Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte30d24
         (712 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.06c |||leucine-rich repeat protein, unknown|Schizosaccha...    42   7e-05
SPBC1861.08c |||U2 snRNP-associated protein Lea1 |Schizosaccharo...    38   0.002
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22...    34   0.023
SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo...    33   0.053
SPBC32F12.08c |duo1||DASH complex subunit Duo1 |Schizosaccharomy...    29   0.50 
SPCC10H11.02 |cwf23|SPCP31B10.01|DNAJ domain protein Cwf23|Schiz...    27   2.6  
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po...    27   2.6  
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc...    27   3.5  
SPAC2F7.15 |rsm24||mitochondrial ribosomal protein subunit S24|S...    26   4.6  
SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharom...    26   4.6  
SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein Bms1|Schizosacc...    26   6.1  
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ...    25   8.1  
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    25   8.1  
SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces ...    25   8.1  

>SPAC926.06c |||leucine-rich repeat protein,
           unknown|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 621

 Score = 42.3 bits (95), Expect = 7e-05
 Identities = 22/53 (41%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
 Frame = +3

Query: 339 IENLERCESLRKLDLTLNFIGDIVSVETLMGNYSLESLYLTGNPCT-DYDNYK 494
           +  LE   SL KLD+  N I D+V    L+GN + E  YL+ NP T  Y +Y+
Sbjct: 418 LSGLENVPSLEKLDIRDNSITDVVEFRRLVGNTNFEEAYLSLNPFTKTYSSYR 470


>SPBC1861.08c |||U2 snRNP-associated protein Lea1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 239

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 20/70 (28%), Positives = 35/70 (50%)
 Frame = +3

Query: 363 SLRKLDLTLNFIGDIVSVETLMGNYSLESLYLTGNPCTDYDNYKNFVIGTLPQLTSLDGK 542
           +L+ L L  N + +I  ++ L     L +L    NP      Y+ ++I  +P L  LD +
Sbjct: 88  NLKTLSLAQNHLQEIADLDPLASCPQLTNLSCIDNPVAQKQYYRLYLIWRIPSLHILDFE 147

Query: 543 DIERSERIMA 572
            + R+ER+ A
Sbjct: 148 RVRRNERLRA 157


>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 332

 Score = 33.9 bits (74), Expect = 0.023
 Identities = 40/156 (25%), Positives = 70/156 (44%), Gaps = 4/156 (2%)
 Frame = +3

Query: 234 LKILLMQSNLIAKIEXXXXXXXXXXXXXXXXXIEVIENLERCESLRKLDLTLNFIGDIVS 413
           L+ L ++ N I KIE                 I  IENL+  ++L  LDL+ N I  I +
Sbjct: 64  LQSLCLRQNQIKKIESVPETLTELDLYDNL--IVRIENLDNVKNLTYLDLSFNNIKTIRN 121

Query: 414 VETLMGNYSLESLYLTGNPCTDYDNYKNFVIGTLPQLTSLD--GKDIERSERIMALQNLP 587
           +  L G   LE+L+   N     +N +      L +LT+L+  G  I   E +  L NL 
Sbjct: 122 INHLKG---LENLFFVQNRIRRIENLEG-----LDRLTNLELGGNKIRVIENLDTLVNLE 173

Query: 588 --FIRSDIVFEQENYRQQRRKQKSRLEKTIKDKWEN 689
             ++  + + + EN+ + ++     ++     ++EN
Sbjct: 174 KLWVGKNKITKFENFEKLQKLSLLSIQSNRITQFEN 209


>SPCC1739.11c |cdc11||SIN component scaffold protein
            Cdc11|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1045

 Score = 32.7 bits (71), Expect = 0.053
 Identities = 22/58 (37%), Positives = 31/58 (53%)
 Frame = +3

Query: 363  SLRKLDLTLNFIGDIVSVETLMGNYSLESLYLTGNPCTDYDNYKNFVIGTLPQLTSLD 536
            +LR LDL+ N+I DI S++ L     +  LYL GN      N  + ++  L QL  LD
Sbjct: 892  NLRVLDLSHNYISDIESLKPLQ---MIHRLYLVGNRIKKMRNLCD-ILANLKQLNVLD 945


>SPBC32F12.08c |duo1||DASH complex subunit Duo1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 166

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 25/103 (24%), Positives = 47/103 (45%), Gaps = 1/103 (0%)
 Frame = +3

Query: 405 IVSVETLMGNYSLESLYLTGNPCTDYDNYKNFVIGTLPQLTSLDGKDIERSERIMALQNL 584
           I S ET + N S   + L  +  +  ++ +N ++ +  +  S D +++ER +    LQ  
Sbjct: 33  IKSFETSINN-SNRLIQLWSSVLSQTEHTQNLILNSDWKGLSFDNEELERLQHQKMLQIQ 91

