Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte23k03
(697 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 236 3e-63
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 149 3e-37
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 144 1e-35
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 84 2e-17
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 66 5e-12
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 60 2e-10
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 51 1e-07
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 32 0.068
SPBC1921.06c |pvg3|mug49|beta-1,3-galactosyltransferase |Schizos... 28 1.5
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 27 2.6
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 27 2.6
SPAC26H5.04 |||vacuolar import and degradation protein Vid28|Sch... 26 4.5
SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharo... 26 4.5
SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharo... 26 4.5
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 26 5.9
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 5.9
SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch... 26 5.9
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 5.9
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 7.8
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 236 bits (577), Expect = 3e-63
Identities = 104/179 (58%), Positives = 140/179 (78%)
Frame = +3
Query: 150 NSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEHVGKSKAQVA 329
++K+L+VGAGGIGCE+LKNL+++G + IIDLDTID+SNLNRQFLF K+HV + KA VA
Sbjct: 25 SAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHVKQPKAIVA 84
Query: 330 KDSALSFNPNVTIVAHHDSVISNDYGVKYFKQFNIVMNALDNRVARNHVNRLCLAADVPL 509
+A SFNPNV + A+H ++ + + V +F+QF++V NALDN AR HVN+ CL A VPL
Sbjct: 85 AKTASSFNPNVKLEAYHANIKEDRFNVAWFRQFDLVFNALDNLDARRHVNKQCLLASVPL 144
Query: 510 IETGTAGYAGQVELIKKGLTQCYECQPKAPQKSFPGCTIRNTPSEPIHCIVWGKTPLQP 686
IE+GT G+ GQV++I G T+CY+C PK P K++P CTIR+TPS+PIHC+VW K+ P
Sbjct: 145 IESGTTGFLGQVQVIIHGKTECYDCNPKEPPKTYPVCTIRSTPSQPIHCVVWAKSYFFP 203
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 149 bits (362), Expect = 3e-37
Identities = 70/187 (37%), Positives = 116/187 (62%), Gaps = 7/187 (3%)
Frame = +3
Query: 126 EKLTEAIANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEHV 305
E+ ++ +SK+L++GAGG+GCEILK+L L+GF + +ID+DTID++NLNRQFLF++ ++
Sbjct: 36 EETLKSAFSSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNI 95
Query: 306 GKSKAQVAKDSALSFNPNVTIVAHHDSVISNDYGVKYFKQFNIVMNALDNRVARNHVNRL 485
+ KA VA + P+ + + + D ++++K+F +++ LD+ AR +N
Sbjct: 96 DEPKANVAASMIMKRIPSTVVTPFYGKI--QDKTIEFYKEFKLIICGLDSVEARRWINST 153
Query: 486 CLAAD-----VPLIETGTAGYAGQVELIKKGLTQCYEC--QPKAPQKSFPGCTIRNTPSE 644
+A +PL++ G+ G GQ +I +T CYEC P+ S+P CT+ NTP
Sbjct: 154 LVAIAKTGDLIPLVDGGSEGLKGQARVIIPTITSCYECSLDMLTPKISYPICTLANTPRL 213
