Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte11g22
(734 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10050.1 68416.m01205 threonine ammonia-lyase / threonine deh... 95 6e-20
At4g11640.1 68417.m01861 serine racemase, putative similar to se... 71 6e-13
At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme... 46 2e-05
At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme... 46 2e-05
At4g29840.2 68417.m04247 threonine synthase, chloroplast identic... 44 1e-04
At4g29840.1 68417.m04248 threonine synthase, chloroplast identic... 44 1e-04
At1g72810.1 68414.m08417 threonine synthase, putative strong sim... 44 1e-04
At5g54810.1 68418.m06827 tryptophan synthase, beta subunit 1 (TS... 35 0.049
At4g27070.1 68417.m03892 tryptophan synthase, beta subunit 2 (TS... 35 0.049
At1g52855.1 68414.m05976 expressed protein 33 0.15
At5g28237.2 68418.m03423 tryptophan synthase, beta subunit, puta... 32 0.34
At5g28237.1 68418.m03422 tryptophan synthase, beta subunit, puta... 32 0.34
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 28 5.6
At2g39030.1 68415.m04798 GCN5-related N-acetyltransferase (GNAT)... 28 5.6
At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetyls... 28 7.4
At2g03780.1 68415.m00338 translin family protein similar to SP|Q... 28 7.4
At1g20795.1 68414.m02604 F-box family protein contains Pfam prof... 27 9.8
>At3g10050.1 68416.m01205 threonine ammonia-lyase / threonine
dehydratase / threonine deaminase (OMR1) identical to
SP|Q9ZSS6 Threonine dehydratase biosynthetic,
chloroplast precursor (EC 4.3.1.19, formerly EC
4.2.1.16) (Threonine deaminase) (TD) {Arabidopsis
thaliana}
Length = 592
Score = 94.7 bits (225), Expect = 6e-20
Identities = 50/143 (34%), Positives = 79/143 (55%), Gaps = 1/143 (0%)
Frame = +1
Query: 187 SRRIVGAVVRTPCTRAH-MSERLGMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKKNG 363
S ++ + +P A +S+RLG+ +Y K+E LQ SFK RG N ++ L +Q G
Sbjct: 101 STKVYDIAIESPLQLAKKLSKRLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKG 160
Query: 364 VIAASTGNHGAALSYHSTQLGIPCIVVVPIHTALNKVNKCEQLGAKVFKHGIDMSAAKLH 543
VI +S GNH ++ +++LG ++V+P+ T K E LGA V G A+ H
Sbjct: 161 VICSSAGNHAQGVALSASKLGCTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAH 220
Query: 544 AMSLGKEKKMIYINGYDHPDVLA 612
A +E+ + +I +DHPDV+A
Sbjct: 221 AKIRAEEEGLTFIPPFDHPDVIA 243
>At4g11640.1 68417.m01861 serine racemase, putative similar to
serine racemase [Mus musculus] GI:6448865; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 346
Score = 71.3 bits (167), Expect = 6e-13
Identities = 41/129 (31%), Positives = 66/129 (51%)
Frame = +1
Query: 292 TGSFKERGVRNALISLSDEQKKNGVIAASTGNHGAALSYHSTQLGIPCIVVVPIHTALNK 471
+G+FK RG NA++SL EQ GV+ S+GNH AALS + GIP +VVP K
