BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte12b13
(439 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 27 0.96
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 27 1.3
SPBC18E5.14c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 1.3
SPCC1223.02 |nmt1|thi3|no message in thiamine Nmt1|Schizosacchar... 27 1.3
SPAC31G5.14 |gcv1|n313|glycine decarboxylase T subunit|Schizosac... 26 2.2
SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual 26 2.2
SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizos... 26 2.9
SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe... 25 3.9
SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 25 6.7
SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein Pop2|Schizosacchar... 25 6.7
SPCC285.03 |||ATP-dependent RNA helicase Dbp6|Schizosaccharomyce... 25 6.7
SPBC16E9.08 |mcp4|mug101|sequence orphan|Schizosaccharomyces pom... 25 6.7
SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 24 8.9
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 27.5 bits (58), Expect = 0.96
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -2
Query: 126 NFLKNTPCTFPKTSKSAVTLFTLFTAIITQTSXDFFSNL 10
N L CT P+TS V + I++Q S +F ++L
Sbjct: 478 NRLIKALCTIPRTSSFLVPYYVRLARILSQLSSEFSTSL 516
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 27.1 bits (57), Expect = 1.3
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -1
Query: 133 LRKLPEEYSLHFSKNFEIRRYTIYF 59
L EE+S HF E++R+ +Y+
Sbjct: 75 LAPFDEEFSFHFRGPIELKRFAVYY 99
>SPBC18E5.14c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 127
Score = 27.1 bits (57), Expect = 1.3
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = -1
Query: 139 FLLRKLPEEYSLHFSKNFE--IRRYTIYFVHCHHNSNFLXFF 20
++L K Y SK+F ++RY IYF +C +NF+ F
Sbjct: 28 YMLYKTYTSYFFMSSKSFVRGVKRYLIYFCYC---ANFIALF 66
>SPCC1223.02 |nmt1|thi3|no message in thiamine
Nmt1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 346
Score = 27.1 bits (57), Expect = 1.3
Identities = 14/59 (23%), Positives = 26/59 (44%), Gaps = 5/59 (8%)
Frame = +2
Query: 170 MNTAQGTVIGQLQGPSTAVD-----DMKLWLQKVGSPKSKIEKAAFRNEGNIKCCAFRT 331
MN A + G++ G ++ +++ W G P+S ++ N+ CC F T
Sbjct: 142 MNIAPAIINGEIDG-GIGIECMQQVELERWCVSQGRPRSDVQMLRIDRLANLGCCCFCT 199
>SPAC31G5.14 |gcv1|n313|glycine decarboxylase T
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 387
Score = 26.2 bits (55), Expect = 2.2
Identities = 20/66 (30%), Positives = 31/66 (46%)
Frame = +2
Query: 134 KKALELGLKGWVMNTAQGTVIGQLQGPSTAVDDMKLWLQKVGSPKSKIEKAAFRNEGNIK 313
KK +E KG + QG + +QGP TA KL + V K ++A+ + +K
Sbjct: 147 KKHIE-NWKGVELERVQGRALIAIQGPETASVVQKL-IPNVDFSVLKFGQSAYVDFKGVK 204
Query: 314 CCAFRT 331
C R+
Sbjct: 205 CLFSRS 210
>SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 723
Score = 26.2 bits (55), Expect = 2.2
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = -2
Query: 159 LSPSSNAFFCVNFLKNTPCTFPKTSKSAVTLFT-LFTAIITQTSXDFFSNLSKI 1
+S SSN FFC + TF ++ V FT LF + S F +L K+
Sbjct: 553 VSNSSNQFFCSAPKLDPTSTFSGVAQRVVNTFTNLFIPAVPTDSYFFKYHLHKV 606
