Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte25l20
(640 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 175 4e-45
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 175 4e-45
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 151 8e-38
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 150 2e-37
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 150 2e-37
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 90 3e-19
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 53 3e-08
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 1e-05
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 44 2e-05
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 42 6e-05
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 41 2e-04
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 38 0.002
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 36 0.004
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 34 0.015
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 33 0.026
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 29 0.43
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 28 1.3
SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre... 25 7.0
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 9.2
SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit... 25 9.2
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 9.2
SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac... 25 9.2
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 25 9.2
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 175 bits (426), Expect = 4e-45
Identities = 84/92 (91%), Positives = 88/92 (95%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 545 IQKESTLHLVLRLXGGTIEPSLRILAMKYNCE 640
IQKESTLHLVLRL GG IEPSL+ LA KYNCE
Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCE 92
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 175 bits (426), Expect = 4e-45
Identities = 84/92 (91%), Positives = 88/92 (95%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 545 IQKESTLHLVLRLXGGTIEPSLRILAMKYNCE 640
IQKESTLHLVLRL GG IEPSL+ LA KYNCE
Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCE 92
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 151 bits (366), Expect = 8e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = +2
Query: 323 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 502
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 63 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 122
Query: 503 GKQLEDGRTLSDYNIQKESTLHLVLRLXGG 592
GKQLEDGRTLSDYNIQKESTLHLVLRL GG
Sbjct: 123 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152
Score = 151 bits (366), Expect = 8e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = +2
Query: 323 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 502
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 139 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 198
Query: 503 GKQLEDGRTLSDYNIQKESTLHLVLRLXGG 592
GKQLEDGRTLSDYNIQKESTLHLVLRL GG
Sbjct: 199 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228
Score = 151 bits (366), Expect = 8e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = +2
Query: 323 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 502
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 215 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 274
Query: 503 GKQLEDGRTLSDYNIQKESTLHLVLRLXGG 592
GKQLEDGRTLSDYNIQKESTLHLVLRL GG
Sbjct: 275 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304
Score = 151 bits (366), Expect = 8e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = +2
Query: 323 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 502
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 291 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 350
Query: 503 GKQLEDGRTLSDYNIQKESTLHLVLRLXGG 592
GKQLEDGRTLSDYNIQKESTLHLVLRL GG
Sbjct: 351 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380
Score = 150 bits (363), Expect = 2e-37
Identities = 72/76 (94%), Positives = 75/76 (98%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 545 IQKESTLHLVLRLXGG 592
IQKESTLHLVLRL GG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 150 bits (363), Expect = 2e-37
Identities = 72/76 (94%), Positives = 75/76 (98%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 545 IQKESTLHLVLRLXGG 592
IQKESTLHLVLRL GG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 150 bits (363), Expect = 2e-37
Identities = 72/76 (94%), Positives = 75/76 (98%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 545 IQKESTLHLVLRLXGG 592
IQKESTLHLVLRL GG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
protein modifier Ned8|Schizosaccharomyces pombe|chr
2|||Manual
Length = 78
Score = 89.8 bits (213), Expect = 3e-19
Identities = 39/77 (50%), Positives = 56/77 (72%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
M I VKTLTGK I L+++ +D + +K ++++KEGIPP QQRLI+AGKQ+ D + Y+
Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60
Query: 545 IQKESTLHLVLRLXGGT 595
++ S LHLVL L GG+
Sbjct: 61 LEGGSVLHLVLALRGGS 77
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 53.2 bits (122), Expect = 3e-08
Identities = 21/42 (50%), Positives = 30/42 (71%)
Frame = +2
Query: 71 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIA 196
+ DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ +
Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFS 44
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 44.4 bits (100), Expect = 1e-05
Identities = 17/44 (38%), Positives = 29/44 (65%)
Frame = +2
Query: 71 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 202
