Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte28c09
(721 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 233 1e-62
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 233 1e-62
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 133 2e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 95 7e-21
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.51
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 27 2.0
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 27 2.7
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 27 3.6
SPCC1902.02 |mug72|SPCC663.16c|ketopantoate reductase |Schizosac... 26 6.2
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 26 6.2
SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|... 26 6.2
SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps90... 26 6.2
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 233 bits (571), Expect = 1e-62
Identities = 103/214 (48%), Positives = 147/214 (68%), Gaps = 8/214 (3%)
Frame = +1
Query: 103 MKEILHIHIGQAGVQAANACWELYCLEHGIRPDGVLA-----FPDN---DNSCGHFFSET 258
M+E++ +H+GQAGVQ NACWELYCLEHGI PDG +N ++ G FFSET
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 259 GAGKFVPRVVMIDLEPTPIDEIRTGVYRQLFHSSSLITGKEDAASNFARGYFGVGREMLD 438
G GKFVPR + +DLEP ID++RTG Y+ LFH ++TGKEDA++N+ARG++ VG+EM+D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 439 VALNRIRSVTEDCDNIQGFIVFRXXXXXXXXXXXXXXLESISRDFGKLSKIEYAIYPAPK 618
L RIR + ++C +QGF+VF LE ++ ++GK S +++++YPAP+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 619 ISPIIVESYNAVLTSHACMNTEDVCFLFDNEALY 720
+S +VE YN+VLT+HA ++ D F+ DNEA Y
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACY 214
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 233 bits (571), Expect = 1e-62
Identities = 101/210 (48%), Positives = 147/210 (70%), Gaps = 4/210 (1%)
Frame = +1
Query: 103 MKEILHIHIGQAGVQAANACWELYCLEHGIRPDGVL----AFPDNDNSCGHFFSETGAGK 270
M+EI+ IH+GQAG Q NACWELYCLEHGI+P+G + A ++D FFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 271 FVPRVVMIDLEPTPIDEIRTGVYRQLFHSSSLITGKEDAASNFARGYFGVGREMLDVALN 450
+VPR + +DLEP ID++RTG YR LFH LITGKEDA++N+ARG++ VG+E++D +
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 451 RIRSVTEDCDNIQGFIVFRXXXXXXXXXXXXXXLESISRDFGKLSKIEYAIYPAPKISPI 630
+IR + ++C +QGF+VF LE ++ ++ K SK+++++YPAP++S
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 631 IVESYNAVLTSHACMNTEDVCFLFDNEALY 720
+VE YN+VLT+HA ++ D F+ DNE+ Y
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCY 210
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 133 bits (322), Expect = 2e-32
Identities = 68/207 (32%), Positives = 103/207 (49%), Gaps = 2/207 (0%)
Frame = +1
Query: 103 MKEILHIHIGQAGVQAANACWELYCLEHGIRPDGVL--AFPDNDNSCGHFFSETGAGKFV 276
M+EI+HI GQ G Q A W EHG+ G+ +F+E GK+V
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 277 PRVVMIDLEPTPIDEIRTGVYRQLFHSSSLITGKEDAASNFARGYFGVGREMLDVALNRI 456
PR V++DLEP +D +++G + LF ++I G+ A + +A+G++ G E+ D L+ +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 457 RSVTEDCDNIQGFIVFRXXXXXXXXXXXXXXLESISRDFGKLSKIEYAIYPAPKISPIIV 636
R E CD +QGF + L I ++ +++ PAPK S +V
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 637 ESYNAVLTSHACMNTEDVCFLFDNEAL 717
E YNA L+ H + D F DNEAL
Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEAL 207
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 95.5 bits (227), Expect = 7e-21
Identities = 57/209 (27%), Positives = 104/209 (49%), Gaps = 5/209 (2%)
Frame = +1
Query: 106 KEILHIHIGQAGVQAANACWELYCLEHGIRPDGVL-AF-PDNDNSCGHFFSETGAGKFVP 279