Sbjct: 70 SGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHAAALSLAAKIQGIPAYIVVPKGAPKCK 129
Query: 472 VNKCEQLGAKVFKHGIDMSAAKLHAMSLGKEKKMIYINGYDHPDVLAXXXXXXXXXXXXL 651
V+ + G KV MS+ + A + +E + I+ Y+ +++ +
Sbjct: 130 VDNVIRYGGKVIWSEATMSSREEIASKVLQETGSVLIHPYNDGRIISGQGTIALELLEQI 189
Query: 652 PNVDAVLIP 678
+DA+++P
Sbjct: 190 QEIDAIVVP 198
>At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 348
Score = 46.4 bits (105), Expect = 2e-05
Identities = 40/121 (33%), Positives = 57/121 (47%), Gaps = 5/121 (4%)
Frame = +1
Query: 157 KIKYDDILAASRRIVGAVVRTPCTRAH-MSERLGMDIYFKQEFLQYTGSFKERGVRNALI 333
K K + L +V A+ TP R + +SE G +I K EFL GS K+R V +I
Sbjct: 31 KKKRKEKLTMRNGLVDAIGNTPLIRINSLSEATGCEILGKCEFLNPGGSVKDR-VAVKII 89
Query: 334 SLSDEQKK---NGVIA-ASTGNHGAALSYHSTQLGIPCIVVVPIHTALNKVNKCEQLGAK 501
+ E K G++ S G+ +L+ + G C VV+P A+ K E LGA
Sbjct: 90 QEALESGKLFPGGIVTEGSAGSTAISLATVAPAYGCKCHVVIPDDAAIEKSQIIEALGAS 149
Query: 502 V 504
V
Sbjct: 150 V 150
>At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 421
Score = 46.4 bits (105), Expect = 2e-05
Identities = 40/121 (33%), Positives = 57/121 (47%), Gaps = 5/121 (4%)
Frame = +1
Query: 157 KIKYDDILAASRRIVGAVVRTPCTRAH-MSERLGMDIYFKQEFLQYTGSFKERGVRNALI 333
K K + L +V A+ TP R + +SE G +I K EFL GS K+R V +I
Sbjct: 31 KKKRKEKLTMRNGLVDAIGNTPLIRINSLSEATGCEILGKCEFLNPGGSVKDR-VAVKII 89
Query: 334 SLSDEQKK---NGVIA-ASTGNHGAALSYHSTQLGIPCIVVVPIHTALNKVNKCEQLGAK 501
+ E K G++ S G+ +L+ + G C VV+P A+ K E LGA
Sbjct: 90 QEALESGKLFPGGIVTEGSAGSTAISLATVAPAYGCKCHVVIPDDAAIEKSQIIEALGAS 149
Query: 502 V 504
V
Sbjct: 150 V 150
>At4g29840.2 68417.m04247 threonine synthase, chloroplast identical
to SP|Q9S7B5 Threonine synthase, chloroplast precursor
(EC 4.2.3.1) (TS) {Arabidopsis thaliana}
Length = 535
Score = 43.6 bits (98), Expect = 1e-04
Identities = 30/73 (41%), Positives = 42/73 (57%), Gaps = 6/73 (8%)
Frame = +1
Query: 250 LGM-DIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN-----GVIAASTGNHGAALSYH 411
LGM D++ K + +TGSFK+ G+ L+S + +K GV ASTG+ AALS +
Sbjct: 184 LGMNDLWVKHCGISHTGSFKDLGM-TVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAY 242
Query: 412 STQLGIPCIVVVP 450
GIP IV +P
Sbjct: 243 CASAGIPSIVFLP 255
>At4g29840.1 68417.m04248 threonine synthase, chloroplast identical
to SP|Q9S7B5 Threonine synthase, chloroplast precursor
(EC 4.2.3.1) (TS) {Arabidopsis thaliana}
Length = 526
Score = 43.6 bits (98), Expect = 1e-04
Identities = 30/73 (41%), Positives = 42/73 (57%), Gaps = 6/73 (8%)
Frame = +1
Query: 250 LGM-DIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN-----GVIAASTGNHGAALSYH 411
LGM D++ K + +TGSFK+ G+ L+S + +K GV ASTG+ AALS +
Sbjct: 184 LGMNDLWVKHCGISHTGSFKDLGM-TVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAY 242
Query: 412 STQLGIPCIVVVP 450
GIP IV +P
Sbjct: 243 CASAGIPSIVFLP 255