++ D TR++ DK+ V+DF A WCGPCK + P L++++ +
Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63
>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 368
Score = 43.6 bits (98), Expect = 2e-05
Identities = 18/54 (33%), Positives = 35/54 (64%)
Frame = +2
Query: 395 KTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE 556
K + +V A I +K KIQ ++ ++Q+LI++G+ L D +T+ +YNI+++
Sbjct: 12 KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 42.3 bits (95), Expect = 6e-05
Identities = 21/73 (28%), Positives = 39/73 (53%)
Frame = +2
Query: 365 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 544
+ + +K + + V++ ++ +K I I ++QRLI+AG+ L+D +L Y
Sbjct: 4 ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63
Query: 545 IQKESTLHLVLRL 583
IQ ++HLV L
Sbjct: 64 IQDGHSIHLVKTL 76
>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 40.7 bits (91), Expect = 2e-04
Identities = 19/58 (32%), Positives = 33/58 (56%)
Frame = +2
Query: 392 GKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL 565
GK LE+E ++T +K ++ +PP++Q++I G QL+D L I+ +TL
Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 37.5 bits (83), Expect = 0.002
Identities = 15/45 (33%), Positives = 27/45 (60%)
Frame = +2
Query: 65 IHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA 199
+ ++ ++L+ + A K +I+F ATWCG CK + P +E+ A
Sbjct: 23 VELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGA 66
Score = 30.3 bits (65), Expect = 0.25
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 119 KLVVIDFMATWCGPCKMIGPKLDEI 193
K V+++F A WCG CK + P + +
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 36.3 bits (80), Expect = 0.004
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +2
Query: 116 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM 205
DK++++ F A WCG CK + P+ + A E+
Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADEL 68
Score = 34.3 bits (75), Expect = 0.015
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +2
Query: 119 KLVVIDFMATWCGPCKMIGPKLDEIAAE 202
K V+++F A WCG CK + P +++A E
Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEE 401
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 34.3 bits (75), Expect = 0.015
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 125 VVIDFMATWCGPCKMIGPKLDEIAAE 202
+ +D A WCGPCK I P ++A++
Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASK 47
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 33.5 bits (73), Expect = 0.026
Identities = 12/48 (25%), Positives = 25/48 (52%)
Frame = +2
Query: 59 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 202
MS+ I + + L +++++++F A W PCK + D+ A +
Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKD 48
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 29.5 bits (63), Expect = 0.43
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = +2
Query: 77 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEM 205
+S + + + G LVV F A WCG CK + P ++A+ +
Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNL 77
>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 27.9 bits (59), Expect = 1.3
Identities = 21/78 (26%), Positives = 36/78 (46%), Gaps = 2/78 (2%)
Frame = +2
Query: 368 QIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD--Y 541
+I V T+ K +V + T+ +K I P D+ +LI AG+ L + L + +
Sbjct: 5 RIRVTTVDQKVGIFQVPRTKTVLELKELIAVTFEAPADRLKLIHAGRVLRNETPLEEILH 64
Query: 542 NIQKESTLHLVLRLXGGT 595
+ T HLV+ + T
Sbjct: 65 DATDLVTFHLVIAVFNST 82
>SPBC19C2.09 |sre1||sterol regulatory element binding protein
Sre1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 900
Score = 25.4 bits (53), Expect = 7.0
Identities = 18/63 (28%), Positives = 26/63 (41%)
Frame = -2
Query: 462 LSWILALTFSIVSEASTSNVMVFPVRVLTNICIVLLK*TTRKIYDFLLPNWTVNECEDIG 283
+SW TF+ + + N +V + + I T KI D L +N C DIG
Sbjct: 833 ISWTALNTFAPICTSPKENDVVEKMAMYVRTAI-----GTLKIQDLKLSRKLINSCIDIG 887
Query: 282 IEL 274
L
Sbjct: 888 SRL 890
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 25.0 bits (52), Expect = 9.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 77 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA 199
+ D + RL EAG + +A CGPC + + D+IA+
Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIAS 466
>SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ysh1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 775
Score = 25.0 bits (52), Expect = 9.2
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 237 LMSARTSPASTTLTRCRCPRIHS 305
L+ +SPAS LT +C +HS
Sbjct: 610 LLGIESSPASVKLTSHKCNHLHS 632
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 25.0 bits (52), Expect = 9.2
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -1
Query: 445 FDIFDSVGSFHLQCNGLPRKGFDEYLH 365
F ++S+G + L C + FDEYL+
Sbjct: 2887 FKPYESLGKYMLSCLDDGKYAFDEYLY 2913
>SPAC19A8.12 |dcp2||mRNA decapping complex subunit
Dcp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 25.0 bits (52), Expect = 9.2
Identities = 14/52 (26%), Positives = 24/52 (46%)
Frame = +2
Query: 338 LRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRL 493
L V +S + + KTL +EA++ + +K Q K +PP + L
Sbjct: 618 LEVKNYSKSTDLLKKTLRIPRNDEPLEAANQFDLLKVSPQQKSEVPPKRNEL 669
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 25.0 bits (52), Expect = 9.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -1
Query: 439 IFDSVGSFHLQCNGLPRKGFDEYL 368
IF VG+F C+ L ++GF E L
Sbjct: 43 IFPGVGNFGFVCDSLAKQGFLEPL 66
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,726,756
Number of Sequences: 5004
Number of extensions: 54726
Number of successful extensions: 172
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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