Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte12e24
(565 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 140 2e-34
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 50 2e-07
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 49 4e-07
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 47 2e-06
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|... 47 2e-06
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 44 1e-05
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 42 6e-05
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 39 6e-04
SPAC644.04 |pct1||RNA 5'-triphosphatase|Schizosaccharomyces pomb... 29 0.62
SPBC115.03 ||SPBC839.18c|gfo/idh/mocA family oxidoreductase |Sch... 27 2.5
SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 25 5.8
SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 25 5.8
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 25 7.7
SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|S... 25 7.7
>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 579
Score = 140 bits (338), Expect = 2e-34
Identities = 61/156 (39%), Positives = 91/156 (58%), Gaps = 1/156 (0%)
Frame = +2
Query: 92 IKQDWWETAVFYQLNTRSFMXXXXXXXXXXXXXTTRLDYLKELGVDAAWLSPIFKSAMHD 271
IK +WW YQ+ SF +++DYLK L V++ WL PI+ S + D
Sbjct: 9 IKPNWWRETSVYQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPSPLKD 68
Query: 272 FGYDTTDYYTIQPEYGSMEDFEHLLKKANELNIKIVLELVPNHTSNESEWFLKS-SNRDE 448
GYD +DY I YG++ED + L+K +E ++K+V++LV NHTS++ EWF +S S++
Sbjct: 69 MGYDVSDYKQIDSRYGTLEDLDRLMKALHERDMKLVMDLVLNHTSDQHEWFKESRSSKTN 128
Query: 449 YYSDWFIWESGHLDNMGIRKPPNNWVSVFRKSAWKY 556
DW+ W+ + G R PPNNW S F SAW++
Sbjct: 129 PKRDWYFWKPARYNEKGERLPPNNWRSYFDTSAWEW 164
>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 478
Score = 50.4 bits (115), Expect = 2e-07
Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 12/103 (11%)
Frame = +2
Query: 191 TTRLDYLKELGVDAAWLSPIFKSAMHD-------FGYDTTDYYTIQPEYGSMEDFEHLLK 349
T LDY+K LG A W+SPI K+ GY D + +G+ ED + L+
Sbjct: 57 TRNLDYIKSLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEEDLKELVN 116
Query: 350 KANELNIKIVLELVPNHTSNESEW---FL--KSSNRDEYYSDW 463
+E N+ ++++V NH + FL + N +YY +W
Sbjct: 117 AIHEKNMLCMVDIVVNHMGHAGSKPVNFLLYQPFNSGKYYHNW 159
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 49.2 bits (112), Expect = 4e-07
Identities = 24/73 (32%), Positives = 45/73 (61%), Gaps = 7/73 (9%)
Frame = +2
Query: 200 LDYLKELGVDAAWLSPIFKSAMHD-------FGYDTTDYYTIQPEYGSMEDFEHLLKKAN 358
LDY++ +G DA W+SPIF++ + GY TT+ Y + +G+ E+F L+++ +
Sbjct: 75 LDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEEFMELIQELH 134
Query: 359 ELNIKIVLELVPN 397
+ +I I+L++ N
Sbjct: 135 KRDIWILLDVAIN 147
>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 564
Score = 47.2 bits (107), Expect = 2e-06
Identities = 23/75 (30%), Positives = 43/75 (57%), Gaps = 8/75 (10%)
Frame = +2
Query: 197 RLDYLKELGVDAAWLSPIFKSAMHDF--------GYDTTDYYTIQPEYGSMEDFEHLLKK 352
+LDY++++G +A W+SPI K+ D GY TDY ++ +G+ +D L+
Sbjct: 69 KLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLITA 128
Query: 353 ANELNIKIVLELVPN 397
A++ I ++L+ + N
Sbjct: 129 AHKAGIWVMLDSIVN 143
>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 491
Score = 46.8 bits (106), Expect = 2e-06
Identities = 24/83 (28%), Positives = 45/83 (54%), Gaps = 9/83 (10%)
Frame = +2
Query: 197 RLDYLKELGVDAAWLSPIFKS---------AMHDFGYDTTDYYTIQPEYGSMEDFEHLLK 349
+LDY++ LG A W+SPI K+ A H GY D + P +G+ +D L+
Sbjct: 64 KLDYIQSLGCTAVWISPIVKNIEGVTGYGEAYH--GYWAEDLTQLNPHFGTKQDLTELVD 121
Query: 350 KANELNIKIVLELVPNHTSNESE 418
+ ++ N+ ++++V NH ++ +
Sbjct: 122 QLHKRNMLCMIDIVVNHMAHAGD 144
>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
Mde5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 44.4 bits (100), Expect = 1e-05