+EI+ + GQ G Q + W+ CLEHGI PDG L +F + + FF ++ +++P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 280 RVVMIDLEPTPIDEIRTGVYRQLFHSSSLITGKE--DAASNFARGYFGVGREMLDVALNR 453
R ++IDLEP ++ I + Y L++ +++ K A +N+A GY R D+ ++
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDI-MDM 121
Query: 454 IRSVTEDCDNIQGFIVFRXXXXXXXXXXXXXXLESISRDFGKLSKIEYAIYP-APKISPI 630
I + D+++GF + LE ++ + K Y+++P + +S +
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 631 IVESYNAVLTSHACMNTEDVCFLFDNEAL 717
+V+ YN++L D + DN AL
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAAL 210
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.51
Identities = 35/121 (28%), Positives = 53/121 (43%), Gaps = 4/121 (3%)
Frame = -3
Query: 536 KPEPVPPPNARNTIKP*ILSQSSVTDRMRFKATSNISRPTPK*PRAKLLAASSLPVIREE 357
+P P PPP+ RN KP I + SS S++ P P PR+ AA S+P+ +
Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSS---------NSSLPPPPPP-PRSN--AAGSIPLPPQG 357
Query: 356 EWKSCRYTPVLISSMGVGSKSIITTRG-TNFPAP---VSLKKCPQLLSLSGNARTPSGLI 189
P S G + ++R +N PAP + + P L L +RT + +
Sbjct: 358 RSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPV 417
Query: 188 P 186
P
Sbjct: 418 P 418
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/45 (28%), Positives = 18/45 (40%), Gaps = 1/45 (2%)
Frame = +3
Query: 504 SSVWRGNRFWFHSFTSGKYFTRFWETVQNRV-RDLPSTENISNYC 635
SS+ FWF FT KY + + N V + P +C
Sbjct: 37 SSILNTTAFWFVEFTESKYDKEEFSVIWNEVSMEFPDIRRAKVFC 81
>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1496
Score = 27.1 bits (57), Expect = 2.7
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = -3
Query: 350 KSCRYTPVLISSMGVGSKSIITTRGTNFPAPVSLKKCPQLLSLSGNARTPSGLIPCS 180
KS T V + + +G + T + PV KK QL S ++ +PS +IP S
Sbjct: 398 KSLLNTVVGMDIVSLGKVPLFLTPIVRYKNPVKNKKRSQLTSRISSSVSPSNVIPNS 454
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 26.6 bits (56), Expect = 3.6
Identities = 20/71 (28%), Positives = 31/71 (43%)
Frame = -3
Query: 578 FPKSREILSRSKAVKPEPVPPPNARNTIKP*ILSQSSVTDRMRFKATSNISRPTPK*PRA 399
FP + L AVK +P PPP +N P + SS M+++ T+ + P P
Sbjct: 189 FPSNYVRLLEDSAVKAQPPPPPPQQN-YPP---AASSSAPPMQYQQTAYPPQQAPYPPVQ 244
Query: 398 KLLAASSLPVI 366
A P++
Sbjct: 245 AYPQAPQQPIV 255
>SPCC1902.02 |mug72|SPCC663.16c|ketopantoate reductase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 6.2
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 122 YTLDKPVCKPLTPVGNCIVLNT 187
Y LD + + +TP CIVLNT
Sbjct: 96 YNLDTAIKEVVTPGHTCIVLNT 117
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 25.8 bits (54), Expect = 6.2
Identities = 14/46 (30%), Positives = 20/46 (43%)
Frame = +1
Query: 187 GIRPDGVLAFPDNDNSCGHFFSETGAGKFVPRVVMIDLEPTPIDEI 324
G+ PDG F N N+ HF G F V+ D+ I+ +
Sbjct: 126 GLMPDGTSRFSCNGNTLLHFM---GCSTFSEYTVVADISVVAIERL 168
>SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 162
Score = 25.8 bits (54), Expect = 6.2
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 2/48 (4%)
Frame = -3
Query: 482 LSQSSVTDRMRFKATSNI--SRPTPK*PRAKLLAASSLPVIREEEWKS 345
L +S+ TD R+ N R + + +L ++ I+EEEWKS
Sbjct: 26 LQESTETDTSRYMDMENYIQKRVQDELKQLQLRQKKAIDAIQEEEWKS 73
>SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps901
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 537
Score = 25.8 bits (54), Expect = 6.2
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +1
Query: 253 ETGAGKFVPRVVMIDLEPTPIDEIRTGVYRQLFHSSSLITGK 378
E A FVP ++ + L+ P + Y Q F S +TG+
Sbjct: 292 EESADMFVPILIFVVLQARPAHLVSNIQYIQRFRSPEKLTGE 333
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,187,457
Number of Sequences: 5004
Number of extensions: 71297
Number of successful extensions: 220
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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