>At1g72810.1 68414.m08417 threonine synthase, putative strong
similarity to SP|Q9S7B5 Threonine synthase, chloroplast
precursor (EC 4.2.3.1) (TS) {Arabidopsis thaliana}
Length = 516
Score = 43.6 bits (98), Expect = 1e-04
Identities = 26/68 (38%), Positives = 39/68 (57%), Gaps = 4/68 (5%)
Frame = +1
Query: 259 DIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN----GVIAASTGNHGAALSYHSTQLG 426
D++ K + +TGSFK+ G+ + ++ +K N GV ASTG+ AALS + G
Sbjct: 179 DLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAG 238
Query: 427 IPCIVVVP 450
IP IV +P
Sbjct: 239 IPSIVFLP 246
>At5g54810.1 68418.m06827 tryptophan synthase, beta subunit 1 (TSB1)
identical to SP|P14671
Length = 470
Score = 35.1 bits (77), Expect = 0.049
Identities = 31/98 (31%), Positives = 45/98 (45%), Gaps = 5/98 (5%)
Frame = +1
Query: 262 IYFKQEFLQYTGSFK-ERGVRNALISLSDEQKKNGVIAASTGNHGAALSYHSTQLGIPCI 438
IY K+E L +TG+ K V AL++ KK + G HG A + + G+ CI
Sbjct: 151 IYLKREDLNHTGAHKINNAVAQALLA-KRLGKKRIIAETGAGQHGVATATVCARFGLECI 209
Query: 439 VVVPI----HTALNKVNKCEQLGAKVFKHGIDMSAAKL 540
+ + ALN V + LGA+V G+ A L
Sbjct: 210 IYMGAQDMERQALN-VFRMRLLGAEV--RGVHSGTATL 244
>At4g27070.1 68417.m03892 tryptophan synthase, beta subunit 2 (TSB2)
identical to SP|25269
Length = 475
Score = 35.1 bits (77), Expect = 0.049
Identities = 31/98 (31%), Positives = 45/98 (45%), Gaps = 5/98 (5%)
Frame = +1
Query: 262 IYFKQEFLQYTGSFK-ERGVRNALISLSDEQKKNGVIAASTGNHGAALSYHSTQLGIPCI 438
IY K+E L +TG+ K V AL++ KK + G HG A + + G+ CI
Sbjct: 156 IYLKREDLNHTGAHKINNAVAQALLA-KRLGKKRIIAETGAGQHGVATATVCARFGLQCI 214
Query: 439 VVVPI----HTALNKVNKCEQLGAKVFKHGIDMSAAKL 540
+ + ALN V + LGA+V G+ A L
Sbjct: 215 IYMGAQDMERQALN-VFRMRLLGAEV--RGVHSGTATL 249
>At1g52855.1 68414.m05976 expressed protein
Length = 68
Score = 33.5 bits (73), Expect = 0.15
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = +3
Query: 324 CADIVE*RTKEKWCYSSVYWKSWCSIKLS 410
CA I + +T ++C+ +YW+SW ++ S
Sbjct: 30 CASIADMKTSTRFCFIPIYWRSWRAVVCS 58
>At5g28237.2 68418.m03423 tryptophan synthase, beta subunit,
putative similar to SP|P14671 Tryptophan synthase beta
chain 1, chloroplast precursor (EC 4.2.1.20)
{Arabidopsis thaliana}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 372
Score = 32.3 bits (70), Expect = 0.34
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Frame = +1
Query: 253 GMDIYFKQEFLQYTGSFKERGVRNALIS--LSDEQKKNGVIAAS-TGNHGAALSYHSTQL 423
G +IY K+E L + GS K + NAL +S + V+AA+ G HG A + +L
Sbjct: 142 GPEIYLKREDLSHCGSHK---INNALAQAMISRRLGCSRVVAATGAGQHGVATAAACAKL 198
Query: 424 GIPCIV 441
+ C V
Sbjct: 199 SLECTV 204
>At5g28237.1 68418.m03422 tryptophan synthase, beta subunit,
putative similar to SP|P14671 Tryptophan synthase beta
chain 1, chloroplast precursor (EC 4.2.1.20)