Query: 645 PIHCIVW 665
P HC+ W
Sbjct: 214 PEHCVEW 220
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 144 bits (348), Expect = 1e-35
Identities = 75/195 (38%), Positives = 115/195 (58%), Gaps = 10/195 (5%)
Frame = +3
Query: 117 VFDEKLTEAIANSKLLVVGAGGIGCEILKNLVLTGFP-----YIEIIDLDTIDVSNLNRQ 281
VF + E IA+ +VGAG IGCE+LKN + G +I + D+D+I+ SNLNRQ
Sbjct: 416 VFGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIEKSNLNRQ 475
Query: 282 FLFHKEHVGKSKAQVAKDSALSFNPNVT--IVAHHDSVISNDYGV---KYFKQFNIVMNA 446
FLF VGK K++ A + NP++T I ++ + V G+ ++F++ ++V NA
Sbjct: 476 FLFRPRDVGKLKSECASTAVSIMNPSLTGKITSYQERVGPESEGIFGDEFFEKLSLVTNA 535
Query: 447 LDNRVARNHVNRLCLAADVPLIETGTAGYAGQVELIKKGLTQCYECQPKAPQKSFPGCTI 626
LDN AR +V+R C+ + PL+E+GT G G +++ LT+ Y P+KSFP CT+
Sbjct: 536 LDNVEARMYVDRRCVFFEKPLLESGTLGTKGNTQVVVPHLTESYGSSQDPPEKSFPICTL 595
Query: 627 RNTPSEPIHCIVWGK 671
+N P+ H I W +
Sbjct: 596 KNFPNRIEHTIAWAR 610
Score = 48.8 bits (111), Expect = 7e-07
Identities = 31/150 (20%), Positives = 65/150 (43%)
Frame = +3
Query: 96 MVARVAGVFDEKLTEAIANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLN 275
+ +R V + + ++ S +L++G G+G EI KN+ L G + + D + +L+
Sbjct: 19 LYSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLS 78
Query: 276 RQFLFHKEHVGKSKAQVAKDSALSFNPNVTIVAHHDSVISNDYGVKYFKQFNIVMNALDN 455
Q+ ++ +G +A+V N V + SV+ ++ +Y K F V+ +
Sbjct: 79 SQYFLTEDDIGVPRAKVTVSKLAELNQYVPV-----SVV-DELSTEYLKNFKCVVVTETS 132
Query: 456 RVARNHVNRLCLAADVPLIETGTAGYAGQV 545
+ +N + I + G G +
Sbjct: 133 LTKQLEINDFTHKNHIAYIAADSRGLFGSI 162
>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 401
Score = 83.8 bits (198), Expect = 2e-17
Identities = 48/161 (29%), Positives = 78/161 (48%), Gaps = 1/161 (0%)
Frame = +3
Query: 141 AIANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEHVGKSKA 320
++ S +LV+GAGG+GC ++ LV G + I+D D +D SNL+RQ + G KA
Sbjct: 40 SLKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSNLHRQIIHSTSKQGMHKA 99
Query: 321 QVAKDSALSFNPNVTIVAHHD-SVISNDYGVKYFKQFNIVMNALDNRVARNHVNRLCLAA 497
AK NPNV I + + + SN + + +Q+++V++ DN+ R ++ C+
Sbjct: 100 ISAKQFLEDLNPNVIINTYLEFASASNLFSI--IEQYDVVLDCTDNQYTRYLISDTCVLL 157
Query: 498 DVPLIETGTAGYAGQVELIKKGLTQCYECQPKAPQKSFPGC 620
PL+ GQ+ + CY C P C
Sbjct: 158 GRPLVSASALKLEGQLCIYNYCNGPCYRCMFPNPTPVVASC 198
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 66.1 bits (154), Expect = 5e-12
Identities = 40/155 (25%), Positives = 71/155 (45%), Gaps = 1/155 (0%)
Frame = +3
Query: 141 AIANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEHVGKSKA 320
AI S + ++ A +GCE LKNL+L G ++D ++D S F + GKS+A
Sbjct: 24 AIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDFSMDGMNFFIQYDQEGKSRA 83
Query: 321 QVAKDSALSFNPNVTI-VAHHDSVISNDYGVKYFKQFNIVMNALDNRVARNHVNRLCLAA 497
+ NPNV + D ++YF +F++V+++ + +
Sbjct: 84 RCTASLLQQLNPNVEMEYLEMSPEALIDKNIEYFSKFSVVLSSNLKEKPLFRLEEYLRSH 143
