BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte11o19
(731 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha... 217 2e-57
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 26 4.8
SPBC14C8.11c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 26 4.8
SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb... 26 4.8
SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosa... 25 8.4
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 25 8.4
>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 217 bits (529), Expect = 2e-57
Identities = 101/169 (59%), Positives = 120/169 (71%), Gaps = 3/169 (1%)
Frame = +3
Query: 201 EKLKEGPNLTDFISEDRPKNWDE---YQGKLKREKGESERLRLPPWLKTTIPTGSKFNEI 371
EKL +GP+ DF++ D+P DE K++ G + RLP WLKT +P G+ FN I
Sbjct: 29 EKLAKGPSFADFLNMDKPLTADEAFELDRKVELPNGSIHK-RLPSWLKTKVPLGTNFNRI 87
Query: 372 KQQLRSLKLSTVCEEARCPNIGECWSGGKHGASTATIMLMGDTCTRGCMFCSVKTSRNPP 551
K LR L TVCEEA+CPNIGECW G +TATIMLMGDTCTRGC FCSVKTSR P
Sbjct: 88 KHDLRGSHLHTVCEEAKCPNIGECWGGKDKSRATATIMLMGDTCTRGCRFCSVKTSRRPG 147
Query: 552 PLDPEEPRNTAHAINQWGVGYIVLTSVDRDDLPDGGSLHFAETVKEIKK 698
PLDP EP NTA AI QW +GYIVLTSVDRDDL D G+ H A+T+++IK+
Sbjct: 148 PLDPNEPENTAEAIKQWNLGYIVLTSVDRDDLTDLGANHIAKTIQKIKE 196
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 26.2 bits (55), Expect = 4.8
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -2
Query: 691 ISLTVSAKCSEPPSGKS 641
+ + VSAK SEPPS KS
Sbjct: 60 LKMEVSAKISEPPSSKS 76
>SPBC14C8.11c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 136
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +3
Query: 570 PRNTAHAINQWGVGYIVLTSVDRDDLPDGGSLHFA 674
PR+ H N+ + Y+ T+ D LP+GG+ A
Sbjct: 15 PRSVCHVRNRENI-YLYWTTFDAHKLPNGGNADMA 48
>SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +3
Query: 246 DRPKNWDEYQGKLKREKGESERLRLPP 326
D K D Y K + + G +ER RLPP
Sbjct: 412 DAIKAVDSYSQKWQEQTGWTERFRLPP 438
>SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin
Nup132|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1162
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/56 (23%), Positives = 21/56 (37%)
Frame = -2
Query: 490 INMMVAVDAPCFPPLQHSPILGHLASSQTVLSFKLRNCCFISLNFEPVGIVVFNHG 323
I+++ P F P QH + + + KL N +N P+ V G
Sbjct: 237 IHIIALKSPPLFSPYQHLLYVAEASGLLEIYDLKLENKLVSGMNLSPIFKQVLREG 292
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 25.4 bits (53), Expect = 8.4
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +3
Query: 405 VCEEARCPNIGECWSGGKHGA 467
+C+E IGE W GKHG+
Sbjct: 1204 ICQEGE---IGEIWINGKHGS 1221
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,992,934
Number of Sequences: 5004
Number of extensions: 63480
Number of successful extensions: 224
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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