Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte22n04
(683 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 288 4e-79
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 282 4e-77
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 149 3e-37
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 100 2e-22
SPBC2D10.13 |est1||telomerase regulator Est1|Schizosaccharomyces... 31 0.16
SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe... 27 2.5
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 27 3.3
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 26 4.4
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 25 7.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 288 bits (707), Expect = 4e-79
Identities = 125/213 (58%), Positives = 167/213 (78%)
Frame = +2
Query: 2 TYTNLNRLIAQVVSCMTASIRFKGSLNVDLVEFKTNLIPFPRIHFPLVTFAPFVSPSRAC 181
TY NLNRLIAQVVS +TAS+RF GSLNVDL EF+TNL+P+PRIHFPLVT++P VS ++A
Sbjct: 227 TYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAF 286
Query: 182 HETMTTNQLMMSCFEPANQMVKCDPRKGQYISCCLLFRGDVNPNDINATINHVKNSRSIK 361
HE+ + ++ CFEP NQMVKCDPR G+Y++ CLL+RGDV P D+ A + +K+ R+I+
Sbjct: 287 HESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQ 346
Query: 362 FVSWSPTGFKIGLNYQPPTTVPGGDLAAMQRAAVMVSNSSAIRVAWERICKKFSVMFAKR 541
FV W PTGFKIG+ Y+PP VPG +A + RA M+SN+++I AW R+ KF +M++KR
Sbjct: 347 FVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKR 406
Query: 542 AFVHHYIGEGLEEGEFSNALQNIKALSNDYKEI 640
AFVH Y+GEG+EEGEFS A +++ AL DY+E+
Sbjct: 407 AFVHWYVGEGMEEGEFSEAREDLAALERDYEEV 439
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 282 bits (691), Expect = 4e-77
Identities = 122/213 (57%), Positives = 166/213 (77%)
Frame = +2
Query: 2 TYTNLNRLIAQVVSCMTASIRFKGSLNVDLVEFKTNLIPFPRIHFPLVTFAPFVSPSRAC 181
+Y NLNRLIAQVVS +TAS+RF+GSLNVDL EF+TNL+P+PRIHFPLVT+AP VS ++A
Sbjct: 223 SYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAF 282
Query: 182 HETMTTNQLMMSCFEPANQMVKCDPRKGQYISCCLLFRGDVNPNDINATINHVKNSRSIK 361
HE+ + ++ CFEP NQMVKCDPR G+Y++ CLL+RGDV P D+ A + +K R+I+
Sbjct: 283 HESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQ 342
Query: 362 FVSWSPTGFKIGLNYQPPTTVPGGDLAAMQRAAVMVSNSSAIRVAWERICKKFSVMFAKR 541
FV W PTGFKIG+ +PP + G ++A + RA M+SN+++I AW R+ KF +M++KR
Sbjct: 343 FVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKR 402
Query: 542 AFVHHYIGEGLEEGEFSNALQNIKALSNDYKEI 640
AFVH Y+GEG+EEGEFS A +++ AL DY+E+
Sbjct: 403 AFVHWYVGEGMEEGEFSEAREDLAALERDYEEV 435
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 149 bits (362), Expect = 3e-37
Identities = 74/214 (34%), Positives = 124/214 (57%)
Frame = +2
Query: 2 TYTNLNRLIAQVVSCMTASIRFKGSLNVDLVEFKTNLIPFPRIHFPLVTFAPFVSPSRAC 181
+Y +LN L++ V++ +T S RF G LN DL + N++PFPR+HF +V FAP + +
Sbjct: 221 SYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSS 280
Query: 182 HETMTTNQLMMSCFEPANQMVKCDPRKGQYISCCLLFRGDVNPNDINATINHVKNSRSIK 361
+ ++ +L F+ N MV DPR G+Y++ LFRG V+ +++ I V+ S
Sbjct: 281 FQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAY 340
Query: 362 FVSWSPTGFKIGLNYQPPTTVPGGDLAAMQRAAVMVSNSSAIRVAWERICKKFSVMFAKR 541
FV W P + +VP DL + +A + NS++I+ + R+ +FS MF ++
Sbjct: 341 FVEWIPDNV-----LKAVCSVPPKDL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRK 392
Query: 542 AFVHHYIGEGLEEGEFSNALQNIKALSNDYKEIE 643
AF+H Y GEG++E EF+ A N+ L ++Y++ +
Sbjct: 393 AFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 100 bits (240), Expect = 2e-22
Identities = 60/222 (27%), Positives = 114/222 (51%), Gaps = 8/222 (3%)
Frame = +2
Query: 2 TYTNLNRLIAQVVSCMTASIRFKGSLNVDLVEFKTNLIPFPRIHFPLVTFAPF----VSP 169
T+ N+L++ V+S T ++R+ G +N DLV +LIP PR HF L ++ PF V
Sbjct: 224 TFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEE 283
Query: 170 SRACHETMTTNQLMMSCFEPANQMVKCDP-RKGQYISCCLLFRGDVNPNDINATINHVKN 346
++A +T T +M P NQMV +P +K +IS + +G+ +P D++ ++ ++
Sbjct: 284 AKAIRKT-TVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRE 342
Query: 347 SRSIKFVSWSPTGFKIGLNYQPPTTVPGGDLAAMQRAAVMVSNSSAIRVAWERICKKFSV 526
R F+ W P ++ L+ + P ++ + M++N ++I ++R ++
Sbjct: 343 RRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDR 397
Query: 527 MFAKRAFVHHYIGEGLEE---GEFSNALQNIKALSNDYKEIE 643
+ + AF+ Y E + E EF ++ + L N+Y+ E
Sbjct: 398 LRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACE 439
>SPBC2D10.13 |est1||telomerase regulator Est1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 490
Score = 31.1 bits (67), Expect = 0.16
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +1
Query: 34 GCFLHDCFDKIQRFSERRSRRI*NKLNSISTDTFSISNICAIC 162
G F HD ++KI +++ N+L SI T+TF +I C
Sbjct: 27 GGFKHDLYEKIIAIEIEVEKKLLNRLKSIQTNTFERPDIIWSC 69
>SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 959
Score = 27.1 bits (57), Expect = 2.5
Identities = 19/47 (40%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
Frame = +3
Query: 495 LGKEFAKNFL*CLPNALL-YTIILAKALKRVNSQMRCKILKLCPMTI 632
+G F+KNFL CL N L L +A KRV S++ K+ K P +
Sbjct: 312 IGDIFSKNFLHCLINHLSDEDRYLYRAAKRVTSKLE-KVSKQNPTLV 357
>SPCC663.03 |pmd1||leptomycin efflux transporter
Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1362
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -3
Query: 537 LANITENFLQILSQATRIAELFETITAALCIAARSPPGTVV 415
LANI+ +S A+ ++F+TI I A +P G VV
Sbjct: 373 LANISPKMQSFVSCASAAKKIFDTIDRVSPINAFTPTGDVV 413
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 26.2 bits (55), Expect = 4.4
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +2
Query: 287 LFRGDVNPNDINATINHVKNSRSIKFVSWSPTGFKIG 397
L GD+N N+ + I F+ WSPTG +IG
Sbjct: 346 LVSGDMNAAAGNSITFAEAIPQDIGFLPWSPTGGQIG 382
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 25.4 bits (53), Expect = 7.7
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -2
Query: 529 HHRKFFANSFPSNTYCGTV 473
HH +F NSF S+TY V
Sbjct: 744 HHLNYFMNSFLSSTYFDAV 762
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,681,219
Number of Sequences: 5004
Number of extensions: 56733
Number of successful extensions: 178
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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