BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte12g04
(628 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 0.96
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 1.7
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 27 2.2
SPAC824.06 |tim14||TIM23 translocase complex subunit Tim14|Schiz... 26 3.9
SPAC22E12.10c |etp1|cox15|mitochondrial type I [2Fe-2S] ferredox... 26 3.9
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 26 3.9
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 6.8
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.8
SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 6.8
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 6.8
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 28.3 bits (60), Expect = 0.96
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +3
Query: 276 AIDLLTNDECRLLLEVEDFFN 338
+I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 1.7
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 409 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 498
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 27.1 bits (57), Expect = 2.2
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 547 SSKTFLKFLNAWTHNPPAAVLASSPF 624
SS +F F NA NPP++ L ++PF
Sbjct: 345 SSNSFSLFPNATLPNPPSSELLTTPF 370
>SPAC824.06 |tim14||TIM23 translocase complex subunit
Tim14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 140
Score = 26.2 bits (55), Expect = 3.9
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -3
Query: 620 GLEARTAAGGLWVHAFRNFRNV 555
G+ A AAG + V AFR +RN+
Sbjct: 10 GIAATAAAGKIGVDAFRKYRNL 31
>SPAC22E12.10c |etp1|cox15|mitochondrial type I [2Fe-2S] ferredoxin
Etp1/ cytochrome oxidase cofactor Cox15,
fusion|Schizosaccharomyces pombe|chr 1|||Manual
Length = 631
Score = 26.2 bits (55), Expect = 3.9
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -2
Query: 117 ILCVKAHAGVQTIRY*KPIVIAKLHEKTKIRLLTAS 10
++ +A G+ T+ Y P+ +A LH+ + LTA+
Sbjct: 422 VVTAQATLGIMTLIYVVPVPLAALHQAGSLVTLTAA 457
>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 3.9
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +2
Query: 38 FSCNFAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 211
F C I++ F I A A H I L + P +D H K I+P LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366
Query: 212 FAFVELNHYNNECESEGVMGCGHRF 286
F F E NH +++ ++ G ++
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAAGWKW 391
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 25.4 bits (53), Expect = 6.8
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +3
Query: 477 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 593
+D CKQ+ +FS E++ D L SI +S + ++ P
Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 6.8
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 294 NDECRLLLEVEDFFNDDC 347
NDECR L + FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552
>SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 95
Score = 25.4 bits (53), Expect = 6.8
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +2
Query: 149 DKLVHLHKTPTSLDINPSGLLFAFVELNH 235
D + H K +S NPS LLF L H
Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87
>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 6.8
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +2
Query: 125 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 259
L + ++KLV+L T T LD + ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,473,472
Number of Sequences: 5004
Number of extensions: 47419
Number of successful extensions: 134
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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