BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte26o02
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22H12.03 |||mitochondrial hydrolase|Schizosaccharomyces pomb... 70 3e-13
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 29 0.63
SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schiz... 27 2.6
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 26 4.5
SPAC1782.05 |||phosphotyrosyl phosphatase activator homolog|Schi... 26 4.5
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 7.8
SPBC106.11c |plg7||phospholipase A2 |Schizosaccharomyces pombe|c... 25 7.8
>SPAC22H12.03 |||mitochondrial hydrolase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 70.1 bits (164), Expect = 3e-13
Identities = 35/86 (40%), Positives = 46/86 (53%)
Frame = +1
Query: 235 PPLVILHGLLGSKNNWNSMSKAIHRTTGRKVISVDARNHGDSRHSPQHTYVHMAHDVMRX 414
PP++I HGLLGSK NW S++K R + ++D R HGDS +Y MA D +
Sbjct: 21 PPVLIFHGLLGSKRNWRSLAKKFSCKLDRDIYAIDQRCHGDSPCVAPLSYSAMALDAFQF 80
Query: 415 XXXXXXXXXXXXGHSMGGRTAMVLSL 492
GHSMG +TAMV +L
Sbjct: 81 MKDHKLDKASIIGHSMGAKTAMVTAL 106
Score = 37.1 bits (82), Expect = 0.002
Identities = 21/56 (37%), Positives = 34/56 (60%), Gaps = 2/56 (3%)
Frame = +3
Query: 525 DINLRNFLITNLVQ--TNAGSFTWRVNIPALKDNFQNHISSFPSNLKGLQYCGPTL 686
DI +R+FL++NL + N+ +F +RV I + + + I FP++L L Y PTL
Sbjct: 162 DILVRSFLLSNLKKDSNNSNTFKFRVPIELISKSLKT-IEGFPASLNDLVYDSPTL 216
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 29.1 bits (62), Expect = 0.63
Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -3
Query: 544 KFLRFISGVN-DLSWSYIAVIAPWQFGLPYCAQAVIPLTTLISLAAALHHE 395
K L++ S N +L ++ +IAP FG PY QA T I+L A E
Sbjct: 92 KVLQYCSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDE 142
>SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 547
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +3
Query: 615 DNFQNHISSFPSNLKGLQYCGPTL 686
D F N S FPS+L G+ P L
Sbjct: 200 DEFNNSSSGFPSSLNGIPIASPPL 223
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = +3
Query: 477 HGAITAMYDQLKSFTPDINLRNFLITNLVQTNAGSFTWRVNIPA 608
+G ++ +D+L S +NL + I L+Q N W V PA
Sbjct: 1409 NGVLSKAWDELSSAGSSVNLYSEEIILLIQKNLIVTKWDVKRPA 1452
>SPAC1782.05 |||phosphotyrosyl phosphatase activator
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 352
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +3
Query: 102 FQ*FHKITQCQCPKMLEFYSIERRNSRFGLRFLREHFGL*KFITTSSSH 248
FQ F+ + PK+ + + IE + R+ E FG K I S H
Sbjct: 93 FQSFYDQVYIETPKLHQVFGIEHNAAMEAGRYFYESFGNRKRIDYGSGH 141
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 25.4 bits (53), Expect = 7.8
Identities = 20/81 (24%), Positives = 37/81 (45%)
Frame = -3
Query: 622 KLSFRAGILTLQVKDPALVCTKLVIRKFLRFISGVNDLSWSYIAVIAPWQFGLPYCAQAV 443
K F ++ +++KD + T L++RKF +D Y ++ W + Y ++
Sbjct: 647 KEEFLYDLIQIRLKDDNVFPTLLLLRKF----DWKDDYQILYNTIMEVW--NIKY--NSL 698
Query: 442 IPLTTLISLAAALHHEPCVHM 380
L L+S H E C+H+
Sbjct: 699 NALARLLSALYKFHPEFCIHV 719
>SPBC106.11c |plg7||phospholipase A2 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 438
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/39 (30%), Positives = 25/39 (64%)
Frame = +1
Query: 235 PPLVILHGLLGSKNNWNSMSKAIHRTTGRKVISVDARNH 351
P + HGL+GS+N ++S+ I + G V++++ R++
Sbjct: 117 PVFIFSHGLVGSRNVYSSLCGTI-ASYGIVVLAMEHRDN 154
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,692,129
Number of Sequences: 5004
Number of extensions: 50588
Number of successful extensions: 125
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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