Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte30a03
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 29 0.52
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 29 0.69
SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 29 0.91
SPBC19G7.07c |||conserved fungal protein|Schizosaccharomyces pom... 28 1.2
SPAC343.14c |||translation initiation factor eIF2B|Schizosacchar... 27 2.1
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.8
SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 27 3.7
SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces ... 25 8.5
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 29.5 bits (63), Expect = 0.52
Identities = 29/99 (29%), Positives = 42/99 (42%), Gaps = 2/99 (2%)
Frame = +2
Query: 284 STQTGPSGL-VESLEMVRRGIILEQAANQRPLKGILNAASALDAFK*STRITKLKLTSRP 460
S+ T PS + S +V R A KG+ NAAS+ +F S I+K TSR
Sbjct: 589 SSPTKPSTTSLRSQSLVNRFASSTLKAPSSSSKGVSNAASSKQSFPSSPSISKKLETSRL 648
Query: 461 SIVTVP-SAIFATRFHRPTSTSAMVKSSASLASNITSCT 574
S +P S + A + +KS N+ + T
Sbjct: 649 STKKLPGSTMKAASALKEYPQQQSMKSGGEKQDNLVTIT 687
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 29.1 bits (62), Expect = 0.69
Identities = 15/55 (27%), Positives = 26/55 (47%), Gaps = 4/55 (7%)
Frame = +1
Query: 400 CSRCLQMINENNKIEIDKQAFHSHCAKCYFCHTV--PSSN--IHICYGQVFCEPC 552
C +C + + +++ + +HS C KC C+ PSS Q+FC+ C
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLC 78
>SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 649
Score = 28.7 bits (61), Expect = 0.91
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +1
Query: 616 EQFADPRFAENMKQFMAGKDGAP 684
E DP F EN KQ++ K G+P
Sbjct: 266 EYLNDPDFEENRKQYLQNKSGSP 288
>SPBC19G7.07c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 687
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +1
Query: 538 FCEPCFQHHVLHRHKDNPN 594
FC PC + LH HKDNPN
Sbjct: 17 FCGPCRR---LHYHKDNPN 32
>SPAC343.14c |||translation initiation factor
eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 393
Score = 27.5 bits (58), Expect = 2.1
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1
Query: 22 HTYPKKSSLIMDKGQEQVKKPF 87
HTYP SSLI D +V+ PF
Sbjct: 8 HTYPAVSSLIADLKSRKVQGPF 29
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.1 bits (57), Expect = 2.8
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Frame = +2
Query: 422 STRITKLKLTSRPSIVTVP--SAIFATRFHRPTSTSAMVKSSASLASNITSCTDIRTTP 592
ST I ++ P TVP S + P STS+ SS+ L S TSCT + P
Sbjct: 288 STSIPPTGNSTTPVTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIP 346
Score = 27.1 bits (57), Expect = 2.8
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Frame = +2
Query: 422 STRITKLKLTSRPSIVTVP--SAIFATRFHRPTSTSAMVKSSASLASNITSCTDIRTTP 592
ST I ++ P TVP S + P STS+ SS+ L S TSCT + P
Sbjct: 342 STSIPPTGNSTTPVTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIP 400
Score = 26.2 bits (55), Expect = 4.9
Identities = 15/45 (33%), Positives = 20/45 (44%)
Frame = +2
Query: 509 PTSTSAMVKSSASLASNITSCTDIRTTPMXXXXXXXXXXRTPDLP 643
PTSTS+ +S L + TSCT + P TP +P
Sbjct: 152 PTSTSSTDTNSNPLPTTSTSCTTSTSIPPTGGSSSLSTPITPTVP 196
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2
Query: 509 PTSTSAMVKSSASLASNITSCTDIRTTP 592
PTSTS+ +S+ L + TSCT + P
Sbjct: 209 PTSTSSTDTNSSPLPTTSTSCTTSTSIP 236
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2
Query: 509 PTSTSAMVKSSASLASNITSCTDIRTTP 592
PTSTS+ +S+ L + TSCT + P
Sbjct: 265 PTSTSSTDTNSSPLPTTSTSCTTSTSIP 292
>SPAC4H3.11c |ppc89|mug127|spindle pole body protein
Ppc89|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 26.6 bits (56), Expect = 3.7
Identities = 12/40 (30%), Positives = 24/40 (60%)
Frame = +1
Query: 340 DNPGASSKSEASEGNTKRSVCSRCLQMINENNKIEIDKQA 459
D+P +S E+ EG+ + S+ S +++++ E +KQA
Sbjct: 557 DSPNVASGVESDEGSEEISLDSEYSDILSDDGDFEKEKQA 596
>SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 557
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +1
Query: 325 NGQEGDNPGASSKSEASEGNTKRSVCSRCLQMINENN 435
+G + D+ ASS+S AS G RSV R + E++
Sbjct: 517 DGADNDDSSASSESFASAGAHSRSVFPRRSNVSEESH 553
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,238,397
Number of Sequences: 5004
Number of extensions: 68521
Number of successful extensions: 214
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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