Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte30h02
         (749 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1620.06c |||ribose-phosphate pyrophosphokinase |Schizosaccha...   367   e-103
SPBC3D6.06c |||ribose-phosphate pyrophosphokinase |Schizosacchar...   265   5e-72
SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizo...   243   2e-65
SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccha...    31   0.23 
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    29   0.71 
SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom...    28   1.6  
SPAC630.10 |||conserved fungal protein|Schizosaccharomyces pombe...    27   2.2  
SPBPJ4664.01 |dps1|SPBPJ694.01|decaprenyl diphosphate synthase s...    27   2.2  
SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce...    27   2.9  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   3.8  
SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom...    26   6.6  
SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces po...    26   6.6  
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   8.7  
SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit Git...    25   8.7  

>SPCC1620.06c |||ribose-phosphate pyrophosphokinase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 321

 Score =  367 bits (904), Expect = e-103
 Identities = 172/250 (68%), Positives = 216/250 (86%), Gaps = 1/250 (0%)
 Frame = +1

Query: 1   VFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIGESVRGEDVYIVQSGSGEIND 180
           +F+G+SHP+LA+K+  R+G+ LGKV   ++SN ET V IGESVR EDV+I+Q+G G IND
Sbjct: 8   IFAGNSHPELAEKVARRIGLSLGKVAVVQYSNRETSVTIGESVRDEDVFILQTGCGSIND 67

Query: 181 NLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPITAKLVANILSVSGADHII 360
           +LMELLIMINAC+ ASA R+TA+IPCFPYARQDKKDKSRAPITA+LVAN+L  +G +HII
Sbjct: 68  HLMELLIMINACRSASARRITAIIPCFPYARQDKKDKSRAPITARLVANMLQTAGCNHII 127

Query: 361 TMDLHASQIQGFFDIPVDNLFAEPAVLKWIKENIPDW-KTSIVVSPDAGGAKRVTSIADR 537
           TMDLHASQIQGFF++PVDNL+AEP+VL++I+ENI      +++VSPDAGGAKR T++ADR
Sbjct: 128 TMDLHASQIQGFFNVPVDNLYAEPSVLRYIRENIDTTVNPTVIVSPDAGGAKRATALADR 187

Query: 538 LNVEFALIHKERKKANEVASMVLVGDVKDRTAILVDDMADTCGTICHAAEKLIEAGATKV 717
           L+++FALIHKER+KANEV+ MVLVGDV+D+ AILVDDMADTCGT+  AA+ L + GA  V
Sbjct: 188 LDLDFALIHKERQKANEVSRMVLVGDVRDKLAILVDDMADTCGTLGLAAKTLKDNGAKAV 247

Query: 718 YAILTHGIFS 747
           YAI+THGI S
Sbjct: 248 YAIVTHGILS 257


>SPBC3D6.06c |||ribose-phosphate pyrophosphokinase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 341

 Score =  265 bits (649), Expect = 5e-72
 Identities = 143/273 (52%), Positives = 186/273 (68%), Gaps = 24/273 (8%)
 Frame = +1

Query: 1   VFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIGESVRGEDVYIVQSGSGEIND 180
           VF   SHP L + I + L +D+G+V   KFSN ET V I +SVRG DVYIV   SG++ND
Sbjct: 6   VFGTESHPKLTESICEHLCLDIGRVELSKFSNGETSVRIKQSVRGCDVYIVSPASGQVND 65

Query: 181 NLMELLIMINACKIASASRVTAVIPCFPYARQ-DKKDK-SRAPIT--------------- 309
           +LMELLIMI+ACK ASA +VTAV+P FPY+RQ D+K   S AP++               
Sbjct: 66  HLMELLIMISACKTASAKKVTAVLPVFPYSRQPDQKFSFSGAPLSDLQDAVVPCKKQTGY 125

Query: 310 -------AKLVANILSVSGADHIITMDLHASQIQGFFDIPVDNLFAEPAVLKWIKENIPD 468
                    LVA++L  SGADHIITMDLH  Q QGFFDIPVDNLF  P +  +I  NIP+
Sbjct: 126 HPWIAQSGTLVADLLMCSGADHIITMDLHDPQFQGFFDIPVDNLFGRPLLKHYISLNIPN 185

