Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte28j24
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 43 4e-05
SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces ... 39 6e-04
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 39 6e-04
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 38 0.001
SPAPYUK71.03c |||C2 domain protein|Schizosaccharomyces pombe|chr... 34 0.017
SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom... 30 0.36
SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr 2|... 28 1.1
SPBC609.02 |ptn1||phosphatidylinositol-3,4,5-trisphosphate3-phos... 25 7.9
>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 786
Score = 43.2 bits (97), Expect = 4e-05
Identities = 20/68 (29%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +1
Query: 406 VESANIKNSGLFK-PNPYLQVIIDDKILRKTEVIRNTLHPKWKEDLTILVTPQSQLLFRL 582
V + + LF+ P+P+ + +D + T+VI+ +++P W E + V P S + RL
Sbjct: 13 VAADGLSKRDLFRQPDPFAILTVDGEQTHTTKVIKKSVNPYWNEGFEVTVKPSSVISIRL 72
Query: 583 ADYHSFRK 606
D F+K
Sbjct: 73 FDQKKFKK 80
>SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1429
Score = 39.1 bits (87), Expect = 6e-04
Identities = 18/71 (25%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Frame = +1
Query: 442 KPNPYLQVIIDDKILRKTEVIRNTLHPKWKEDLTILVTPQSQLL-FRLADYHSFRKDNVI 618
K +PY +VI+ + ++ +T N L+P W E L + + ++ + DY D +
Sbjct: 778 KSDPYARVIVGNSVVARTVYTPNNLNPIWNEILYVPIMADTKTIDLEAMDYEESGNDRSL 837
Query: 619 GEKKVNLLKVL 651
G +N+ K +
Sbjct: 838 GYASINVQKYI 848
Score = 33.5 bits (73), Expect = 0.030
Identities = 18/82 (21%), Positives = 42/82 (51%), Gaps = 2/82 (2%)
Frame = +1
Query: 409 ESANIKNSGLFKPNPYLQVIIDDKILRKTEVIRNTLHPKWKEDLTILVTPQSQLLFRLA- 585
+ N+ + +P++ + ++ + K++VI+ L+P W E+ I+V + + L
Sbjct: 1086 DGQNLPIGDIRSSDPFVVLKLNGESAFKSKVIKKNLNPVWNEEADIVVQNRVLDVLELVC 1145
Query: 586 -DYHSFRKDNVIGEKKVNLLKV 648
D+ K +V+G ++LL +
Sbjct: 1146 YDWDMGEKPDVLGTSNIDLLSL 1167
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 39.1 bits (87), Expect = 6e-04
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = +1
Query: 445 PNPYLQVIIDDKILRKTEVIRNTLHPKWKEDLTILVTPQSQLLFRLADYHSFRK 606
P+P+ + +D + T I+ TL+P W E + VT S + ++ D F+K
Sbjct: 29 PDPFAVLTVDGEQTHTTTAIKKTLNPYWNETFEVNVTDNSTIAIQVFDQKKFKK 82
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 37.9 bits (84), Expect = 0.001
Identities = 21/55 (38%), Positives = 27/55 (49%)
Frame = +1
Query: 424 KNSGLFKPNPYLQVIIDDKILRKTEVIRNTLHPKWKEDLTILVTPQSQLLFRLAD 588
KN L PYL + +DD KT V TL W + V+PQS +L +L D
Sbjct: 20 KNGLLRSLKPYLLISVDDDQFIKTNVASGTLRLSWGFTQKLTVSPQSIILLQLFD 74
>SPAPYUK71.03c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1225
Score = 34.3 bits (75), Expect = 0.017
Identities = 26/103 (25%), Positives = 50/103 (48%), Gaps = 3/103 (2%)
Frame = +1
Query: 340 NENISS--LTAPISVSTYHQLSLTVESANIKNSGLFKPNPYLQVIIDDKILRKTEVIRNT 513
N++ISS L PI H S + I K + Y +++ + +T VI +
Sbjct: 691 NDSISSMALATPIGAIRIHLRSANNLHSKIPGK---KCDSYARIMSHNTKQFRTVVIASN 747
Query: 514 LHPKWKEDLTILVTPQSQLLF-RLADYHSFRKDNVIGEKKVNL 639
++P W E + V + + F ++ +Y+S +D +IG+ +N+
Sbjct: 748 VNPFWDEYMYAPVITKHDIFFLQVMNYNSSGEDKLIGQTPINI 790
>SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1188
Score = 29.9 bits (64), Expect = 0.36
Identities = 22/69 (31%), Positives = 32/69 (46%), Gaps = 5/69 (7%)
Frame = +1
Query: 478 KILRKTEVIRNTLHPKWKEDLTILVTPQ-----SQLLFRLADYHSFRKDNVIGEKKVNLL 642
K L T V++ L+P W E I V P ++ L D F D+V+G K+ L
Sbjct: 503 KPLYCTRVVKQDLNPIWNEYAFIPVFPDQVKAGEKISIELWDSDRFSPDDVVGRTKIG-L 561
Query: 643 KVLFYCNGK 669
+L +GK
Sbjct: 562 HLLIQDSGK 570
>SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 272
Score = 28.3 bits (60), Expect = 1.1
Identities = 19/84 (22%), Positives = 39/84 (46%), Gaps = 3/84 (3%)
Frame = +1
Query: 409 ESANIKNSGLF-KPNPYLQVIIDDKILRKTEVIRNTLHPKWKEDLTILVTPQSQLLFRLA 585
++ N+ N L K +PY + + + R R+ P W L + +S + ++
Sbjct: 15 KAKNLPNKALVGKQSPYCVCRVGEVVKRTQTDKRSGQEPSWNAVLEFNIPSESYHIMKIT 74
Query: 586 DYH-SFRK-DNVIGEKKVNLLKVL 651
+H FRK ++IG+ ++ K +
Sbjct: 75 VFHEGFRKHPHLIGDTVLSFEKAM 98
>SPBC609.02 |ptn1||phosphatidylinositol-3,4,
5-trisphosphate3-phosphatase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 348
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/50 (26%), Positives = 22/50 (44%)
Frame = +3
Query: 300 MLHSKGLTKSIHYE*KYIFTHCSNFCVHISSTELNSGISKYKKFRLVQTK 449
M+ GLT S Y F ++ + E N+G + +K F+ + K
Sbjct: 164 MVRGHGLTISSQIRYVYYIEILKQFPNYLKAVEFNTGTTFFKSFKCLNIK 213
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,776,488
Number of Sequences: 5004
Number of extensions: 57086
Number of successful extensions: 124
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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