{Arabidopsis thaliana}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 465
Score = 32.3 bits (70), Expect = 0.34
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Frame = +1
Query: 253 GMDIYFKQEFLQYTGSFKERGVRNALIS--LSDEQKKNGVIAAS-TGNHGAALSYHSTQL 423
G +IY K+E L + GS K + NAL +S + V+AA+ G HG A + +L
Sbjct: 142 GPEIYLKREDLSHCGSHK---INNALAQAMISRRLGCSRVVAATGAGQHGVATAAACAKL 198
Query: 424 GIPCIV 441
+ C V
Sbjct: 199 SLECTV 204
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -3
Query: 171 IIFYFAWIIWITVFIKFNIDTHFD 100
+IF FA+ IW+ VF NI T D
Sbjct: 31 LIFVFAFTIWVLVFSSRNIQTDDD 54
>At2g39030.1 68415.m04798 GCN5-related N-acetyltransferase (GNAT)
family protein similar to SP|Q9SMB8 Tyramine
N-feruloyltransferase 4/11 (EC 2.3.1.110)
(Hydroxycinnamoyl- CoA: tyramine
N-hydroxycinnamoyltransferase) {Nicotiana tabacum};
contains Pfam profile PF00583: acetyltransferase, GNAT
family
Length = 228
Score = 28.3 bits (60), Expect = 5.6
Identities = 13/38 (34%), Positives = 24/38 (63%)
Frame = +1
Query: 397 ALSYHSTQLGIPCIVVVPIHTALNKVNKCEQLGAKVFK 510
A++ + +LG+ + + I +N +N EQ+GA+VFK
Sbjct: 171 AVAKQAVKLGVGRVEWIVIDWNVNAINFYEQMGAQVFK 208
>At3g04940.1 68416.m00536 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 324
Score = 27.9 bits (59), Expect = 7.4
Identities = 21/82 (25%), Positives = 37/82 (45%), Gaps = 4/82 (4%)
Frame = +1
Query: 271 KQEFLQYTGSFKERGVRNALISLSDE----QKKNGVIAASTGNHGAALSYHSTQLGIPCI 438
K E ++ S KER + D+ K+ +I A++GN G L++ G +
Sbjct: 38 KLEMMEPCSSVKERIAYGMIKDAEDKGLITPGKSTLIEATSGNTGIGLAFIGAAKGYKVV 97
Query: 439 VVVPIHTALNKVNKCEQLGAKV 504
+ +P +L + LGA+V
Sbjct: 98 LTMPSSMSLERKIILLALGAEV 119
>At2g03780.1 68415.m00338 translin family protein similar to
SP|Q99598 Translin-associated protein X
(Translin-associated factor X) {Homo sapiens}; contains
Pfam profile PF01997: Translin family
Length = 287
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 285 TVYWKFQRTWSPKCADIVE*RTKEKWCYS 371
T +WK +R +SP + VE T K+C S
Sbjct: 126 TDFWKLRRAYSPGVQEYVEAATFYKFCLS 154
>At1g20795.1 68414.m02604 F-box family protein contains Pfam
profile: PF00646 F-box domain
Length = 418
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/52 (30%), Positives = 25/52 (48%)
Frame = +1
Query: 205 AVVRTPCTRAHMSERLGMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN 360
++V CTR + + D YFK ++L GVR+ L+ +S KN
Sbjct: 22 SLVMMKCTRRSFNSHISEDPYFKSKYLS--------GVRSGLLHISSFGSKN 65
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,545,692
Number of Sequences: 28952
Number of extensions: 322739
Number of successful extensions: 783
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 758
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 776
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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