Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte22d15
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 37 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 33 0.039
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 31 0.12
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 30 0.28
SPCC4G3.09c |gyp3||GTPase activating protein Gyp3|Schizosaccharo... 27 2.0
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 2.6
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 27 3.4
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 3.4
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 6.0
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 25 7.9
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 25 7.9
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 25 7.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +2
Query: 506 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 664
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/50 (26%), Positives = 25/50 (50%)
Frame = +2
Query: 518 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 667
D DG+ + E ++ G +E +++ ++ D D +G+I EFL
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFL 71
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 33.1 bits (72), Expect = 0.039
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +2
Query: 500 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 667
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 30.7 bits (66), Expect = 0.21
Identities = 12/58 (20%), Positives = 25/58 (43%)
Frame = +2
Query: 494 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 667
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFV 96
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 31.5 bits (68), Expect = 0.12
Identities = 22/98 (22%), Positives = 45/98 (45%), Gaps = 1/98 (1%)
Frame = +2
Query: 389 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 568
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 569 KETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKIR 679
+ NK E + F +D D G IS E + ++
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLK 120
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 30.3 bits (65), Expect = 0.28
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +2
Query: 491 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSG 643
G+ ++FR + S +LN+ EF + GL + E LF + G
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG 538
>SPCC4G3.09c |gyp3||GTPase activating protein
Gyp3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 635
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 4/46 (8%)
Frame = +3
Query: 558 FMASRKQGWNLI----KVKPKNSSVNSTQTVVAQSVLMNSLLKSVP 683
FM+ K W L+ K PK N + QSVLM S+ +S+P
Sbjct: 418 FMSEEKAFWMLVITCRKYLPKMHDANLEGANIDQSVLMASVRESLP 463
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 326 KPKTSSNIAWHRPMSAGSVQEEELMQK 406
KPK S ++W+ + GS +E EL+ K
Sbjct: 56 KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/41 (29%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = +1
Query: 286 GCNVILNSKNVFRKTE--NIFQHCVAQTDVCWFRPGGRADA 402
GC + N+ E +F+ A + CW PG A+A
Sbjct: 331 GCRYVAEGSNMGSSAEAVEVFEKSRASGEGCWLAPGKAANA 371
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 3.4
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 7/46 (15%)
Frame = +2
Query: 581 LELNKSEA--EELFSQFDTDSSGSISLDEF-----LIKIRPSXVGI 697
L LN S EE F + D D SG +S +EF L+K R V I
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFVSLLKTRSEIVDI 371
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -3
Query: 563 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLGT 465
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 25.4 bits (53), Expect = 7.9
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +2
Query: 539 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFL 667
LNK EF K+ + S AE +F+ FD D +G I EF+
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFI 86
>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 312 KCI*ENRKHLPTLRGTDRCLLVPSRRK 392
+C+ E+R+H P L +D LL +RK
Sbjct: 82 RCMEEDREHNPFLSSSDNQLLSRKKRK 108
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 25.4 bits (53), Expect = 7.9
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +2
Query: 377 SVQEEELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEF 556
S Q E + R M L +L + ++ ILGL R D+G K+ NK+
Sbjct: 182 SYQNLESINSWLRLMASLYKDLSELVPVGITAEGRTILGLKLNGRHPSDNGEKIRNKKVI 241
Query: 557 L-----YGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 664
+ + + G+ A +L +++D+D LD+F
Sbjct: 242 IIQGGSHAREWIGIPSVCYAAWQLLAKYDSDGHVRKLLDKF 282
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,561,450
Number of Sequences: 5004
Number of extensions: 48905
Number of successful extensions: 149
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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