Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte23j09
(599 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 203 2e-53
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 195 5e-51
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 77 1e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 48 1e-06
SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr 3... 28 1.2
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 6.4
SPBP4G3.02 |pho1||acid phosphatase Pho1 |Schizosaccharomyces pom... 25 8.5
SPAC17G6.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 25 8.5
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 203 bits (495), Expect = 2e-53
Identities = 91/130 (70%), Positives = 107/130 (82%)
Frame = +1
Query: 1 RGDVVPKDVNAAIAAMKGRAGIRFVDWCPTGFKVGINYQPPSVVTGGDLAQVKRAASMLS 180
RGDV+P+DV AA+ ++K R I+FVDWCPTGFK+GI Y+PP V G +A+V RA MLS
Sbjct: 324 RGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLS 383
Query: 181 NTTAIAEAWGKLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYDEVAIET 360
NTT+IAEAW +LDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDY+EV ++
Sbjct: 384 NTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 443
Query: 361 SDMQPGADDE 390
D + DE
Sbjct: 444 MDNEMYEADE 453
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 195 bits (475), Expect = 5e-51
Identities = 85/123 (69%), Positives = 104/123 (84%)
Frame = +1
Query: 1 RGDVVPKDVNAAIAAMKGRAGIRFVDWCPTGFKVGINYQPPSVVTGGDLAQVKRAASMLS 180
RGDV+P+DV AA+ +K + I+FVDWCPTGFK+GI +PP + G ++A+V RA MLS
Sbjct: 320 RGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLS 379
Query: 181 NTTAIAEAWGKLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYDEVAIET 360
NTT+IAEAW +LDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDY+EV ++
Sbjct: 380 NTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439
Query: 361 SDM 369
++
Sbjct: 440 MEV 442
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 77.4 bits (182), Expect = 1e-15
Identities = 42/133 (31%), Positives = 70/133 (52%), Gaps = 3/133 (2%)
Frame = +1
Query: 1 RGDVVPKDVNAAIAAMKGRAGIRFVDWCPTGFKVGINYQPPSVVTGGDLAQVKRAASMLS 180
RG V K+V+ I +++ + FV+W P + PP +K +A+ +
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK--------DLKMSATFIG 369
Query: 181 NTTAIAEAWGKLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAR---EDLAALERDYDEVA 351
N+T+I E + +L +F M+ ++AF+HWY GEGM+E EF+EA DL + + Y E
Sbjct: 370 NSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAG 429
Query: 352 IETSDMQPGADDE 390
I+ D ++E
Sbjct: 430 IDEGDEDYEIEEE 442
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 48.0 bits (109), Expect = 1e-06
Identities = 29/117 (24%), Positives = 60/117 (51%), Gaps = 3/117 (2%)
Frame = +1
Query: 1 RGDVVPKDVNAAIAAMKGRAGIRFVDWCPTGFKVGINYQPPSVVTGGDLAQVKRAASMLS 180
+G+ P DV+ ++ ++ R F+ W P +V ++ + P + T ++ + ML+
Sbjct: 325 QGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----MLA 379
Query: 181 NTTAIAEAWGKLDHKFDLMYSKRAFVHWYVGEGMEE---GEFSEAREDLAALERDYD 342
N T+IA + + ++D + + AF+ Y E + E EF +R+ +A L +Y+
Sbjct: 380 NHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 27.9 bits (59), Expect = 1.2
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -2
Query: 331 VPELPNLLSLQRIRLPPSLRLHTNAQKLAWNT 236
+PELPNL +L+ + PPSL + + W T
Sbjct: 15 IPELPNLEALRSLWPPPSLN-ESGDTRSVWTT 45
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 6.4
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = -3
Query: 378 AGLHV*RLNSHFIVISFQSC-QIFSRFREFAFLHPFAYIPMHKSSLGIHEVELV 220
AG+ + LN + V S + C QIFS F E F HP + + + I ++++
Sbjct: 1708 AGILLTYLNQN--VSSLEKCNQIFSIFYEVFFQHPSTNVYANDEGIKIGALQII 1759
>SPBP4G3.02 |pho1||acid phosphatase Pho1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 453
Score = 25.0 bits (52), Expect = 8.5
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 5/35 (14%)
Frame = -3
Query: 93 TGRTPIHKPY-SGPALH----GRYSRVHVLRDHVS 4
T R+P HKPY GP++ + +VH L+ H S
Sbjct: 37 TSRSPYHKPYFYGPSIDFPTTCKIKQVHTLQRHGS 71
>SPAC17G6.11c |||glucosidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 636
Score = 25.0 bits (52), Expect = 8.5
Identities = 13/47 (27%), Positives = 20/47 (42%)
Frame = +1
Query: 136 GGDLAQVKRAASMLSNTTAIAEAWGKLDHKFDLMYSKRAFVHWYVGE 276
GG Q AS L+N+ A+ K + A + W+VG+
Sbjct: 475 GGVYQQALSCASQLNNSWLSGNAYQKYGFDYKPGSGPDALISWFVGD 521
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,411,596
Number of Sequences: 5004
Number of extensions: 49026
Number of successful extensions: 173
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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