Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte28n07
(579 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 181 6e-47
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc... 142 4e-35
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 48 1e-06
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 33 0.040
SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyce... 26 3.5
SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 25 8.0
SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces pom... 25 8.0
>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 181 bits (441), Expect = 6e-47
Identities = 74/130 (56%), Positives = 98/130 (75%)
Frame = +2
Query: 185 QQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKERPDLPPIQYEPVLCTRNTCRA 364
++ E+RDG+RFTWNV+PS+RIE++R +VP+A +Y+PL ERPDLPP+ YEPV C + C+A
Sbjct: 4 EEIEERDGVRFTWNVFPSTRIESSRTIVPIASIYKPLNERPDLPPVLYEPVTC-KAPCKA 62
Query: 365 ILNPMCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMP 544
+LNP C +D RAK W+C FC QRN PPQY IS P EL+P +STIEYT+ R +
Sbjct: 63 VLNPYCHIDTRAKFWICPFCLQRNMLPPQYKDISNTSLPIELLPEYSTIEYTLPRPPQLT 122
Query: 545 PIYLLVVDTC 574
P++L VVD C
Sbjct: 123 PVFLFVVDVC 132
>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 765
Score = 142 bits (343), Expect = 4e-35
Identities = 60/128 (46%), Positives = 84/128 (65%)
Frame = +2
Query: 194 EDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKERPDLPPIQYEPVLCTRNTCRAILN 373
ED+DGIR +WN + ++ E R V+P+A +Y PL E + QY+PV C R CRA+LN
Sbjct: 7 EDQDGIRLSWNTFSATPAENARAVIPIAAMYTPLHENERMTIEQYDPVAC-RAPCRAVLN 65
Query: 374 PMCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMPPIY 553
P C VD RA+ W+C FCFQRNP P QY+ IS P EL+ +T+EY +++ PP++
Sbjct: 66 PYCHVDLRARFWICPFCFQRNPLPAQYSDISSNSLPLELLSQSTTMEYVLSKPVKSPPVF 125
Query: 554 LLVVDTCL 577
L V+DT +
Sbjct: 126 LFVMDTAV 133
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 48.0 bits (109), Expect = 1e-06
Identities = 37/133 (27%), Positives = 51/133 (38%), Gaps = 11/133 (8%)
Frame = +2
Query: 158 DMATYEEFIQQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKE-RPDLPPIQY-- 328
D YE Q N ++FT P++ +PL + QP E RPD P+
Sbjct: 151 DFVGYE---QGNSSSKFVQFTSYAIPATNDVCNASGIPLGMIVQPFAELRPDEAPVPVVD 207
Query: 329 ----EPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQRNPFPPQ--YAAISEQHQPAEL 490
P C + CR +NP Q + W CN C N F + QH E
Sbjct: 208 NTNSNPPRCKK--CRGYINPFIQFTMASSKWTCNLCGSENSFNDDGFNPLVGSQHDGVES 265
Query: 491 IP--NFSTIEYTI 523
P N TI++ +
Sbjct: 266 RPELNVGTIDFKV 278
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 32.7 bits (71), Expect = 0.040
Identities = 21/85 (24%), Positives = 34/85 (40%), Gaps = 3/85 (3%)
Frame = +2
Query: 209 IRFTWNVWPSSRIEATRLVVPLACLYQP---LKERPDLPPIQYEPVLCTRNTCRAILNPM 379
+R T N P++ + +P A + +P L E D P+ + ++ CR +NP
Sbjct: 207 VRSTINCVPTTNSLLKKSKIPFALVIRPYTSLVEEDDPVPVVTDTIISRCRRCRMYINPF 266
Query: 380 CQVDYRAKLWVCNFCFQRNPFPPQY 454
+ CN C N P Y
Sbjct: 267 SIFIDNGHRYRCNSCGIVNEVPQSY 291
>SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 551
Score = 26.2 bits (55), Expect = 3.5
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = +2
Query: 437 PFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMPPIYLLVVDTC 574
P+ P Y ++S Q P+ + + YTI R +A+P + C
Sbjct: 2 PYNPLYESLSNQFDPSRIETVLDPMGYTIKR-RALPVSLTIAGSDC 46
>SPBC13E7.06 |msd1|mug172|spindle pole body protein
Msd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 331
Score = 25.0 bits (52), Expect = 8.0
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -1
Query: 183 MNSSYVAISPIKKSCGEPVINT*ANFLNHN 94
+NS + PIK++ P++ A+FL N
Sbjct: 171 LNSMLIVKEPIKQNTNAPILPETASFLQQN 200
>SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 373
Score = 25.0 bits (52), Expect = 8.0
Identities = 24/99 (24%), Positives = 41/99 (41%), Gaps = 2/99 (2%)
Frame = -3
Query: 343 TEY-RLVLYRW*VGALLKRLVQASQRHNKPRSLNST*WPYVPSEPYAIAVFILLDELLVC 167
T Y R++ W GA+L + + ++ + N + + PYAI L E
Sbjct: 150 TSYSRVIADLWLAGAILIYVTDSPNNSHRKQYENEVIMSMIAAYPYAIRFRQCLIERSSA 209
Query: 166 GHIADQKVMRRTSYKHMSKFLKSQY-VFIQKSHKFLNFL 53
+ +D+ S K+ + F +F +K FL FL
Sbjct: 210 DNSSDKFWSTLNSIKYFTAFPAIFLGIFAKKRFSFLWFL 248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,664,326
Number of Sequences: 5004
Number of extensions: 58508
Number of successful extensions: 154
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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