Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte22h10
         (739 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    89   8e-19
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch...    69   7e-13
SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase Ubp12|Schizo...    36   0.005
SPCC16A11.12c |ubp1||ubiquitin C-terminal hydrolase Ubp1|Schizos...    33   0.042
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos...    33   0.056
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc...    32   0.074
SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa...    32   0.074
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch...    31   0.23 
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc...    30   0.40 
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos...    30   0.40 
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo...    29   0.69 
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc...    28   1.6  
SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit...    28   1.6  
SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch...    27   2.1  
SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces p...    27   3.7  
SPAC11H11.02c |mug162||sequence orphan|Schizosaccharomyces pombe...    27   3.7  
SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc...    27   3.7  
SPAC6G9.03c |mug183||histone chaperone Rtt106-like|Schizosacchar...    26   4.9  
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom...    26   4.9  
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C...    25   8.5  

>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 88.6 bits (210), Expect = 8e-19
 Identities = 50/121 (41%), Positives = 65/121 (53%), Gaps = 11/121 (9%)
 Frame = +3

Query: 408 WSCYGLGELKLLSYKPEGTHLCRKIHHMYHCKEDDWGFAHFIAWDDLMNPDNGF----VK 575
           +SC     L L + +          HH +  +  DWGF  F+    +  P   F    ++
Sbjct: 135 YSCCAQFALSLSNVQDPTVMQINTSHHRFRSEVKDWGFTRFVDLRKIAVPTPEFPVPFLE 194

Query: 576 NDSITLEAHV-IAEAPHGVSW------DSKKHTGYVGLKNQGATCYMNSLLQTLFFTNVL 734
           ND I +   V + + P GV W      +SKK TGYVGLKNQGATCYMNSLLQ+LFFTN+ 
Sbjct: 195 NDEICISVTVRVLQDPTGVLWHSFVNYNSKKETGYVGLKNQGATCYMNSLLQSLFFTNIF 254

Query: 735 R 737
           R
Sbjct: 255 R 255


>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
           Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1108

 Score = 68.9 bits (161), Expect = 7e-13
 Identities = 56/182 (30%), Positives = 85/182 (46%), Gaps = 11/182 (6%)
 Frame = +3

Query: 225 FRFIVQNVSELKEQVLSPACYVRCLPWKILVLVRNTTRNDHQPHKALGVFLQCNGECSSP 404
           F + +++  EL  + +SP   V    +KI    + T ++       L  ++    E  S 
Sbjct: 58  FTWHIKSWHELDRRAVSPQFAVGSRQFKITYFPQGTLQSAGFTSIFLE-YIPSEEEKLSN 116

Query: 405 GWSCYGLGELKLLSYKPEGTHLCRKIHHMYHCKEDDWGFAHFIAWDDLM---NPD-NGFV 572
            + C       + + +     +    H  +  +  DWGF  F     L+    PD    V
Sbjct: 117 KYGCCCQFAFVISNPRKPSLSVANSAHCRFSPEIVDWGFTQFAELKKLLCRQAPDVPPIV 176

Query: 573 KNDSITLEAHV-IAEAPHGVSW------DSKKHTGYVGLKNQGATCYMNSLLQTLFFTNV 731
           ++ ++ L A+V I + P GV W      DSK  TGYVGLKNQGATCYMNSLLQ+L+  + 
Sbjct: 177 EDGALLLTAYVRILKDPTGVLWHSFNDYDSKIATGYVGLKNQGATCYMNSLLQSLYIIHA 236

Query: 732 LR 737
            R
Sbjct: 237 FR 238


>SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase
           Ubp12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 979

 Score = 36.3 bits (80), Expect = 0.005
 Identities = 18/32 (56%), Positives = 19/32 (59%)
 Frame = +3

Query: 642 KKHTGYVGLKNQGATCYMNSLLQTLFFTNVLR 737
           K+  G  GL N G TCYMNS LQ L  T  LR
Sbjct: 305 KRVPGTCGLSNLGNTCYMNSALQCLTHTRELR 336


>SPCC16A11.12c |ubp1||ubiquitin C-terminal hydrolase
           Ubp1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 849

 Score = 33.1 bits (72), Expect = 0.042
 Identities = 15/30 (50%), Positives = 18/30 (60%)
 Frame = +3

Query: 645 KHTGYVGLKNQGATCYMNSLLQTLFFTNVL 734
           K  G  GL N G +CYMNS LQ +  T+ L
Sbjct: 274 KEIGLCGLYNLGNSCYMNSALQCMIHTHEL 303


>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
           Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 32.7 bits (71), Expect = 0.056
 Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
 Frame = +3

Query: 582 SITLEAHVIAEAPHGVSWDSKKHTGY--VGLKNQGATCYMNSLLQTLF 719
           S+ L+ H +  A       S  +  Y  +GL N G TCYMN +LQ LF
Sbjct: 44  SLKLDPHFMELANPKKVDSSLSYENYQPIGLTNLGNTCYMNCVLQCLF 91


>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
           Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1141

 Score = 32.3 bits (70), Expect = 0.074
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +3

Query: 660 VGLKNQGATCYMNSLLQTLFFTNVLR 737
           +GL+N G  CY+NSL+Q  F    LR
Sbjct: 614 IGLENTGNLCYLNSLIQYYFIIKPLR 639


>SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase
           Ubp7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 875

 Score = 32.3 bits (70), Expect = 0.074
 Identities = 17/36 (47%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
 Frame = +3

Query: 612 EAPHGVSWDSKKHTGYV-GLKNQGATCYMNSLLQTL 716
           ++ H     SKK+     GLKN GATC+ NS LQ L
Sbjct: 191 KSKHSSGKSSKKYKVISPGLKNLGATCFFNSTLQVL 226


>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
           Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 30.7 bits (66), Expect = 0.23
 Identities = 12/22 (54%), Positives = 16/22 (72%)
 Frame = +3

Query: 654 GYVGLKNQGATCYMNSLLQTLF 719
           G  GLKN G +CY+ S +Q+LF
Sbjct: 298 GLTGLKNLGNSCYLASTMQSLF 319


>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
           Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 615 APHGVSWDSKKHTGYV-GLKNQGATCYMNSLLQTL 716
           A   V  +  +  GY  GL N G TCYMNS +Q L
Sbjct: 84  AVESVEQEESEDDGYPSGLINLGNTCYMNSTVQML 118


>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
           Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 449

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 11/28 (39%), Positives = 20/28 (71%)
 Frame = +3

Query: 654 GYVGLKNQGATCYMNSLLQTLFFTNVLR 737
           G  G++N GATC+M+ +LQ++    ++R
Sbjct: 143 GLRGIQNLGATCFMSVILQSILHNPLVR 170


>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
           Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 457

 Score = 29.1 bits (62), Expect = 0.69
 Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
 Frame = +3

Query: 561 NGFVKNDSITLE-AHVI-AEAPHGVSWDSKKHTGYVGLKNQGATCYMNSLLQTL 716
           N FV  D   +  A ++  E    + WD+       GL N G TC+MNS+LQ +
Sbjct: 91  NDFVDEDPAFIPPARILFPEEKLSMEWDNIMPNA-PGLVNLGNTCFMNSVLQLM 143


>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
           Prp43|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
 Frame = +3

Query: 453 PEGTHLCR-KIHHMYHCKED--DWGFAHFIAWDDLMNPDN 563
           P+G HL    ++H Y   E   DW + HF++   L++ DN
Sbjct: 557 PDGDHLTLLNVYHAYKSGEGTADWCWNHFLSHRALISADN 596


>SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit
           Skp1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 161

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +3

Query: 120 AEMIEASSVDNATAPLPINHDFTPQDEDMARSE 218
           A MI   S ++      I +DFTP++E+  R E
Sbjct: 122 ANMIRGKSPEDIRKTFNIPNDFTPEEEEQIRKE 154


>SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 775

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +3

Query: 537 WDDLMNPDNGFVKNDSITLE 596
           W D+++PD  FVK  S+  E
Sbjct: 93  WSDILDPDADFVKQSSLAFE 112


>SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 612

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +2

Query: 215 GGHFPFYSSKCQRVERASAIP 277
           GG FP+  S+CQ +   S IP
Sbjct: 127 GGKFPYVFSECQAIHARSFIP 147


>SPAC11H11.02c |mug162||sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 264

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 18/41 (43%), Positives = 23/41 (56%)
 Frame = -1

Query: 211 RAISSSCGVKS*LMGRGAVALSTLLASIISAANLISYFNLL 89
           + I S   V   L G   + L+T   S+I+A  LISYFNLL
Sbjct: 192 KLIDSKDFVLGFLAGLSVLLLNT--GSLITAKPLISYFNLL 230


>SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase
           Ubp3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 512

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = +3

Query: 663 GLKNQGATCYMNSLLQTLFF 722
           G  N G  C+MNS+LQ L +
Sbjct: 134 GFINTGNICFMNSILQALMY 153


>SPAC6G9.03c |mug183||histone chaperone
           Rtt106-like|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 352

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 15/53 (28%), Positives = 25/53 (47%)
 Frame = -3

Query: 239 NYKTESGLRTGHIFVLRCEVMING*RSRCIIDAACFNHLSSESYILLQFTVHY 81
           NYK + G    H+F LR  ++    +    I+ A   H S  + +   FTV++
Sbjct: 206 NYKAKDG----HLFFLRTGILWGFRKPILFIELASIQHFSYSNVLQRTFTVNF 254


>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 860

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
 Frame = -2

Query: 510 RLLCNGTCDEFSCISAYPQAYRIGVLVRPIHNKTTQANCIR---RCTEGIRLKLCVVGDR 340
           ++L +   ++ SCI    +   IG L   I    ++   IR    C +GI  ++ +  D+
Sbjct: 34  KILHDSVGNKISCIGKSTKRIAIGTLDGRIVILNSRLQLIRDFYACEQGIVQQIYITADQ 93

Query: 339 FS*CCV 322
            + CCV
Sbjct: 94  SALCCV 99


>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
           Cct2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 13/43 (30%), Positives = 24/43 (55%)
 Frame = +3

Query: 117 AAEMIEASSVDNATAPLPINHDFTPQDEDMARSEATFRFIVQN 245
           A + + ASS+DN++ P     D     E++AR+  + + + QN
Sbjct: 133 AIDALRASSIDNSSDPAKFRSDL----ENIARTTLSSKILSQN 171


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,194,552
Number of Sequences: 5004
Number of extensions: 70744
Number of successful extensions: 201
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)