Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte22c09
         (743 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   268   7e-73
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   267   1e-72
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   144   1e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   122   5e-29
SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb...    27   2.1  
SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po...    26   4.9  
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ...    26   6.5  
SPBC16E9.03c |||DUF1783 family protein|Schizosaccharomyces pombe...    26   6.5  
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos...    25   8.6  
SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps90...    25   8.6  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  268 bits (656), Expect = 7e-73
 Identities = 124/209 (59%), Positives = 156/209 (74%), Gaps = 3/209 (1%)
 Frame = +3

Query: 126 MRECISVHVGQAGVQMGVACWQLYCLEHGIRPDG-TLPACETDPNTA--DSCFNTFFSEA 296
           MRE ISVHVGQAGVQ+G ACW+LYCLEHGI PDG      E   N +  +  F TFFSE 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 297 DRGKMVPRVVMVDLEATVIDEVRSGEYRQLYHPEQLITGKEDAANNYARGHYSTGREVLS 476
            +GK VPR + VDLE  VID+VR+G Y+ L+HPEQ++TGKEDA+NNYARGHY+ G+E++ 
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 477 PVMERIRKLADQCTGLQXXXXXXXXXXXXXXXXXXLLMEKLSDEFGKKSKLEFAIYPAPQ 656
            V+ERIR++AD C+GLQ                  LL+E+L+ E+GKKS L+F++YPAPQ
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180

Query: 657 VSTAVVEPYNAVLTTHATISHSDCAFMVD 743
           VST+VVEPYN+VLTTHAT+ +SDC FMVD
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVD 209


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  267 bits (654), Expect = 1e-72
 Identities = 122/206 (59%), Positives = 156/206 (75%)
 Frame = +3

Query: 126 MRECISVHVGQAGVQMGVACWQLYCLEHGIRPDGTLPACETDPNTADSCFNTFFSEADRG 305
           MRE IS+HVGQAG Q+G ACW+LYCLEHGI+P+G +   ET    +D  F+TFFSE  +G
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNP-ETASQNSDGGFSTFFSETGQG 59

Query: 306 KMVPRVVMVDLEATVIDEVRSGEYRQLYHPEQLITGKEDAANNYARGHYSTGREVLSPVM 485
           K VPR + VDLE  VID+VR+G YR L+HPEQLITGKEDA+NNYARGHY+ G+E++  V 
Sbjct: 60  KYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVT 119

Query: 486 ERIRKLADQCTGLQXXXXXXXXXXXXXXXXXXLLMEKLSDEFGKKSKLEFAIYPAPQVST 665
           ++IR++AD C+GLQ                  LL+E+L+ E+ KKSKL+F++YPAPQVST
Sbjct: 120 DKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179

Query: 666 AVVEPYNAVLTTHATISHSDCAFMVD 743
           +VVEPYN+VLTTHAT+  +DC FMVD
Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMVD 205


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  144 bits (349), Expect = 1e-35
 Identities = 72/206 (34%), Positives = 109/206 (52%)
 Frame = +3

Query: 126 MRECISVHVGQAGVQMGVACWQLYCLEHGIRPDGTLPACETDPNTADSCFNTFFSEADRG 305
           MRE + +  GQ G Q+G A W     EHG+   G         +      N +F+EA  G
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHER---LNVYFNEAAGG 57

Query: 306 KMVPRVVMVDLEATVIDEVRSGEYRQLYHPEQLITGKEDAANNYARGHYSTGREVLSPVM 485
           K VPR V+VDLE   +D V+SG++  L+ P+ +I G+  A N +A+GHY+ G E+   V+
Sbjct: 58  KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117

Query: 486 ERIRKLADQCTGLQXXXXXXXXXXXXXXXXXXLLMEKLSDEFGKKSKLEFAIYPAPQVST 665
           + +R+ A+ C  LQ                  LL+ K+ +E+  +    F++ PAP+ S 
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSD 177

Query: 666 AVVEPYNAVLTTHATISHSDCAFMVD 743
            VVEPYNA L+ H  + +SD  F +D
Sbjct: 178 TVVEPYNATLSMHQLVENSDETFCID 203


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  122 bits (294), Expect = 5e-29
 Identities = 68/208 (32%), Positives = 108/208 (51%), Gaps = 3/208 (1%)
 Frame = +3

