SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte21a12
         (651 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi...    32   0.062
SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||...    29   0.77 
SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc...    27   2.3  
SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||M...    27   2.3  
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe...    27   3.1  
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe...    27   3.1  
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB...    26   4.1  
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    26   5.4  
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom...    26   5.4  
SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom...    26   5.4  
SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch...    25   7.2  
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    25   9.5  
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb...    25   9.5  
SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   9.5  

>SPAC6B12.12 |tom70||mitochondrial TOM complex subunit
           Tom70|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 32.3 bits (70), Expect = 0.062
 Identities = 17/50 (34%), Positives = 27/50 (54%)
 Frame = +3

Query: 237 LGMMKQFVKALDKNNATFEYLCKKIPRLSDAKIKGVFDGPQIRSLMADEE 386
           L +   +VKAL++ +A +E L K    L D+ +  +FDG    S+ A  E
Sbjct: 211 LSLDSSYVKALNRRSAAYEQLGKLDEALMDSTVSCIFDGFANESMTATVE 260


>SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 923

 Score = 28.7 bits (61), Expect = 0.77
 Identities = 14/74 (18%), Positives = 34/74 (45%)
 Frame = +3

Query: 189 NLISPEKILLPPLHIKLGMMKQFVKALDKNNATFEYLCKKIPRLSDAKIKGVFDGPQIRS 368
           +LI+ + ++ P  +      +  +  L++ ++  +Y CKKI +    ++   +D  Q+  
Sbjct: 215 SLINTKTVIFPENYEDGPSFRSMLHELEQKSSLMKYYCKKIMK-RIVQLSDAYDASQVAV 273

Query: 369 LMADEEFDATMNNT 410
           +   E      N+T
Sbjct: 274 MKLSETLSEASNST 287


>SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 283

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 10/31 (32%), Positives = 18/31 (58%)
 Frame = +3

Query: 486 NKVANLLDKYQKLGCNMSIKLHFLDSHVDFF 578
           N+V N L   Q +  +   ++HFL++H+  F
Sbjct: 80  NRVCNALALLQCIASHPETRIHFLNAHITLF 110


>SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 556

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 11/29 (37%), Positives = 16/29 (55%)
 Frame = +3

Query: 240 GMMKQFVKALDKNNATFEYLCKKIPRLSD 326
           G +  F   LDK N TF+ +  + P LS+
Sbjct: 16  GNINNFSSFLDKENETFDVMMNEAPELSE 44


>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 560

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 6/61 (9%)
 Frame = -1

Query: 627 DETFLPALYCNH-----QDYQEK-SPHVNLESGVLCSYYTPIFDTYPISLQPYFYSRDVY 466
           +ETFLP L         Q+  EK S HV+ ES  +   +  ++D     L P    +  Y
Sbjct: 418 NETFLPKLKAERELLSKQESMEKQSMHVDEESNQILEKFLDVYDIIKAKLPPNVAEKVDY 477

Query: 465 Y 463
           Y
Sbjct: 478 Y 478


>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = +3

Query: 417 DAWLAFRDVVNNFLGNNKHPDYKNK 491
           D+W  F D  NN      HPDY+NK
Sbjct: 524 DSW--FNDTENNGHAQWVHPDYRNK 546


>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 588

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 14/47 (29%), Positives = 24/47 (51%)
 Frame = +3

Query: 450 NFLGNNKHPDYKNKVANLLDKYQKLGCNMSIKLHFLDSHVDFFPDNL 590
           +++ + KHP+YKN     ++KY K G     K+   D     F +N+
Sbjct: 78  HYVSSPKHPNYKN-----IEKYLKPGWLFGQKVDSADFQYSAFRENM 119


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
            Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 12/44 (27%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
 Frame = +1

Query: 481  IKIRLQTYWISI---KNWGVI*A*NSTF*IHMWTFFLIILVITV 603
            I + LQ YW+S    ++W  + + ++   +H W  FL++++  V
Sbjct: 2190 ISVFLQNYWMSSVYGRSWAFMWSAHN---VHKWAMFLLVILFYV 2230


>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 13/34 (38%), Positives = 17/34 (50%)
 Frame = +3

Query: 177 TLQKNLISPEKILLPPLHIKLGMMKQFVKALDKN 278
           T+ +N +   K   P LHI LG     VKA + N
Sbjct: 715 TMNENSVWYGKTCNPQLHITLGTSSSDVKAFESN 748


>SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 910

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 12/44 (27%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
 Frame = +3

Query: 402 NNTESDAWLAFRDVVNNFLGNN-KHPDYKNKVANLLDKYQKLGC 530
           +N+ +   L  +  VN  LG+   H D+KN++   ++ +++L C
Sbjct: 662 SNSVTHRALLLKLCVNKLLGSLLDHNDFKNRLETAIETFRELPC 705


>SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 752

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 15/44 (34%), Positives = 19/44 (43%)
 Frame = -3

Query: 379 SAIKDLICGPSNTPLIFASDNLGIFLHRYSKVALFLSNALTNCF 248
           +A  DL C  SN P + ASD L  F+           + L N F
Sbjct: 225 TAYWDLQCMCSNPPKLLASDTLPKFIDAAGSAIQAFESILQNTF 268


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1207

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 21/71 (29%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
 Frame = -3

Query: 439 SLNASQASDSV--LFIVASNSSSA-IKDLICGPSNTPLIFASDNLGIFLHRYSKVAL-FL 272
           +L  S A + V  L  V+S S+S  +KD I GP +  +  A +   +F      + +  L
Sbjct: 387 ALLTSSADNGVYELVNVSSRSNSLPLKDNIKGPGDDAIFVARNRFAVFSRSDQTIEIKDL 446

Query: 271 SNALTNCFIIP 239
           SN +T    +P
Sbjct: 447 SNKVTKTIQLP 457


>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1919

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = +3

Query: 381  EEFDATMNNTESDAWLAFRDVVNNFLGNNKHP 476
            EE  +++ ++E D  L  R V +  L N+KHP
Sbjct: 1774 EELFSSLKSSEEDFALRTRCVTSLMLVNSKHP 1805


>SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 773

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 10/34 (29%), Positives = 23/34 (67%)
 Frame = -2

Query: 614 SLLFTVITKIIRKKVHM*I*KVEFYAHITPQFLI 513
           SL+   I++I+++K  +   +V+FYA+  P+ ++
Sbjct: 695 SLVLPAISEIVKRKKDVHAERVQFYANPQPKAIV 728


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,738,816
Number of Sequences: 5004
Number of extensions: 59659
Number of successful extensions: 189
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -