Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte25p15
         (441 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   177   6e-46
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    80   2e-16
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    75   6e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    50   1e-07
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy...    27   0.97 
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos...    27   1.3  
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce...    27   1.7  
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc...    26   2.2  
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos...    26   2.2  
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom...    26   2.2  
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb...    26   3.0  
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos...    26   3.0  
SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual    26   3.0  
SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom...    25   6.8  
SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces...    25   6.8  
SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos...    24   9.0  
SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|...    24   9.0  
SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa...    24   9.0  
SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharom...    24   9.0  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  177 bits (431), Expect = 6e-46
 Identities = 77/95 (81%), Positives = 89/95 (93%)
 Frame = +2

Query: 2   QNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNTTAIQELFKRISEQFTAMFRRKA 181
           Q KNS+YFVEWIP+NV  AVC +PP+ LKMSATFIGN+T+IQE+F+R+ +QF+AMFRRKA
Sbjct: 334 QTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKA 393

Query: 182 FLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDA 286
           FLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+A
Sbjct: 394 FLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEA 428


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 79.8 bits (188), Expect = 2e-16
 Identities = 35/97 (36%), Positives = 57/97 (58%), Gaps = 8/97 (8%)
 Frame = +2

Query: 8   KNSSYFVEWIPNNVKTAVCDIPPRGLKMS--------ATFIGNTTAIQELFKRISEQFTA 163
           K +  FV+W P   K  +CD PP+ ++ S           + NTT+I E + R+  +F  
Sbjct: 338 KRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDL 397

Query: 164 MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 274
           M+ ++AF+HWY GEGM+E EF+EA  ++  L  +Y++
Sbjct: 398 MYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 74.5 bits (175), Expect = 6e-15
 Identities = 33/99 (33%), Positives = 57/99 (57%), Gaps = 8/99 (8%)
 Frame = +2

Query: 2   QNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSAT--------FIGNTTAIQELFKRISEQF 157
           +++ +  FV+W P   K  +C  PP+ +  S           + NTT+I E + R+  +F
Sbjct: 340 KSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKF 399

Query: 158 TAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 274
             M+ ++AF+HWY GEGM+E EF+EA  ++  L  +Y++
Sbjct: 400 DLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 50.4 bits (115), Expect = 1e-07
 Identities = 28/93 (30%), Positives = 50/93 (53%), Gaps = 6/93 (6%)
 Frame = +2

Query: 23  FVEWIPNNVKTAVCDIPP---RGLKMSATFIGNTTAIQELFKRISEQFTAMFRRKAFLHW 193
           F+ W P +++ A+    P      ++S   + N T+I  LFKR  +Q+  + +R AFL  
Sbjct: 348 FIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQ 407

Query: 194 YTGEGMDEMEFTEAESNMN---DLVSEYQQYQD 283
           Y  E + E +  E +S+ +   DL++EY+  +D
Sbjct: 408 YKKEAIFEDDLNEFDSSRDVVADLINEYEACED 440


>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 648

 Score = 27.5 bits (58), Expect = 0.97
 Identities = 13/46 (28%), Positives = 23/46 (50%)
 Frame = -3

Query: 142 SFKQLLDSCGVSDEGGRHFESTRWNVAHGRLDVIGNPFHEVAAIFV 5
           +FK++   C V DE  R  + + +      L+++GNP H     F+
Sbjct: 265 NFKKI--KCLVLDEADRLLQKSHFEELSKLLEILGNPMHTQRQTFI 308


>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
           Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 27.1 bits (57), Expect = 1.3
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = +2

Query: 110 NTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 217
           N++ IQ L K I+   T  +R    ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228


>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1242

 Score = 26.6 bits (56), Expect = 1.7
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = -3

Query: 325  LYLFIKFPFIISRGVLILLVLRNQIVHVRFSLRELHFIHT 206
            LY+ I F  I +RG L L   +N ++ + F LR ++F  T
Sbjct: 1075 LYVAIPFRTIFNRGTLALDAFQNWVIGL-FMLRMIYFTVT 1113


>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
            Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1958

 Score = 26.2 bits (55), Expect = 2.2
 Identities = 18/59 (30%), Positives = 29/59 (49%)
 Frame = +2

Query: 104  IGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 280
            + N   +  L+K + E+F+ +F RK  L WY   G+      E + N+N   SE  + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756


>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 647

 Score = 26.2 bits (55), Expect = 2.2
 Identities = 18/73 (24%), Positives = 35/73 (47%)
 Frame = +3