Query: 498 DVPLIETGTAGYAGQVELIKKGLTQCYECQPKAPQ 602
+PL+ + G+AG + + T + QP+ PQ
Sbjct: 144 KIPLLHFNSVGFAGILRISTHEYTTT-QSQPELPQ 177
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 60.5 bits (140), Expect = 2e-10
Identities = 40/146 (27%), Positives = 69/146 (47%), Gaps = 1/146 (0%)
Frame = +3
Query: 93 KMVARVAGVFDEKLTEAIANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNL 272
+ +AR F E E + NS ++VVG GG+G ++ L +G I I+D D + +S+L
Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSL 166
Query: 273 NRQFLFHKEHVGKSKAQVAKDSALSFNPNVTIVAHHDSVISNDYGVKYFKQFNIVMNALD 452
NR + + VG K K + F P + + A + + + V++A+D
Sbjct: 167 NRHSIATLQDVGTPKTLAIKKAIKKFAPWIEVDARNALFNPDSADDLLSGNPDFVIDAID 226
Query: 453 NRVARNHVNRLCLAADVPLI-ETGTA 527
N + + C +P+I TG+A
Sbjct: 227 NIQTKVDLLSYCYNHKLPVIASTGSA 252
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 51.2 bits (117), Expect = 1e-07
Identities = 28/86 (32%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
Frame = +3
Query: 138 EAIANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEHVGKS- 314
+ I NSK L++GAG +GC + +NL+ G ++ +D T+ SN RQ LF E +
Sbjct: 331 DRIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVDYSTVSYSNPVRQSLFTFEDCKRKL 390
Query: 315 -KAQVAKDSALSFNPNVTIVAHHDSV 389
KA+ A PN+ ++ S+
Sbjct: 391 PKAECAAQRLKEIYPNMFSTGYNISI 416
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 32.3 bits (70), Expect = 0.068
Identities = 34/134 (25%), Positives = 56/134 (41%)
Frame = +3
Query: 147 ANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEHVGKSKAQV 326
A K+ +VG G G L L+ +P E I +D ++N++ +L K + K +
Sbjct: 1127 AREKVAIVGISGSGKSTLVELLRKTYPS-EDIYIDGYPLTNIDTNWLLKKVAIVDQKPHL 1185
Query: 327 AKDSALSFNPNVTIVAHHDSVISNDYGVKYFKQFNIVMNALDNRVARNHVNRLCLAADVP 506
+ L +S++ YGV + N VM+ALD + L D P
Sbjct: 1186 LGSTIL------------ESLL---YGVD--RDINSVMDALDKTYMTEVIQNLPNGLDTP 1228
Query: 507 LIETGTAGYAGQVE 548
L+E GQ++
Sbjct: 1229 LLEFSKNFSGGQIQ 1242
>SPBC1921.06c |pvg3|mug49|beta-1,3-galactosyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = +3
Query: 228 YIEIIDLDTIDVSNLNRQFLFHKEHVGKSKAQVAKD 335
Y I+ D NL R F KEHVGKS+ +D
Sbjct: 204 YDFIVKADDDSFLNLPRLFEMLKEHVGKSRFYFGRD 239
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 27.1 bits (57), Expect = 2.6
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 309 KSKAQVAKDSALSF-NPNVTIVAHHDSVISNDYGVKYFKQ 425
+++A V D L F NP T + +DSV S D+ ++Y Q
Sbjct: 608 RAQAMVDDDEFLEFANPEETKILKNDSVESYDWLIEYGSQ 647
>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 335
Score = 27.1 bits (57), Expect = 2.6
Identities = 15/53 (28%), Positives = 27/53 (50%)
Frame = +3
Query: 144 IANSKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSNLNRQFLFHKEH 302
+A K+ + G G IG +L+N +LTG + ++ ID+ + F + H
Sbjct: 1 MAIPKVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTH 53
>SPAC26H5.04 |||vacuolar import and degradation protein
