Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte4d05
(757 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 67 2e-12
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 41 2e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.008
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 33 0.058
SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p... 28 1.7
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 28 1.7
SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch... 27 2.2
SPAC12B10.07 |acp1||F-actin capping protein alpha subunit|Schizo... 27 2.2
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 26 5.0
SPCC330.19c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 6.7
SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch... 25 8.8
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 67.3 bits (157), Expect = 2e-12
Identities = 32/101 (31%), Positives = 59/101 (58%)
Frame = +1
Query: 349 QYPSYAEVMNEVERLKAGALRLLTREQVIYMLNKWVLEPDIKHELCLAFKVFDTEKRGFL 528
Q P+ AE+ + + + A + + + M+ + + + D + E+ AFKVFD + G++
Sbjct: 43 QSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYI 102
Query: 529 EIDELKTIVTSYAEPFSEEETREMLRDANVRGDGNVFYESF 651
++EL ++TS E S+EE +M+R+A+ GDG + YE F
Sbjct: 103 TVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 41.1 bits (92), Expect = 2e-04
Identities = 20/78 (25%), Positives = 42/78 (53%)
Frame = +1
Query: 436 YMLNKWVLEPDIKHELCLAFKVFDTEKRGFLEIDELKTIVTSYAEPFSEEETREMLRDAN 615
++ NK + E + + E AF+VFD + G++E + + + E S+ E + M+++A+
Sbjct: 66 FVSNK-LRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
Query: 616 VRGDGNVFYESFVESLFS 669
G+ Y FV+ + +
Sbjct: 125 PTNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.008
Identities = 24/80 (30%), Positives = 41/80 (51%), Gaps = 4/80 (5%)
Frame = +1
Query: 490 AFKVFDTEKRGFLEIDELKTIVTSYAEPFSEEETREMLRDANVRGDGNVFYESFVESLFS 669
AFK+FD++K ++ EL+ + + + E ++LRD + G G + E FV +
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 670 VAPE---LYEIKTDY-LYED 717
E L EIK + L++D
Sbjct: 102 KIVERDPLEEIKRAFELFDD 121
>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 786
Score = 32.7 bits (71), Expect = 0.058
Identities = 35/128 (27%), Positives = 50/128 (39%), Gaps = 7/128 (5%)
Frame = +1
Query: 331 FHRRNFQYPSYAEVMNEVERLKAGALRLLTREQVIYMLNKWVLEPDIKHELCLAFKVFDT 510
FHRR + ++ R K + + + Y KWVLE DI L L F V
Sbjct: 534 FHRRFLDAFFVVSLYKKLLRKKVSLADMESIDAEFYRSLKWVLENDITGILDLTFSV--- 590
Query: 511 EKRGFLEIDELKTIVTSYAEPFSEEETR-------EMLRDANVRGDGNVFYESFVESLFS 669
E+ F E+ ++ I +EE + E V N FY FVE
Sbjct: 591 EEDHFGEVRTVELITNGENIEVTEENKKKYVDLVTEWRVSKRVEQQFNAFYSGFVE---L 647
Query: 670 VAPELYEI 693
V+P+L +
Sbjct: 648 VSPDLVNV 655
>SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1098
Score = 27.9 bits (59), Expect = 1.7
Identities = 20/72 (27%), Positives = 34/72 (47%), Gaps = 3/72 (4%)
Frame = +3
Query: 39 INSNYFFDTMAVLDAYNPLQNVSLRFNEDQLKEIGEWFENNAKPQLT---STDGDPIRAI 209
I +NY F L L+ F + ++ GE+F+N K +LT +D + +R +
Sbjct: 461 ILNNYIFSCPLFLVTPEELEEQYTDFFKKLKEKYGEFFQNLLKKELTGKPESDLEGLRLV 520
Query: 210 TVDKFVSFLNLK 245
V ++ NLK
Sbjct: 521 GVQLQATYENLK 532
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 27.9 bits (59), Expect = 1.7
Identities = 16/81 (19%), Positives = 41/81 (50%), Gaps = 5/81 (6%)
Frame = +1
Query: 424 EQVIYMLNKWVLEPDIKHELCLAFKVFDTEKRGFLEIDELKTIV-----TSYAEPFSEEE 588
++ I L+ + + + + +L AFK++D ++ G++ EL ++ T+ E ++
Sbjct: 76 QEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQI 135
Query: 589 TREMLRDANVRGDGNVFYESF 651
+ + + + DG + +E F
Sbjct: 136 VDKTIMEVDKDRDGKISFEEF 156
>SPBC16E9.05 |erg6||delta-sterol C-methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 27.5 bits (58), Expect = 2.2
Identities = 17/54 (31%), Positives = 25/54 (46%)
Frame = +1
Query: 529 EIDELKTIVTSYAEPFSEEETREMLRDANVRGDGNVFYESFVESLFSVAPELYE 690
++DE + Y E + E D R DG Y+S V S + +A +LYE
Sbjct: 37 DVDEQSRKLQEYFEFWDRNHENESEEDRARRIDG---YKSVVNSYYDLATDLYE 87
>SPAC12B10.07 |acp1||F-actin capping protein alpha
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 256
Score = 27.5 bits (58), Expect = 2.2
Identities = 17/71 (23%), Positives = 33/71 (46%)
Frame = +1
Query: 535 DELKTIVTSYAEPFSEEETREMLRDANVRGDGNVFYESFVESLFSVAPELYEIKTDYLYE 714
++LK Y S + +++ A+ R +GN +Y+ ++ F++ E E + E
Sbjct: 38 EQLKLYHEDYNSSVSISDDEKVIISADNRLEGNRYYDQVLQKSFTINYETMEAEN---VE 94
Query: 715 DPNEDPSVPPE 747
D E +P E
Sbjct: 95 DYTEAIKIPDE 105
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +1
Query: 424 EQVIYMLNKWVLEPDIKHELCLAFKV 501
++V Y KW+ D+ LCL F V
Sbjct: 450 DEVYYESLKWIKNNDVDESLCLNFSV 475
>SPCC330.19c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 90
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -2
Query: 657 FNKTFIKYISIATDICIT*HFTCLLF 580
F TF+ IA CIT F CL+F
Sbjct: 52 FLHTFLADCEIAKSTCITSVFNCLIF 77
>SPAC1952.17c ||SPAC890.01c|GTPase activating
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 619
Score = 25.4 bits (53), Expect = 8.8
Identities = 9/44 (20%), Positives = 22/44 (50%)
Frame = +3
Query: 87 NPLQNVSLRFNEDQLKEIGEWFENNAKPQLTSTDGDPIRAITVD 218
N +N+S+ +D+++E+G+ + + P+ T+D
Sbjct: 147 NVSENISVNTEDDKVEEVGQKLNYTKITSIDQSQETPVHLSTID 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,850,537
Number of Sequences: 5004
Number of extensions: 56738
Number of successful extensions: 175
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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