>SPCC1020.10 |oca2||serine/threonine protein kinase Oca2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 650
Score = 25.8 bits (54), Expect = 2.9
Identities = 27/97 (27%), Positives = 42/97 (43%), Gaps = 7/97 (7%)
Frame = -2
Query: 327 LKAQHLIFPSFLNAAFSILDFGLPTFCNQSF----MSSTAVEG--P*S*PITVPCAVFIT 166
LK +L+ S N I+DFG F + +T V G P P T+ ++
Sbjct: 426 LKLDNLVVDS--NCFVKIIDFGSAVVFKYPFEADIVEATGVVGSDPYLAPETLVRKLYDP 483
Query: 165 HPLSPSSNAF-FCVNFLKNTPCTFPKTSKSAVTLFTL 58
+ S+A FC L+ P +PK S ++ LF +
Sbjct: 484 RAVDIWSSAIIFCCMALRRFPWKYPKLSDNSFRLFCM 520
>SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 959
Score = 25.4 bits (53), Expect = 3.9
Identities = 16/38 (42%), Positives = 18/38 (47%)
Frame = -2
Query: 159 LSPSSNAFFCVNFLKNTPCTFPKTSKSAVTLFTLFTAI 46
+S SSNAF VN L TP S V LF T +
Sbjct: 163 ISFSSNAFLAVNKLLQTPAV--SKSTEGVGLFLCLTRV 198
>SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1088
Score = 24.6 bits (51), Expect = 6.7
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = -2
Query: 261 LPTFCNQSFMSSTAVEGP*S*PITVPCAVFITHPLSPSSNAFFCVNFLKNTPCT 100
L ++ +S T ++ + PI C+ +PL +SN +F +FLKN T
Sbjct: 236 LEDILSEELVSITFLKDAVAYPIVSYCS---PNPLRETSNPYFLRDFLKNNANT 286
>SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein
Pop2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 703
Score = 24.6 bits (51), Expect = 6.7
Identities = 24/75 (32%), Positives = 40/75 (53%), Gaps = 7/75 (9%)
Frame = -1
Query: 367 FNLNIQSFTYFKC-----PKSAA--FNISLIPERRFLDLRFWASDLL*PKLHVINSSRGS 209
FN +I++ +Y KC P S F+ISL+P+R FL ++ L PK+ I+ +
Sbjct: 97 FNESIENVSY-KCLDHSPPDSVPGDFSISLVPQRNFL----YSHSSLPPKIISIDRN-NR 150
Query: 208 LKLTYNSSLRRIHYP 164
+KL + S ++P
Sbjct: 151 IKLDNSISSNSDNFP 165
>SPCC285.03 |||ATP-dependent RNA helicase Dbp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 604
Score = 24.6 bits (51), Expect = 6.7
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = -2
Query: 336 SNVLKAQHLIFPSFLNAAFSILDFGLPTFCNQSFMSSTAVEGP 208
SN+L I P+FL ++L + LP+ + S+T P
Sbjct: 323 SNILDLDQNISPTFLPDIDTLLPYRLPSPLQKLVFSATLTRDP 365
>SPBC16E9.08 |mcp4|mug101|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 24.6 bits (51), Expect = 6.7
Identities = 8/27 (29%), Positives = 16/27 (59%)
Frame = +2
Query: 122 KFTQKKALELGLKGWVMNTAQGTVIGQ 202
+F Q + L+ LKGW+ ++ + G+
Sbjct: 222 RFQQNRLLDRNLKGWIQGESEKALKGR 248
>SPCC553.09c |spb70|pol12|DNA polymerase alpha
B-subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 24.2 bits (50), Expect = 8.9
Identities = 12/35 (34%), Positives = 14/35 (40%)
Frame = -2
Query: 156 SPSSNAFFCVNFLKNTPCTFPKTSKSAVTLFTLFT 52
+PS N F L P FP +SK F T
Sbjct: 265 NPSGNMFIAKEILPIPPLPFPSSSKQEHATFVANT 299
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,712,578
Number of Sequences: 5004
Number of extensions: 33170
Number of successful extensions: 112
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 158122380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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