Vid28|Schizosaccharomyces pombe|chr 1|||Manual
Length = 729
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = -3
Query: 548 LHLTSISCCTCLYKRYISSK--TQSINMVTGNTIVQCIHHNVELFEV 414
+ L SISC LYK I SK T+ I M+ +++ + E ++
Sbjct: 303 IRLLSISCLVTLYKAGILSKNQTKEIQMIVTPILIRLFFESREAKQI 349
>SPAC7D4.06c |alg3||dolichol-P-Man dependent
alpha|Schizosaccharomyces pombe|chr 1|||Manual
Length = 406
Score = 26.2 bits (55), Expect = 4.5
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +1
Query: 64 SLFDLSKYKSKWSRGSLAFSMRN*QKLLQILNCW*WAPAALVV 192
SLF LS K KW R S+ S+ K+ +L + PA LV+
Sbjct: 162 SLFILSSCKKKWVRASILLSVACSVKMSSLL----YVPAYLVL 200
>SPAC637.11 |suv3||ATP-dependent RNA helicase
Suv3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/62 (22%), Positives = 29/62 (46%)
Frame = -3
Query: 536 SISCCTCLYKRYISSKTQSINMVTGNTIVQCIHHNVELFEVFYTIVIADYTVMVSYDCYV 357
S T LY++ + +++N+ T N CI +LF + ++ D + +D Y
Sbjct: 448 SAGIVTTLYQKDFAKLNRAMNLPTKNLFNACIGAKDDLFFRYLSLFSDDIPQKLIFDRYF 507
Query: 356 RV 351
++
Sbjct: 508 KL 509
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +1
Query: 412 NTSNSSTL**MHWTIVLPVTMLIDCVLLLMYL 507
+TS S+ + WTIV V +L+ C+ L +L
Sbjct: 174 DTSESANSRVVRWTIVQIVVLLVTCIWQLSHL 205
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = -1
Query: 241 IISIYGKPVSTRFLRISQPMPPAPTTSNLEFAIASVNF 128
++ IY P++ F + P PP TSNL F I + F
Sbjct: 4 LVPIYTGPINPVFPSRAHPSPPLGLTSNL-FPIQTNKF 40
>SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +3
Query: 153 SKLLVVGAGGIGCEILKNLVLTGFPYIEIIDLDTIDVSN 269
S++ V+ +GG+ C +L L+ P E IDL + N
Sbjct: 254 SRVCVLYSGGVDCGVLARLMHDIVPNNESIDLINVAFEN 292
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 25.8 bits (54), Expect = 5.9
Identities = 19/95 (20%), Positives = 36/95 (37%)
Frame = -1
Query: 286 RN*RFKLLTSIVSRSIISIYGKPVSTRFLRISQPMPPAPTTSNLEFAIASVNFSSKTPAT 107
RN +S S +S YG R+++ P+ + S + S+N P
Sbjct: 151 RNSSRPFTSSFHSAPFLSSYGSSNDPSQERVNEYSLPSNSNSTYTTPLQSINNEHPLPTV 210
Query: 106 LATILIYTSTDRITKLCAKFVKVFNTNSTRIHK*P 2
L + ++ L + N + +R+H+ P
Sbjct: 211 LENNALLNNSGNSPSLINHLRQYLNQDFSRLHQSP 245
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 25.4 bits (53), Expect = 7.8
Identities = 26/102 (25%), Positives = 43/102 (42%), Gaps = 3/102 (2%)
Frame = +3
Query: 231 IEIIDLDTIDVSNLNRQFLFH-KEHVGKSKAQV--AKDSALSFNPNVTIVAHHDSVISND 401
+E+ D+ TI L R F +G +Q+ AKD S + T++ +N+
Sbjct: 414 LELFDVKTISFDPLTRHFAHRLSAELGNLMSQILAAKDRDPSTSYLKTVIFP-----TNE 468
Query: 402 YGVKYFKQFNIVMNALDNRVARNHVNRLCLAADVPLIETGTA 527
+ + I MN ARNH++ L D + GT+
Sbjct: 469 ---QTHRNARIAMNYYSRAAARNHIHSLIKIGDFYRMGLGTS 507
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,886,227
Number of Sequences: 5004
Number of extensions: 58302
Number of successful extensions: 203
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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