Query: 585 PFIRSDIVFEQENYR-QQRRKQKSRLEKTIKDKWENEYKDMDP 710
              +  I  +QE  R +Q R+QK       K + +   +  DP
Sbjct: 92  AEEQRKIELQQEQERLEQERRQKEEAIALQKQQQQRLLRSKDP 134


>SPCC10H11.02 |cwf23|SPCP31B10.01|DNAJ domain protein
           Cwf23|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 289

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 23/71 (32%), Positives = 41/71 (57%), Gaps = 13/71 (18%)
 Frame = +3

Query: 528 SLDGKDIERS---ERIMALQ----NLPFIRSDIVFEQENYRQQRRKQK------SRLEKT 668
           SL+ K+ ER    E++ ALQ    NL   R + + E++  + +RRKQ+      S L+++
Sbjct: 108 SLEKKENERDRLQEKLRALQEESANLRRQRENRLREEQE-QSKRRKQETPSSKISELDRS 166

Query: 669 IKDKWENEYKD 701
           I+ +W+ +Y D
Sbjct: 167 IRIRWKRKYAD 177


>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 897

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 10/63 (15%)
 Frame = -2

Query: 588 REGFVRP*SSRIFQYLCRPSWLAGVG-----FRLRSSCSYRNRCKGCQ-LNI----NFQD 439
           R GF+     R+ Q+ C P WL         FR+R S + RN CK    L I      QD
Sbjct: 545 RYGFLLVSLLRLRQFCCHP-WLVKSSSLDNSFRIRDSENVRNACKSLDPLTIERIATLQD 603

Query: 438 YSC 430
           ++C
Sbjct: 604 FNC 606


>SPAC27E2.06c |||methionine-tRNA ligase,
           mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 539

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 11/20 (55%), Positives = 16/20 (80%)
 Frame = -1

Query: 562 LSDLSISLPSKLVSWGRVPI 503
           +SDLSIS P + +SWG +P+
Sbjct: 225 ISDLSISRPKQRLSWG-IPV 243


>SPAC2F7.15 |rsm24||mitochondrial ribosomal protein subunit
           S24|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 258

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +1

Query: 619 KIIDSNVENKNLDWKKLSK 675
           K ID NV + NL W+K+ +
Sbjct: 61  KTIDDNVHSNNLSWQKIQE 79


>SPCP1E11.08 |||ribosome biogenesis protein Nsa2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 260

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 10/21 (47%), Positives = 17/21 (80%)
 Frame = +3

Query: 624 YRQQRRKQKSRLEKTIKDKWE 686
           Y+++RRK+K +++KTIK   E
Sbjct: 50  YQEKRRKEKIQMKKTIKQHEE 70


>SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein
           Bms1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1121

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 11/36 (30%), Positives = 21/36 (58%)
 Frame = +3

Query: 600 DIVFEQENYRQQRRKQKSRLEKTIKDKWENEYKDMD 707
           ++ F+ E     R+K++ RL    +D+ + E KD+D
Sbjct: 651 EVDFQTEREENARKKEELRLRFEEEDRGDPEKKDVD 686


>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 695

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 15/58 (25%), Positives = 31/58 (53%)
 Frame = +3

Query: 87  SL*KMVRITVELVRKKAEHHDCLLAPLEEIALHQENIEKIEFLQDWCPKLKILLMQSN 260
           +L +M ++   L+ K    +D  L  L+ + LH    +++E   ++  +LK  +MQ+N
Sbjct: 607 ALTEMEKVIKNLIDKSK--YDLALQSLQSLRLHSILEDEVERFNEYLTRLKKDVMQNN 662


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 7/22 (31%), Positives = 16/22 (72%)
 Frame = +3

Query: 624 YRQQRRKQKSRLEKTIKDKWEN 689
           +R+ +++   R+ KT++D WE+
Sbjct: 130 HRENKKEYVRRVRKTVEDSWES 151


>SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 905

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 22/104 (21%), Positives = 42/104 (40%), Gaps = 2/104 (1%)
 Frame = +3

Query: 378 DLTLNFIGDIVSVETLMGNYSLESLYLTGNPCTDYDNYKNFVIGTLPQLTSLDGKDIERS 557
           D     + D  S+    G+  L  +++     +D D+    ++ T+      DG+  ERS
Sbjct: 8   DKAAKTLSDAYSIAQSYGHSQLTPIHIAAALLSDSDSNGTTLLRTIVDKAGGDGQKFERS 67

Query: 558 --ERIMALQNLPFIRSDIVFEQENYRQQRRKQKSRLEKTIKDKW 683
              R++ L         +    E+ +  R   +  L+KT KD +
Sbjct: 68  VTSRLVRLPAQDPPPEQVTLSPESAKLLRNAHE--LQKTQKDSY 109


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,568,035
Number of Sequences: 5004
Number of extensions: 48512
Number of successful extensions: 181
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)