Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte25k16
         (716 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   266   2e-72
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   138   6e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   137   2e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   133   2e-32
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple...    29   0.50 
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    28   1.2  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.5  
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb...    27   3.5  
SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s...    26   4.7  
SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch...    26   6.2  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        26   6.2  
SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe...    26   6.2  
SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch...    26   6.2  
SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosa...    25   8.2  
SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy...    25   8.2  
SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc...    25   8.2  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  266 bits (652), Expect = 2e-72
 Identities = 119/174 (68%), Positives = 137/174 (78%)
 Frame = +1

Query: 148 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYV 327
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D+ G Y G S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 328 PRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 507
           PRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 508 RKEAEGCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPK 669
           R+EAE CD LQGFQ                   KIREEYPDR+M TFSV P+PK
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174



 Score = 31.9 bits (69), Expect = 0.094
 Identities = 14/16 (87%), Positives = 15/16 (93%)
 Frame = +2

Query: 668 KVSDTVVEPYNATLSV 715
           K SDTVVEPYNATLS+
Sbjct: 174 KSSDTVVEPYNATLSM 189


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  138 bits (335), Expect = 6e-34
 Identities = 68/176 (38%), Positives = 96/176 (54%), Gaps = 2/176 (1%)
 Frame = +1

Query: 148 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLER--INVYYNEASGGK 321
           MREI+ I  GQ G QIG   WE+   EHGI   G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 322 YVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 501
           YVPR++ VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 502 VVRKEAEGCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPK 669
            +R+ A+ C  LQGF                    ++  EY  +    FSV P+P+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQ 176



 Score = 27.5 bits (58), Expect = 2.0
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +2

Query: 641 IRFPWYQVQKVSDTVVEPYNATLS 712
           ++F  Y   +VS +VVEPYN+ L+
Sbjct: 167 LQFSVYPAPQVSTSVVEPYNSVLT 190


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  137 bits (331), Expect = 2e-33
 Identities = 58/173 (33%), Positives = 105/173 (60%), Gaps = 2/173 (1%)
 Frame = +1

Query: 151 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYVP 330
           REI+ +QAGQCGNQIG++FW+ +  EHGI   G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 331 RAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 504
           RA+++DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D+
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 505 VRKEAEGCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPS 663
           + +EA+G D L+GF                    ++ + YP +I+ T+SV P+
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPN 174



 Score = 26.2 bits (55), Expect = 4.7
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +2

Query: 665 QKVSDTVVEPYNATLSV 715
           Q VSD VV+PYN+ L++
Sbjct: 176 QSVSDVVVQPYNSLLAL 192


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  133 bits (322), Expect = 2e-32
 Identities = 65/180 (36%), Positives = 98/180 (54%), Gaps = 6/180 (3%)
 Frame = +1

Query: 148 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLER------INVYYNEA 309
           MRE++ +  GQ G QIG   WE+   EHGI   G  + +S++             +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 310 SGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 489
             GK+VPR++ VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 490 SVLDVVRKEAEGCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPK 669
           SVL+ +R+ A+ C  LQGF                    ++  EY  +    FSV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180



 Score = 27.5 bits (58), Expect = 2.0
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +2

Query: 641 IRFPWYQVQKVSDTVVEPYNATLS 712
           ++F  Y   +VS +VVEPYN+ L+
Sbjct: 171 LQFSVYPAPQVSTSVVEPYNSVLT 194


>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
           subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1522

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 29/89 (32%), Positives = 35/89 (39%), Gaps = 4/89 (4%)
 Frame = -2

Query: 586 YQIQYLLPENELAENPADNRSLLLLF*LHPVPNQPVQPPLCNVLSPSYSPPQ---TVRRR 416
           Y   YL P   +A +P  N  L      H  P  P   PL N +SP Y P       R  
Sbjct: 26  YTGPYLTPSGTMAYHPG-NAPLFTQAPPHTNPQGPPPFPLFNSISPVYDPATGRLLYRNV 84

Query: 415 SCPVGRFA-QTARNGRSPWFQAQGPPSQL 332
           +  V   A     NG +  +   GPPSQL
Sbjct: 85  NTQVSHTAIPNPANGYAAVY--GGPPSQL 111


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 14/32 (43%), Positives = 17/32 (53%)
 Frame = -1

Query: 440 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 345
           P+   PK  L  R I PNGPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +1

Query: 382 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 483
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 782

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -3

Query: 699 LYGSTTVSETFWTWYHGKRIHNTIWILFTD 610
           + GST  ++ F   YH  R HNT++I  TD
Sbjct: 242 IVGSTLSADVF-ARYHRARNHNTLYICGTD 270


>SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3
           subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 821

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 10/35 (28%), Positives = 18/35 (51%)
 Frame = -3

Query: 216 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 112
           +F E+   + P   +LD+DD  +     + P E+N
Sbjct: 36  FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70


>SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 383

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 17/53 (32%), Positives = 26/53 (49%)
 Frame = +2

Query: 113 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 271
           F S  S     S   +STS RV++  ++  SSG  + T+  +   V  +AT T
Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +1

Query: 451 AKGHYTEGAELVDSVLDVVRKEAEGCDCLQ 540
           A+GH   G ELV +  D +RK++E    L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 522

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 9/21 (42%), Positives = 15/21 (71%)
 Frame = -2

Query: 580 IQYLLPENELAENPADNRSLL 518
           +QY+L +NEL  NP +  ++L
Sbjct: 342 VQYILEQNELGRNPYNTTNVL 362


>SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 383

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 17/53 (32%), Positives = 26/53 (49%)
 Frame = +2

Query: 113 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 271
           F S  S     S   +STS RV++  ++  SSG  + T+  +   V  +AT T
Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217


>SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 11/31 (35%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
 Frame = +2

Query: 560 LWEEVLDLVWGLF-*FPKSVKSIQIVL*IRF 649
           LW  V++++WGL   F  +V S++ ++ +RF
Sbjct: 98  LWFSVMNILWGLMTIFSFAVHSVRALMILRF 128


>SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 543

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 7/21 (33%), Positives = 14/21 (66%)
 Frame = -1

Query: 173 AWMWTISLMIALKIWNPPNRI 111
           AW+W ++LM+    +  PN++
Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478


>SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 329

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = -2

Query: 490 NQPVQPPLCNVLSPSYSPPQT 428
           N+P +PP+ N  +    PPQT
Sbjct: 86  NEPAKPPIPNAATKPTFPPQT 106


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,052,026
Number of Sequences: 5004
Number of extensions: 64260
Number of successful extensions: 223
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)