Query: 99  PSSETPQLSKSCLKEFLNNLQPCSDEKLSCTGTRGRVWMKWSSRRLNRT*TIWFRSTSSI 278
           PSS+  ++ K  L  F+ N+    ++    T    +VW+  SS  L+   T W   +S++
Sbjct: 562 PSSDCSKILKCLLDGFVRNVAHLQNDGSYKTIGGKQVWLD-SSSVLHEKKTPWIMYSSAV 620

Query: 279 KTPRLMMKGNLMK 317
           ++   +   N+ K
Sbjct: 621 ESETQIFVKNISK 633


>SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1462

 Score = 26.2 bits (55), Expect = 2.2
 Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 3/49 (6%)
 Frame = -3

Query: 196  VPVQESFSSEHGCKL---FRNSFKQLLDSCGVSDEGGRHFESTRWNVAH 59
            +P  +S    H  +L   F    K  ++    S+E   HF+ TRW + +
Sbjct: 1216 LPFDKSILDNHSAELNDAFNLFLKAAVEYKVDSNESSEHFQDTRWKIIY 1264


>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 394

 Score = 25.8 bits (54), Expect = 3.0
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +3

Query: 81  DSKCLPPSSETPQLSKSCLKEFLNNLQPCSDE 176
           D +CLPPS ET    +  +KE  N ++  S E
Sbjct: 357 DMQCLPPSYETMGPCEKEMKEETNEVEIASIE 388


>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 25.8 bits (54), Expect = 3.0
 Identities = 12/36 (33%), Positives = 17/36 (47%)
 Frame = -1

Query: 441 FFFYLNTHFIKSLNKLTNITPNTKHQQVTNTEQDNI 334
           F+  LN H I    ++ NI PN    +VT    D +
Sbjct: 410 FYRLLNRHGIPVQERIVNIGPNKPENRVTKEMLDTL 445


>SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 25.8 bits (54), Expect = 3.0
 Identities = 15/56 (26%), Positives = 25/56 (44%)
 Frame = +3

Query: 9   KIAATSWNGFPITSRRPCATFHLVDSKCLPPSSETPQLSKSCLKEFLNNLQPCSDE 176
           K+ + SW    +T+ +   +  L     +   SETP +    +K+FLN      DE
Sbjct: 62  KLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPWIKMPVVKKFLNIKDESEDE 117


>SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1188

 Score = 24.6 bits (51), Expect = 6.8
 Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
 Frame = -3

Query: 355 KH*TGQHSLFLYLF-IKFPFIISRGVLILLVL 263
           K  TGQ+SLF Y+F +   +  +  ++I  VL
Sbjct: 158 KQKTGQYSLFAYIFSLVISWFFTHSIIISAVL 189


>SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces
          pombe|chr 2|||Manual
          Length = 224

 Score = 24.6 bits (51), Expect = 6.8
 Identities = 7/13 (53%), Positives = 10/13 (76%)
 Frame = -2

Query: 44 YWESIPRSSCYFC 6
          YW+SIP+  C +C
Sbjct: 4  YWKSIPKYYCKYC 16


>SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 891

 Score = 24.2 bits (50), Expect = 9.0
 Identities = 14/27 (51%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
 Frame = -3

Query: 88  FESTRWNVAHGRLDVIGNP--FHEVAA 14
           FES RW+V  G   V+GN   FH V A
Sbjct: 158 FESARWSVERG---VVGNSGLFHAVDA 181


>SPBP23A10.13 |orc4|orp4|origin recognition complex subunit
           Orc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 972

 Score = 24.2 bits (50), Expect = 9.0
 Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
 Frame = -3

Query: 145 NSFKQLLDSC-GVSDEGGRHFESTRWN 68
           + F+Q+L S   VSDEGG+    T WN
Sbjct: 750 SEFEQILKSVLYVSDEGGKDQIITCWN 776


>SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 639

 Score = 24.2 bits (50), Expect = 9.0
 Identities = 8/41 (19%), Positives = 22/41 (53%)
 Frame = +2

Query: 35  IPNNVKTAVCDIPPRGLKMSATFIGNTTAIQELFKRISEQF 157
           +P  V  ++ ++PP  ++   ++ G     +++F+   E+F
Sbjct: 591 VPRQVFASMLNLPPEVIRRKGSWTGKKDPREDMFRSRFEKF 631


>SPBC16C6.06 |pep1|vps10|sorting receptor for
           CPY|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1466

 Score = 24.2 bits (50), Expect = 9.0
 Identities = 9/25 (36%), Positives = 15/25 (60%)
 Frame = +2

Query: 215 EMEFTEAESNMNDLVSEYQQYQDAT 289
           E +F+ +ESN ND    Y +Y + +
Sbjct: 560 ECQFSNSESNKNDFEKWYTRYANGS 584


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,723,672
Number of Sequences: 5004
Number of extensions: 30823
Number of successful extensions: 105
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 160149590
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)