Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte30i06
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 67 2e-12
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 48 2e-06
SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual 33 0.057
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.076
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.71
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 2.2
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.9
SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating |S... 26 5.0
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 26 5.0
SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase |Schiz... 26 5.0
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 6.6
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 6.6
SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy... 25 8.7
SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S... 25 8.7
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 25 8.7
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 67.3 bits (157), Expect = 2e-12
Identities = 38/122 (31%), Positives = 67/122 (54%)
Frame = +2
Query: 386 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 565
N+I F EL + C + + AE A +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 566 HSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNI 745
S+I++N+ + I++ GN+ G +RK H+ F+ + + + P+F T +GK+ V I
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL-----FDTERKHFKKGSDFPIFETSFGKLGVMI 149
Query: 746 CF 751
C+
Sbjct: 150 CW 151
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 47.6 bits (108), Expect = 2e-06
Identities = 47/170 (27%), Positives = 76/170 (44%), Gaps = 5/170 (2%)
Frame = +2
Query: 257 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 436
R ++G+VQ +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 437 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 616
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 617 VIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF 751
+I HRK H IP F ES+ G+ + T YGK + IC+
Sbjct: 148 LIAVHRKIHLFDIDIPGGVSFRESDSLSPGD-AMTMVDTEYGKFGLGICY 196
>SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 276
Score = 32.7 bits (71), Expect = 0.057
Identities = 35/140 (25%), Positives = 56/140 (40%), Gaps = 6/140 (4%)
Frame = +2
Query: 350 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAM 529
K++I A +G I F E + F E + +RE A K++
Sbjct: 23 KELISQAAAKGAKCIFFPEASD----FIAHNSDEAIELTNHPDCSKFIRDVRESATKHS- 77
Query: 530 VIVSSILERDEKHSDILWNTAVVISDT-GNVIGKHRKNHIPRVGDFN-----ESNYYMEG 691
+ V+ + K + L N+++ I G +I ++ K H+ V N ESN + G
Sbjct: 78 IFVNICVHEPSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKESNTTLRG 137
Query: 692 NTGHPVFATRYGKIAVNICF 751
P T GK+ ICF
Sbjct: 138 EAILPPCKTPLGKVGSAICF 157
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.076
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -2
Query: 498 KVVVGPSSADSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 319
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 318 LTGR 307
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.71
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +2
Query: 470 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 580
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 27.5 bits (58), Expect = 2.2
Identities = 29/116 (25%), Positives = 48/116 (41%)
Frame = -1
Query: 451 RLLLPCAEREGHVPQLLETDDVNTLLAGNIDDFLDFIENCFLLLVDWTIGGHRDGMLNYS 272
R LL C +R P +L DV ++DD + F+ T GH++ + N S
Sbjct: 119 RALLSCCKRSKD-PSILFPTDVPC----SLDDDVSFL----------TFKGHKNHLENRS 163
Query: 271 YLHNSRGSGLLVLGRESVCGDVEVSLLSCSDRGFFQFDFKVIPPPKMNSVELFQVA 104
+ H+S V+ E + L DR K++ P N ++L +V+
Sbjct: 164 FFHDSESDNFKVVLSNCAINSKEDNNLVTEDR--VNLGAKLLLVPVQNLIKLLKVS 217
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.9
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -1
Query: 349 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRESVCGDVEVSL 194
+FI+ CF + + GH D +++ S +S GS +G S D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709
>SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 492
Score = 26.2 bits (55), Expect = 5.0
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -2
Query: 714 ANTGWPVLPSM**LDSLKSPTLGMWFLR-CFPITFPVSLITTAVFQSMSECFSSLSN 547
A+ G P++ + LD+ G W + + PVSLIT AVF + C SSL +
Sbjct: 247 ADDGKPLVEKI--LDAAGQKGTGKWTAQNALEMGTPVSLITEAVF---ARCLSSLKS 298
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = -2
Query: 750 KQMFTAILPYLVANTGWPVLPS 685
K +FTA+ P+L A T + ++PS
Sbjct: 1529 KPVFTAVPPFLFAGTDFALIPS 1550
>SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/29 (44%), Positives = 14/29 (48%)
Frame = -1
Query: 739 HRDLAVSGRKYRMAGVTFHVVVGFVKIAD 653
HR+L V K A V H VVG K D
Sbjct: 202 HRELTVRAAKQHGARVLIHPVVGMTKPGD 230
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 89 NNLTGRDLEEFNRIHFGRRNNLEI 160
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 59 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 169
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
>SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 922
Score = 25.4 bits (53), Expect = 8.7
Identities = 21/100 (21%), Positives = 38/100 (38%), Gaps = 2/100 (2%)
Frame = +2
Query: 404 ELWNMPFAFCTREKQPWCEFAESA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHSDI 577
+LWN C R + A S D + + ++ + SS++ER + H
Sbjct: 420 KLWNKKTTSCIRTIECGYVLAASFINNDKCIVSAYKSGELEVYDIASSSLIERIQAHDGA 479
Query: 578 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNT 697
+W+ AV G +H ++ S ++ G T
Sbjct: 480 IWDLAV--GHDGTYFATASADHTVKLWSLKSSFDFVPGTT 517
>SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase
Mde10|Schizosaccharomyces pombe|chr 1|||Manual
Length = 512
Score = 25.4 bits (53), Expect = 8.7
Identities = 8/25 (32%), Positives = 14/25 (56%)
Frame = -3
Query: 743 CSPRSCRIWSQIQDGRCYLPCSSWI 669
C SC++ Q +DG C++ +I
Sbjct: 438 CHSDSCKVSCQNEDGTCFISAKDYI 462
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -1
Query: 349 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRES 221
D N F+ ++ GH+D + + S GSG VLGR++
Sbjct: 353 DKSSNLFVTSKQSSVSGHKDNLTEEAPFSVSEGSG--VLGRQA 393
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,177,065
Number of Sequences: 5004
Number of extensions: 66981
Number of successful extensions: 233
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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