BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte15m07
(677 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 121 1e-28
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 81 2e-16
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 50 3e-07
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 38 0.001
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 28 1.4
SPBP23A10.02 |||conserved fungal protein|Schizosaccharomyces pom... 27 3.3
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 27 3.3
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 5.8
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 7.6
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 25 7.6
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 121 bits (291), Expect = 1e-28
Identities = 58/178 (32%), Positives = 98/178 (55%)
Frame = +3
Query: 144 VSNAYSYTVTVDAHAEECFYENVEADTKLGLSFEIAEGGFLDIDVIILGPDGNVIHKAER 323
+S + + +T+ H ECFYEN+ + ++ ++++ GG + + I P G ++H+
Sbjct: 16 ISVVFGHGITLKPHQRECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVP 75
Query: 324 ESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNENEADHNKLEDMI 503
S Y+F+ GKY YCF N K V+FN+ +E+ +N L +
Sbjct: 76 NSMAQYSFTVKNPGKYMYCFYNDALDGESKEVLFNV---HGVIYISDEDLDANNPLLGKV 132
Query: 504 KELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYLK 677
++L T+ +KH+QEY R+RIHR+ ESTN RV WSI + +L+ + + Q++YLK
Sbjct: 133 RQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLK 190
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 80.6 bits (190), Expect = 2e-16
Identities = 42/191 (21%), Positives = 92/191 (48%), Gaps = 5/191 (2%)
Frame = +3
Query: 120 LVVTLLNLVSNAYSYTVTVDAHAEECFY-ENVEADTKLGLSFEIAEGGFLDIDVIILGPD 296
+ + LL +++A T ++ ++C+Y ++ K ++ + GG D+D +I P
Sbjct: 9 ITLFLLASLASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPS 68
Query: 297 GNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNL----EIGEPQTKSGN 464
+ ++ F+ G+Y +CF N MS+ T K+V + E+ P
Sbjct: 69 QKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDE 128
Query: 465 ENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLL 644
+ + ++ + E++T L I Q Y + R+ + S +ST +R+ +S+ E+ +++
Sbjct: 129 AKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVV 188
Query: 645 VMTIGQVYYLK 677
++ QV+ +K
Sbjct: 189 ALSALQVFIVK 199
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 50.0 bits (114), Expect = 3e-07
Identities = 34/131 (25%), Positives = 58/131 (44%), Gaps = 3/131 (2%)
Frame = +3
Query: 294 DGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGN--- 464
+ ++I+K + S+ F+ G + C+ + L + N
Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135
Query: 465 ENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLL 644
+E + L D + L T L +I +Q + R+ R +ES NSRVV W+I + VLL
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLL 195
Query: 645 VMTIGQVYYLK 677
V I Q+ +L+
Sbjct: 196 VTCIWQLSHLQ 206
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 38.3 bits (85), Expect = 0.001
Identities = 25/69 (36%), Positives = 35/69 (50%)
Frame = +3
Query: 471 EADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVM 650
EAD + D I+E IK Y+Q R+ R+ NESTN RV ++ L V+
Sbjct: 145 EADIRRARDFIEE-------IKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVL 197
Query: 651 TIGQVYYLK 677
I Q+ YL+
Sbjct: 198 VIWQILYLR 206
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.9 bits (59), Expect = 1.4
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
Frame = -2
Query: 658 PIVMTSSTEASNID---HITTREFVLSFIERWIRSRTCIYS*SCLIVL 524
P +T + EA ID + E V+ F+E SR C+Y SC+ +L
Sbjct: 195 PFFLTHNAEADAIDLLQELGAIEKVVPFVELDNASRVCLYITSCVNLL 242
>SPBP23A10.02 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 132
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/42 (26%), Positives = 23/42 (54%)
Frame = +3
Query: 456 SGNENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRS 581
S N+N +++ ++ D + ATT +T +E ++ R + S
Sbjct: 91 SNNQNPSNNKEMSDKESDSATTTQTFSVPEELLRARTTANNS 132
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +1
Query: 292 LMATSFIKQRGSHLVFIHFLLQLQGSILIASVIKCHP 402
+M T +K G + +HF+ + G+ L ++ KC P
Sbjct: 170 VMVTEALKPYGQENLELHFVSNIDGTHLAEALKKCDP 206
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2
Query: 157 YALLTRFNKVTTSHCDILKYFILINV 80
YA+L N +T++ CD Y +L+ V
Sbjct: 1026 YAILNILNLLTSNECDEFDYSVLMKV 1051
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +3
Query: 477 DHNKLEDMIKELATTLRTIK 536
DH+KL+D +++ A+TL IK
Sbjct: 723 DHSKLDDDLEKFASTLDPIK 742
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 25.4 bits (53), Expect = 7.6
Identities = 8/37 (21%), Positives = 21/37 (56%)
Frame = +3
Query: 468 NEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHR 578
N+A + K +D I++ +T++ +Y+ ++H+
Sbjct: 9 NDAKYRKYQDAIRKCIALFQTVEQWPDYISFLTKLHK 45
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,705,674
Number of Sequences: 5004
Number of extensions: 53875
Number of successful extensions: 140
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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