
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 25 8.3
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 54.8 bits (126), Expect = 1e-08
Identities = 33/111 (29%), Positives = 59/111 (53%)
Frame = +2
Query: 389 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 568
N+I F EL + C + + AE A +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 569 HSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFAT 721
S+I++N+ + I++ GN+ G +RK H+ F+ + + + P+F T
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL-----FDTERKHFKKGSDFPIFET 140
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 37.9 bits (84), Expect = 0.001
Identities = 35/130 (26%), Positives = 60/130 (46%)
Frame = +2
Query: 260 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 439
R ++G+VQ +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 440 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 619
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 620 VIGKHRKNHI 649
+I HRK H+
Sbjct: 148 LIAVHRKIHL 157
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.072
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -2
Query: 501 KVVVGPSSADSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 322
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 321 LTGR 310
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.67
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +2
Query: 473 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 583
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 27.5 bits (58), Expect = 2.1
Identities = 29/116 (25%), Positives = 48/116 (41%)
Frame = -1
Query: 454 RLLLPCAEREGHVPQLLETDDVNTLLAGNIDDFLDFIENCFLLLVDWTIGGHRDGMLNYS 275
R LL C +R P +L DV ++DD + F+ T GH++ + N S
Sbjct: 119 RALLSCCKRSKD-PSILFPTDVPC----SLDDDVSFL----------TFKGHKNHLENRS 163
Query: 274 YLHNSRGSGLLVLGRESVCGDVEVSLLSCSDRGFFQFDFKVIPPPKMNSVELFQVA 107
+ H+S V+ E + L DR K++ P N ++L +V+
Sbjct: 164 FFHDSESDNFKVVLSNCAINSKEDNNLVTEDR--VNLGAKLLLVPVQNLIKLLKVS 217
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.7
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -1
Query: 352 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRESVCGDVEVSL 197
+FI+ CF + + GH D +++ S +S GS +G S D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709
>SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 492
Score = 26.2 bits (55), Expect = 4.7
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -2
Query: 717 ANTGWPVLPSM**LDSLKSPTLGMWFLR-CFPITFPVSLITTAVFQSMSECFSSLSN 550
A+ G P++ + LD+ G W + + PVSLIT AVF + C SSL +
Sbjct: 247 ADDGKPLVEKI--LDAAGQKGTGKWTAQNALEMGTPVSLITEAVF---ARCLSSLKS 298
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 6.3
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 92 NNLTGRDLEEFNRIHFGRRNNLEI 163
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 6.3
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 62 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 172
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
>SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 922
Score = 25.4 bits (53), Expect = 8.3
Identities = 21/100 (21%), Positives = 38/100 (38%), Gaps = 2/100 (2%)
Frame = +2
Query: 407 ELWNMPFAFCTREKQPWCEFAESA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHSDI 580
+LWN C R + A S D + + ++ + SS++ER + H
Sbjct: 420 KLWNKKTTSCIRTIECGYVLAASFINNDKCIVSAYKSGELEVYDIASSSLIERIQAHDGA 479
Query: 581 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNT 700
+W+ AV G +H ++ S ++ G T
Sbjct: 480 IWDLAV--GHDGTYFATASADHTVKLWSLKSSFDFVPGTT 517
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 25.4 bits (53), Expect = 8.3
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -1
Query: 352 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRES 224
D N F+ ++ GH+D + + S GSG VLGR++
Sbjct: 353 DKSSNLFVTSKQSSVSGHKDNLTEEAPFSVSEGSG--VLGRQA 393
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,047,859
Number of Sequences: 5004
Number of extensions: 63523
Number of successful extensions: 221
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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