Identities = 24/77 (31%), Positives = 41/77 (53%), Gaps = 9/77 (11%)
Frame = +2
Query: 197 RLDYLKELGVDAAWLSPIFKS---------AMHDFGYDTTDYYTIQPEYGSMEDFEHLLK 349
+LDY++ +G A W+SPI K+ A H GY D YT+ P +G+ +D L
Sbjct: 70 KLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYH--GYWPQDLYTLNPHFGTEQDLIDLAD 127
Query: 350 KANELNIKIVLELVPNH 400
++ + ++++ V NH
Sbjct: 128 ALHDRGMYLMVDTVVNH 144
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 41.9 bits (94), Expect = 6e-05
Identities = 27/105 (25%), Positives = 55/105 (52%), Gaps = 7/105 (6%)
Frame = +2
Query: 200 LDYLKELGVDAAWLSPIFKSA----MHD---FGYDTTDYYTIQPEYGSMEDFEHLLKKAN 358
LDY+++LG A +SP+ + H G+ +YY + P +G+ ED + L +
Sbjct: 71 LDYIRDLGFTAISISPVVEQLEGPEYHGEAYHGHRPKNYYRLNPHFGNEEDLKELSDALH 130
Query: 359 ELNIKIVLELVPNHTSNESEWFLKSSNRDEYYSDWFIWESGHLDN 493
+ +++++ NHT SE+F D+Y+ + + ++ H+D+
Sbjct: 131 GKGMYLMVDVAINHTI--SEYF-----EDDYFKNTY-FDKTHIDH 167
>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
3|||Manual
Length = 625
Score = 38.7 bits (86), Expect = 6e-04
Identities = 28/117 (23%), Positives = 53/117 (45%), Gaps = 14/117 (11%)
Frame = +2
Query: 194 TRLDYLKELGVDAAWLSPIFKSAMHDFGYD--------TTDYYTIQPEYGSMEDFEHLLK 349
++LDY++ +G +A W+SP+ K+ +G D TD+ + +GS +D L+
Sbjct: 68 SKLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFTQLNEHFGSEDDLIDLIT 127
Query: 350 KANELNIKIVLE------LVPNHTSNESEWFLKSSNRDEYYSDWFIWESGHLDNMGI 502
+ ++ I+ + +P T N S L N Y+ + + G +N I
Sbjct: 128 DMHNRDMWIMFDALANSMAIPGPTDNISYSNLVPFNDSSYFHPYCWIDYGSNNNTDI 184
>SPAC644.04 |pct1||RNA 5'-triphosphatase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 28.7 bits (61), Expect = 0.62
Identities = 11/36 (30%), Positives = 22/36 (61%)
Frame = +2
Query: 254 KSAMHDFGYDTTDYYTIQPEYGSMEDFEHLLKKANE 361
K+ +D YDTT+ + ++ E+G++ D +KA +
Sbjct: 243 KTTQNDPVYDTTERHELEVEFGNIADLRDRAQKAKD 278
>SPBC115.03 ||SPBC839.18c|gfo/idh/mocA family oxidoreductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 368
Score = 26.6 bits (56), Expect = 2.5
Identities = 14/27 (51%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
Frame = +2
Query: 299 TIQPEYGSMED--FEHLLKKANELNIK 373
T+Q Y D FE +LKKANE IK
Sbjct: 316 TVQGNYRDFYDAVFEEILKKANEFPIK 342
>SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 5.8
Identities = 15/58 (25%), Positives = 23/58 (39%)
Frame = +2
Query: 365 NIKIVLELVPNHTSNESEWFLKSSNRDEYYSDWFIWESGHLDNMGIRKPPNNWVSVFR 538
N+ + +HT E W Y +W IWE+ L ++ + N W V R
Sbjct: 594 NLASAMLKAKDHTK-EQAWHAMQQGIKYMYDNWRIWENYMLISVDV----NKWSEVIR 646
>SPBC36.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.4 bits (53), Expect = 5.8
Identities = 15/55 (27%), Positives = 23/55 (41%)
Frame = -1
Query: 451 IFVSI**FQKPFALVASVIWDQFQNYFDIQFIGFFEKMLKILHRSIFWLYCVIIC 287
IF S FQ PF V++ W ++ N I K+L + + +I C
Sbjct: 3 IFESCHLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKVLDNGVLYTERLITC 57
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 25.0 bits (52), Expect = 7.7
Identities = 8/20 (40%), Positives = 14/20 (70%)
Frame = +3
Query: 285 PQIITQYNQNMDRWRILSIF 344
P I+TQ +++ +WR+ S F
Sbjct: 674 PNILTQVGESLKKWRLTSRF 693
>SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter
Atm1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 693
Score = 25.0 bits (52), Expect = 7.7
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +3
Query: 60 HVWELDTKM*ILSKTGGKPRCSISSIQGRSWIATAMVLEIL 182
H+ LD + +TG R +G S+I ++MVL I+
Sbjct: 205 HLLNLDMNFHLSKQTGSITRAMDRGTKGISFILSSMVLHII 245
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,541,440
Number of Sequences: 5004
Number of extensions: 52180
Number of successful extensions: 141
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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