Query: 469 WKTSIVVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGDVKDRTAILVDD 648
           +  +++VSPDAGGAKR T+IAD L ++FALIHK R+      S++LVGDV+++ AIL+DD
Sbjct: 186 YHNAVIVSPDAGGAKRATAIADALGLDFALIHKNRRH-EYGTSLMLVGDVQNKVAILIDD 244

Query: 649 MADTCGTICHAAEKLIEAGATKVYAILTHGIFS 747
           + DT  T+  AAE + E GA+K+YA++TH + S
Sbjct: 245 LIDTAYTLVRAAEFVKEHGASKIYALVTHCVLS 277


>SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase
           Prs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score =  243 bits (595), Expect = 2e-65
 Identities = 113/195 (57%), Positives = 154/195 (78%)
 Frame = +1

Query: 1   VFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIGESVRGEDVYIVQSGSGEIND 180
           +  G SHP+L   I +RLGI    V  K+F+N ET VEI ESVR +DV+I+QSGS  +ND
Sbjct: 6   IIGGGSHPELLHLISNRLGITPCDVSLKRFANGETSVEIRESVRDKDVFILQSGSSTVND 65

Query: 181 NLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPITAKLVANILSVSGADHII 360
           +LMELLI+I+ACK  SA R+TAV+P FPY++Q K  K R  ITA++VAN+L+V+G DHII
Sbjct: 66  SLMELLIIISACKGGSAKRITAVMPYFPYSKQSKMRKYRDAITARMVANLLTVAGVDHII 125

Query: 361 TMDLHASQIQGFFDIPVDNLFAEPAVLKWIKENIPDWKTSIVVSPDAGGAKRVTSIADRL 540
           T+DLHASQ+QGFF  PVDNL+AEP + +WI+ N+ DW+ ++VVS + GGAKRVTS+AD L
Sbjct: 126 TLDLHASQMQGFFTRPVDNLYAEPNIAEWIRRNVDDWEEAVVVSKNPGGAKRVTSLADTL 185

Query: 541 NVEFALIHKERKKAN 585
           N++FALI+ +R++++
Sbjct: 186 NLDFALINTDRQRSS 200



 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 22/50 (44%), Positives = 31/50 (62%), Gaps = 1/50 (2%)
 Frame = +1

Query: 598 MVLVGDVKDRTAILVDDMADTCGTICHAAEKLIE-AGATKVYAILTHGIF 744
           + LVGDV  +TA+L+DD  +       A+E L++  GA +V  I THGIF
Sbjct: 294 ITLVGDVNGKTALLIDDTIENPTAFIVASEHLVKRCGAKRVIVIGTHGIF 343


>SPBC23E6.09 |ssn6||transcriptional corepressor
           Ssn6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1102

 Score = 30.7 bits (66), Expect = 0.23
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = +3

Query: 246 RHTLLPVRAARQKRQEQSPDHG--QTRCQHPFSVWSRSHHHYGSSCVANSRIL 398
           R TL P+ + +Q+  +QSP  G  Q   Q P SV   S  HY  + V   ++L
Sbjct: 59  RSTLHPLLSQQQQPAQQSPSLGPAQQNIQQPPSVSIASQPHYAEAIVPIQQVL 111


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 29.1 bits (62), Expect = 0.71
 Identities = 30/136 (22%), Positives = 56/136 (41%), Gaps = 4/136 (2%)
 Frame = +1

Query: 70  KVVTKKFSNMETCVEIGESV--RGEDVYIVQSGSGEINDNLMELLIMINACKIASASRVT 243
           +VVTK  S++  C+  G S+  R  D+ I +     +      L+I++      +    T
Sbjct: 120 QVVTKNTSHLYDCIRSGVSICNRLLDMEIFEPAISLLMKTHKNLIILLTYRDHDAIPTAT 179

Query: 244 AVIPCFPYARQDKKDKSRAP-ITAKLVANILSVSGADHI-ITMDLHASQIQGFFDIPVDN 417
            + P    +    +     P + A    NI ++     + +   L  SQI G     ++N
Sbjct: 180 LLNPTLDVSEIQLESCLFVPMVPASYFLNIGTIVVTFQLNVLRCLSLSQINGLSLNTINN 239

Query: 418 LFAEPAVLKWIKENIP 465
           L +E    +WI+ + P
Sbjct: 240 LQSEDGPFQWIERSFP 255


>SPCC1223.08c |dfr1||dihydrofolate reductase
           Dfr1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 461

