Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte1e09
(749 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 355 4e-99
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 352 4e-98
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 187 2e-48
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 150 2e-37
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 31 0.23
SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces po... 30 0.40
SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p... 26 5.0
SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual 26 6.6
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 355 bits (873), Expect = 4e-99
Identities = 156/231 (67%), Positives = 189/231 (81%)
Frame = +1
Query: 52 MRECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGK 231
MRE ISIH GQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 232 HVPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 411
+VPR++++DLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 412 RVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQISTA 591
++R++AD C+GLQGFL+ LL+ERL+++Y KKSKL+F++YPAPQ+ST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 592 VVEPYNSILTTHTTLEHSDAAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 744
VVEPYNS+LTTH TL+ +D FMVDNE+ YDICRRNLDIERP+Y NLNRLI
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLI 231
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 352 bits (865), Expect = 4e-98
Identities = 156/235 (66%), Positives = 190/235 (80%), Gaps = 4/235 (1%)
Frame = +1
Query: 52 MRECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGD----DSFNTFFSET 219
MRE IS+H GQAGVQIGNACWELYCLEHGI PDG + V + D F TFFSET
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 220 GAGKHVPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVD 399
G GK VPR++++DLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 400 LVLDRVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 579
VL+R+R++AD C+GLQGFL+ LL+ERL+++YGKKS L+F++YPAPQ
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 580 ISTAVVEPYNSILTTHTTLEHSDAAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 744
+ST+VVEPYNS+LTTH TL++SD FMVDNEA YDICRRNLDIERPTY NLNRLI
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLI 235
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 187 bits (455), Expect = 2e-48
Identities = 92/231 (39%), Positives = 131/231 (56%)
Frame = +1
Query: 52 MRECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGK 231
MRE + I AGQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHG--TSEAQHERLNVYFNEAAGGK 58
Query: 232 HVPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 411
+VPRAV +DLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 412 RVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQISTA 591
VR+ A+ C LQGF + LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 592 VVEPYNSILTTHTTLEHSDAAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 744
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLV 229
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 150 bits (364), Expect = 2e-37
Identities = 82/233 (35%), Positives = 130/233 (55%), Gaps = 3/233 (1%)
Frame = +1
Query: 55 RECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGKH 234
RE I++ AGQ G QIG+ W+ CLEHGI PDG + S T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 235 VPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 408
+PRA+ IDLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 409 DRVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-IS 585
D + + AD L+GF + L+ERL+ Y KK ++++P Q +S
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 586 TAVVEPYNSILTTHTTLEHSDAAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 744
VV+PYNS+L ++D+ ++DN A+ I L + PT+ N+L+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLV 232
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 30.7 bits (66), Expect = 0.23
Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = +1
Query: 532 YGKKSKLEFAIYPAPQISTAVVE-PYNSILTTHTTLEHSDAAFMVDN 669
Y + K+E YP +I V + PYN +L ++LE ++DN
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKLLCPLSSLEWVSKLILMDN 156
>SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 29.9 bits (64), Expect = 0.40
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 530 STERRSIRSDAKPDPVPPPNEWKIRKP 450
S +RR ++++ PD V PP W +R P
Sbjct: 238 SDKRRFLKTNYSPDDVAPPPNWVLRYP 264
>SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 485
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = -2
Query: 112 NTRCRFAHQPGPRG*ICTHAFCFISILILQITRTFIF 2
N R + +Q R + A C + ILIL I + F F
Sbjct: 17 NDRKAYLNQWAKRSGLAIAAICILGILILAIVKLFCF 53
>SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 566
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = -1
Query: 143 WMPCSRQYSSQHALPICTPAWPAWIDMHS 57
W P H +PI TPA+P+ H+
Sbjct: 286 WNPKLYPSDKAHRMPIITPAYPSMCATHN 314
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,178,128
Number of Sequences: 5004
Number of extensions: 67004
Number of successful extensions: 213
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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