BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte16f20
(620 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3 |Sc... 30 0.31
SPBC947.08c |||histone promoter control protein Hpc2 |Schizosacc... 29 0.41
SPCC4G3.09c |gyp3||GTPase activating protein Gyp3|Schizosaccharo... 29 0.72
SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyc... 27 2.2
SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr 1|... 27 2.9
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 25 8.8
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 25 8.8
>SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 297
Score = 29.9 bits (64), Expect = 0.31
Identities = 17/66 (25%), Positives = 30/66 (45%), Gaps = 2/66 (3%)
Frame = +1
Query: 25 DGNMYAVFCLILISNYAFVQGEFVFKKKCRD--VDTSLCTVHNVMVEPCGEGPIFCALKK 198
D + Y C + + A GE + RD V ++ H ++ +P GP+ C L+K
Sbjct: 91 DCDQYCPKCSVELFLNAKCTGEGTMEIYARDLVVSSNSSLGHPILADPKSRGPLICKLRK 150
Query: 199 NKPYSI 216
+ S+
Sbjct: 151 EQEISL 156
>SPBC947.08c |||histone promoter control protein Hpc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 338
Score = 29.5 bits (63), Expect = 0.41
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +1
Query: 286 QNTFSTTFTRSAEYNDLLDNTLSEGKRTHIQLQLAVDKRASG 411
+ + + +++ NDL D+ SEG+ TH+ Q A + A G
Sbjct: 32 EKLYGPSVQENSKKNDLSDSADSEGESTHVDEQQAANGAADG 73
>SPCC4G3.09c |gyp3||GTPase activating protein
Gyp3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 635
Score = 28.7 bits (61), Expect = 0.72
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 383 WSCMCVRFPSDKVLSSRSLY 324
W C C++ PSD L R LY
Sbjct: 331 WRCACIKKPSDSDLIERDLY 350
>SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 364
Score = 27.1 bits (57), Expect = 2.2
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = +1
Query: 184 CALKKNKPYSISLDVTPHFSANKLHAVIKGDVQNQNTFSTTFTRSAEYNDLLDNTLSEGK 363
CA KK + SL +P+ + +L + + ++ STT R + L + GK
Sbjct: 237 CASKKETLHIFSLHGSPN-TIRQLTSEEPYEEASEFKSSTTEPRQTHWKRKLLKLIDSGK 295
Query: 364 RTHIQLQL 387
R H ++QL
Sbjct: 296 RAHWRIQL 303
>SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +2
Query: 305 PSRDQQSTMTYWTILYRKGSEHTYNSSWLW 394
P+RD M W I Y S H Y+ W W
Sbjct: 502 PARDLGPRMFAWWIGYGPHSFHLYHWWWTW 531
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 25.0 bits (52), Expect = 8.8
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = -3
Query: 534 LLHDNIFSQELHNLSDFNRKY*ATNMRRIILV 439
+L + S LHN S+ KY A +R++ L+
Sbjct: 873 ILESRLLSNPLHNFSELEEKY-AEYLRKLALL 903
>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 504
Score = 25.0 bits (52), Expect = 8.8
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 5 FSSTTAPTETCTPYFVLF*FRITLLFRASLYSRKNVEMWTLHCV 136
FS ++T TP + + + LYS KNV+MW + CV
Sbjct: 241 FSKVVWNSKTATPCGTVG-YAAPEIVNDELYS-KNVDMWAMGCV 282
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,420,785
Number of Sequences: 5004
Number of extensions: 51032
Number of successful extensions: 143
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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