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 19/74 (25%), Positives = 37/74 (50%), Gaps = 2/74 (2%)
 Frame = +1

Query: 352 HIITMDLHASQIQGFFDIPVDNLFA-EPAVLK-WIKENIPDWKTSIVVSPDAGGAKRVTS 525
           H +T +L  ++I    + P D+ F  EPA    W+++  P+ +  + +  + G  K  +S
Sbjct: 376 HPLTKNLLFTRIHK--EYPCDSFFPFEPAESSDWVRKAHPELEKFVGIPVEEGRLKAASS 433

Query: 526 IADRLNVEFALIHK 567
             + + +EF L  K
Sbjct: 434 NKEEVEIEFELYGK 447


>SPAC630.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = +1

Query: 253 PCFPYARQDKKDKSRAPITAKLVANILSVSGADHIITMDLHA 378
           PCF Y  + K+ K    I +  V N  S  G   +  +DLH+
Sbjct: 93  PCFSYCARLKEPKDLLEIGSVSVDNKCSTCGLFRVSRIDLHS 134


>SPBPJ4664.01 |dps1|SPBPJ694.01|decaprenyl diphosphate synthase
           subunit Dps1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 17/56 (30%), Positives = 28/56 (50%)
 Frame = +1

Query: 373 HASQIQGFFDIPVDNLFAEPAVLKWIKENIPDWKTSIVVSPDAGGAKRVTSIADRL 540
           H S IQ    + V+   A    + W KE I   K S++  PD+   K + ++AD++
Sbjct: 319 HPSDIQRARSL-VECTDAIEQTITWAKEYIKKAKDSLLCLPDSPARKALFALADKV 373


>SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 28/123 (22%), Positives = 55/123 (44%), Gaps = 16/123 (13%)
 Frame = +1

Query: 391 GFFDIPVDNLFAEPAVLK-WIKENIPDWKTSIVVSPDAGGAKRVTSIADRLNVEFAL--- 558
           GF    + N+F+ P  LK +++    D   SI    DA   + +   A++L V+  L   
Sbjct: 70  GFEVYTLRNIFSYPPELKGYMRTFGKDVDYSITTEQDAAMDQAIQEAAEKLVVKMQLRRD 129

Query: 559 ----IHKERKKANEVASMV----LVGDVKDRTAILV----DDMADTCGTICHAAEKLIEA 702
               + ++R+K  E+   +     +  V +   + +    D + D  G + HA ++++EA
Sbjct: 130 LRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDFLLDQLGNLQHAVKRVVEA 189

Query: 703 GAT 711
             T
Sbjct: 190 SPT 192


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
 Frame = +2

Query: 278 TKKTRAEPRSRPNSLPTSF-QCLEPITSSLWIFMRRKFKDSLIFR*ITSLP 427
           T+  R  PR      P ++   +EP TS +W+F   K K  L ++ +T +P
Sbjct: 76  TQYQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNK--LDYKAVTYVP 124


>SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 963

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 15/31 (48%), Positives = 18/31 (58%)
 Frame = -1

Query: 704 PASINFSAAWQIVPQVSAMSSTKMAVLSLTS 612
           PAS N S+   I PQVSA ++   A  S TS
Sbjct: 535 PASGNASSVSAIPPQVSAQTTIPAASTSATS 565


>SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 517

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 12/35 (34%), Positives = 17/35 (48%)
 Frame = +1

Query: 559 IHKERKKANEVASMVLVGDVKDRTAILVDDMADTC 663
           ++K+ KK N +    +V DVK R      D  D C
Sbjct: 1   MNKKDKKFNYIFGSEIVNDVKKRLPYYKSDWIDAC 35


>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 536

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = -1

Query: 656 SAMSSTKMAVLSLTSPTSTIEATSLAFFLSLWIKANSTFKRSAIDVTLLAPPASGDTT 483
           S+ SS+ ++  S TSP S+   TSL    S    ++S    S+   TL +  ++  T+
Sbjct: 59  SSSSSSPLSSSSFTSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTS 116


>SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit
           Git5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 305

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = +1

Query: 328 ILSVSGADHIITMDLHASQIQGFFDIP 408
           +LS S  D ++  DLH S    FF+ P
Sbjct: 36  LLSASTNDVLLVWDLHTSNKVAFFEAP 62


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,139,775
Number of Sequences: 5004
Number of extensions: 66422
Number of successful extensions: 227
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)