BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte22a24
(592 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.08 |||DUF1715 family protein|Schizosaccharomyces pombe|c... 38 0.001
SPAC2C4.12c |||tRNA 2'-phosphotransferase Tpt1 |Schizosaccharomy... 30 0.29
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 28 0.89
SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 27 1.5
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 3.6
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 3.6
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 26 3.6
SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 26 4.7
SPBC2F12.03c |||EST1 family protein|Schizosaccharomyces pombe|ch... 25 6.2
>SPCC191.08 |||DUF1715 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 134
Score = 37.5 bits (83), Expect = 0.001
Identities = 25/88 (28%), Positives = 39/88 (44%)
Frame = +1
Query: 253 DDIVLGEEKESKSSYDEGFRAGIEAGNIEGYHLGYHRGAELGRELGFYWSTVNECLELNE 432
+++ EE E K YDEG GIE G E + G G + V L+
Sbjct: 4 EEVTKLEENEYKRGYDEGILKGIEQGYEEAFLFGLEHAYNKYLLAGEIYGRVCFWLKEEN 63
Query: 433 TSDAKLSEKIIAQLLKVKELIDTFPPNN 516
+ K+ +K L ++K L+++ P NN
Sbjct: 64 SQHPKI-KKAHRHLEQLKSLLESLPTNN 90
>SPAC2C4.12c |||tRNA 2'-phosphotransferase Tpt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 361
Score = 29.9 bits (64), Expect = 0.29
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +1
Query: 250 LDDIVLGEEKESKSSYDEGFRAGIEAGNIEGYHLGYHRGAELGRELGFYWSTVNECLELN 429
+ ++ L EEK S Y EG G G+ G+ G+ LG ++G T+ L +
Sbjct: 262 MHELELLEEKHSNFGYSEGIIKGKMQVAQSGFDDGFKHGSRLGFQMGKTIGTLKAKLYIF 321
Query: 430 ETSD 441
E ++
Sbjct: 322 EENE 325
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 28.3 bits (60), Expect = 0.89
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -3
Query: 497 SISSLTLSSWAIIFSDNFA-SDVSFSSRHSFTVLQ 396
S SS+ LS+W+ IFS N + +D S H+ T+ +
Sbjct: 152 SQSSIFLSNWSTIFSSNASPTDSQLSPTHTSTIAE 186
>SPAC3C7.12 |tip1|noc1|CLIP170 family protein
Tip1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 461
Score = 27.5 bits (58), Expect = 1.5
Identities = 19/69 (27%), Positives = 36/69 (52%), Gaps = 10/69 (14%)
Frame = +1
Query: 373 LGRELGFYWSTVNECLELNET----SDAKLS------EKIIAQLLKVKELIDTFPPNNSE 522
L E FY S + + L+ N+T SD KLS E +++++ +++ I+ NN
Sbjct: 346 LSDEKDFYISKLEKSLDRNDTTPVPSDEKLSNYAAEKENLVSRISELEHTIEQLTINNER 405
Query: 523 DHDILGMAD 549
D++ + A+
Sbjct: 406 DNERMSPAE 414
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 26.2 bits (55), Expect = 3.6
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = -1
Query: 415 IHLLCSSRNLAHDPVQHPDDSPSDN 341
I LLC S N A DPV P + DN
Sbjct: 708 IRLLCYSSNNAIDPVSAPGNLDFDN 732
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 26.2 bits (55), Expect = 3.6
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 172 KIENGDHQNVAKLCLLMAVSKDFNDVLDDIVLG 270
KIE D +N KL L + +SKD+ +L + + G
Sbjct: 1677 KIEEPDLENNVKLILNLILSKDYGFLLKENLAG 1709
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 26.2 bits (55), Expect = 3.6
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = -2
Query: 411 IYCAPVET*LTTQFSTPMIAQVITLYVTSFYACSEPFIIARLAL 280
I+CA T T+F T MI+ I L+ S Y +P ++ L +
Sbjct: 76 IWCAGAGTAADTEFVTSMISSNIELH--SLYTNRKPRVVTALTM 117
>SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 941
Score = 25.8 bits (54), Expect = 4.7
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 81 IVRTVQFKNKKFVRCTLIV*V*NVYNL 161
+++ V F+ KKF C L+ V N Y +
Sbjct: 63 LLKEVSFQQKKFADCVLLTKVGNFYEM 89
>SPBC2F12.03c |||EST1 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 891
Score = 25.4 bits (53), Expect = 6.2
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -1
Query: 592 SLTKHKPSYIVL*LHRPYLVCHGLQNY 512
+LT HKP Y+VL ++R + + Y
Sbjct: 285 ALTDHKPQYLVLCVYRSLIYIGDVHRY 311
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,049,132
Number of Sequences: 5004
Number of extensions: 36062
Number of successful extensions: 103
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 256184654
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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