Query: 129 RECISVHVGQAGVQMGVACWQLYCLEHGIRPDGTLPACETDPNTADSCFNTFFSEADRGK 308
           RE I++  GQ G Q+G   WQ  CLEHGI PDGTL +  T+        + FF ++D  +
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRK---DVFFYQSDDTR 59

Query: 309 MVPRVVMVDLEATVIDEVRSGEYRQLYHPEQLITGKE--DAANNYARGHYSTGREVLSPV 482
            +PR +++DLE  V++ + S  Y  LY+PE ++  K    A NN+A G YS    +   +
Sbjct: 60  YIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDI 118

Query: 483 MERIRKLADQCTGLQXXXXXXXXXXXXXXXXXXLLMEKLSDEFGKKSKLEFAIYPAPQ-V 659
           M+ I + AD    L+                   L+E+L+D + KK    ++++P  Q V
Sbjct: 119 MDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSV 178

Query: 660 STAVVEPYNAVLTTHATISHSDCAFMVD 743
           S  VV+PYN++L       ++D   ++D
Sbjct: 179 SDVVVQPYNSLLALKRLTLNADSVVVLD 206


>SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1283

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 17/71 (23%), Positives = 33/71 (46%)
 Frame = -2

Query: 388 YSWRYSPERTSSITVASKSTITTLGTIFPRSASEKNVLKQESAVLGSVSQAGRVPSGRIP 209
           Y+  Y P  T  +++   STI+TL T+     S  ++ K  S   G++++   + SG + 
Sbjct: 167 YTSTYLPNSTIDLSILPHSTISTLSTVSINDTS-ASLSKTTSPTAGTITET--IVSGSVG 223

Query: 208 CSKQYSCQHAT 176
            +  +     T
Sbjct: 224 YTSTFPASGTT 234


>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 942

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 20/66 (30%), Positives = 30/66 (45%)
 Frame = -2

Query: 400 CSG*YSWRYSPERTSSITVASKSTITTLGTIFPRSASEKNVLKQESAVLGSVSQAGRVPS 221
           C+   S R   +R S + VA  +  + L  I P     KN+L +   +   V+  G V  
Sbjct: 424 CNSLSSVRKLGKRKSIVIVAEGAIDSELNHISPEDI--KNLLVERLHLDTRVTTLGHVQR 481

Query: 220 GRIPCS 203
           G IPC+
Sbjct: 482 GGIPCA 487


>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1098

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 16/42 (38%), Positives = 19/42 (45%)
 Frame = +3

Query: 357  EVRSGEYRQLYHPEQLITGKEDAANNYARGHYSTGREVLSPV 482
            EVR  +    +  E LI GK+     Y R H     EVL PV
Sbjct: 1010 EVRGSQMIDTFSTELLIPGKQRVQRTYRRTH-----EVLEPV 1046


>SPBC16E9.03c |||DUF1783 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 249

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 11/33 (33%), Positives = 15/33 (45%)
 Frame = -3

Query: 249 QFHKRAGFRQVGSRAPSSIAASTRRPSGPRLGP 151
           + HKR GF  +      S+A+ T      R GP
Sbjct: 186 KLHKRQGFIDINFEVSGSLASGTVHYQSQRFGP 218


>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 731

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 10/15 (66%), Positives = 11/15 (73%)
 Frame = +1

Query: 481 LWSELGNLPINVPDY 525
           LWSELG  P +VP Y
Sbjct: 207 LWSELGVEPADVPQY 221


>SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps901
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 537

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 22/85 (25%), Positives = 41/85 (48%), Gaps = 4/85 (4%)
 Frame = +3

Query: 234 PACETDPNTADSCFNTFFSEADRGKMVPRVVMVDLEATVIDEVRSG--EYRQLY--HPEQ 401
           P+   DP   +S      +E  +G+      ++DL  + +DE R+   E+ + +   P Q
Sbjct: 4   PSFHEDPTKDESTV----AEQRKGQSNEEKPLIDLNDS-LDEQRNAYNEHCKNHDQQPSQ 58

Query: 402 LITGKEDAANNYARGHYSTGREVLS 476
            +   ED AN Y +   S+ +EV++
Sbjct: 59  QVRNMEDEANQYEQTDSSSDQEVMN 83


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,800,478
Number of Sequences: 5004
Number of extensions: 56808
Number of successful extensions: 174
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 353266144
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)