Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte11e24
(725 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g54440.1 68416.m06023 glycoside hydrolase family 2 protein si... 61 8e-10
At5g66460.1 68418.m08381 (1-4)-beta-mannan endohydrolase, putati... 37 0.012
At5g01930.1 68418.m00112 (1-4)-beta-mannan endohydrolase, putati... 34 0.083
At4g28320.1 68417.m04055 glycosyl hydrolase family 5 protein / c... 33 0.15
At2g20680.1 68415.m02428 glycosyl hydrolase family 5 protein / c... 33 0.19
At1g02310.1 68414.m00176 glycosyl hydrolase family protein 5 / c... 32 0.34
At1g80810.1 68414.m09481 expressed protein similar to androgen-i... 31 1.0
At3g10890.1 68416.m01311 (1-4)-beta-mannan endohydrolase, putati... 30 1.4
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 30 1.8
At3g45890.1 68416.m04966 expressed protein contains Pfam domain,... 29 2.4
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 29 2.4
At3g56610.1 68416.m06295 expressed protein 29 3.1
At2g31600.2 68415.m03861 expressed protein 28 7.2
At2g31600.1 68415.m03860 expressed protein 28 7.2
At1g03360.1 68414.m00315 exonuclease family protein similar to ... 28 7.2
At5g54210.1 68418.m06753 NLI interacting factor (NIF) family pro... 27 9.6
At4g01090.1 68417.m00147 extra-large G-protein-related contains ... 27 9.6
At3g61590.1 68416.m06898 F-box family protein contains weak hit ... 27 9.6
At3g10900.1 68416.m01312 (1-4)-beta-mannan endohydrolase, putati... 27 9.6
At2g01470.1 68415.m00070 St12p protein (ST12p) / SEC12p protein,... 27 9.6
>At3g54440.1 68416.m06023 glycoside hydrolase family 2 protein
similar to beta-galactosidase (lactase) from Alteromonas
haloplanktis [SP|P81650]; contains Pfam glycoside
hydrolase domains PF02836, PF02837, PF02929, PF02930
Length = 1107
Score = 60.9 bits (141), Expect = 8e-10
Identities = 39/124 (31%), Positives = 58/124 (46%), Gaps = 11/124 (8%)
Frame = +1
Query: 19 IYLNSKPLYLRGFGMHEDSDLRGKGWDPVLWVKNFNLIKWIGANAFRTSHYPYAEEIYQL 198
+ +N P+ ++G HE GK VK+ ++K NA R SHYP Y+L
Sbjct: 399 LLVNGHPVVIKGVNRHEHHPRVGKTNIEACMVKDLIMMKEYNINAVRNSHYPQHPRWYEL 458
Query: 199 ADEYGIMIIDECPSGDTDIFTQSLLNKHK-----------QSLTELIRRDKNHPSVIMWS 345
D +G+ +IDE + +T F S KH + ++ RDKNH +I WS
Sbjct: 459 CDLFGMYMIDEA-NIETHGFDLSGHLKHPAKEPSWAAAMLDRVVGMVERDKNHTCIISWS 517
Query: 346 ISNE 357
+ NE
Sbjct: 518 LGNE 521
>At5g66460.1 68418.m08381 (1-4)-beta-mannan endohydrolase, putative
similar to (1-4)-beta-mannan endohydrolase [Coffea
arabica] GI:10178872; contains Pfam profile PF00150:
Cellulase (glycosyl hydrolase family 5)
Length = 431
Score = 37.1 bits (82), Expect = 0.012
Identities = 23/89 (25%), Positives = 42/89 (47%), Gaps = 13/89 (14%)
Frame = +1
Query: 238 SGDTDIFTQSLLNK-HKQSLTELIRRD--------KNHPSVIMWSISNEPRSAKQQA--- 381
S + D FT SL+ +K + ++ R K+ P+++ W + NEPR +
Sbjct: 155 SSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDPSGRA 214
Query: 382 -DAYFAEVVKHVKSMDLSRPVTIAISQYY 465
A+ E+ HVKS+D + + + +Y
Sbjct: 215 IQAWITEMAAHVKSLDRNHLLEAGLEGFY 243
>At5g01930.1 68418.m00112 (1-4)-beta-mannan endohydrolase, putative
similar to (1-4)-beta-mannan endohydrolase [Coffea
arabica] GI:10178872; contains Pfam profile PF00150:
Cellulase (glycosyl hydrolase family 5)
Length = 448
Score = 34.3 bits (75), Expect = 0.083
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 4/54 (7%)
Frame = +1
Query: 316 KNHPSVIMWSISNEPR----SAKQQADAYFAEVVKHVKSMDLSRPVTIAISQYY 465
KN P++ W + NEPR + + ++ E+ VKS+D V I + +Y
Sbjct: 206 KNDPTIFAWELMNEPRCPSDPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFY 259
>At4g28320.1 68417.m04055 glycosyl hydrolase family 5 protein /
cellulase family protein mannan
endo-1,4-beta-mannosidase (EC 3.2.1.78) - tomato,
PIR2:T04323
Length = 431
Score = 33.5 bits (73), Expect = 0.15
Identities = 25/89 (28%), Positives = 40/89 (44%), Gaps = 13/89 (14%)
Frame = +1
Query: 238 SGDTDIFTQSLLNKHKQSLTELIRRD--------KNHPSVIMWSISNEPR-----SAKQQ 378
S D+ F S+ N K L L+ R +N P++ W + NEPR S K
Sbjct: 167 SNDSFFFDPSIRNYFKNYLKVLLTRKNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTL 226
Query: 379 ADAYFAEVVKHVKSMDLSRPVTIAISQYY 465
D + E+ +KS+D +T+ + +Y
Sbjct: 227 QD-WIDEMTGFIKSIDDKHLLTVGLEGFY 254
>At2g20680.1 68415.m02428 glycosyl hydrolase family 5 protein /
cellulase family protein similar to (1-4)-beta-mannan
endohydrolase [Coffea arabica] GI:10178872; contains
Pfam profile PF00150: Cellulase (glycosyl hydrolase
family 5)
Length = 433
Score = 33.1 bits (72), Expect = 0.19
Identities = 22/88 (25%), Positives = 38/88 (43%), Gaps = 12/88 (13%)
Frame = +1
Query: 238 SGDTDIFTQSLLNKHKQSLTELIRRD--------KNHPSVIMWSISNEPRSAKQQA---- 381
S D+ F S+ K LT L+ R +N P++ W + NEPR +
Sbjct: 168 SNDSFFFDPSIRRYFKNYLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTL 227
Query: 382 DAYFAEVVKHVKSMDLSRPVTIAISQYY 465
+ E+ +KS+D +T+ + +Y
Sbjct: 228 QDWINEMTAFIKSIDNKHLLTVGLEGFY 255
>At1g02310.1 68414.m00176 glycosyl hydrolase family protein 5 /
cellulase family protein / (1-4)-beta-mannan
endohydrolase, putative similar to (1-4)-beta-mannan
endohydrolase precursor GI:9836826 from [Lycopersicon
esculentum]
Length = 411
Score = 32.3 bits (70), Expect = 0.34
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Frame = +1
Query: 307 RRDKNHPSVIMWSISNEPR----SAKQQADAYFAEVVKHVKSMDLSRPVTIAISQYY 465
R K+ P++ W + NEPR +A + E+ +VKS+D + + I + +Y
Sbjct: 182 RMYKDDPTIFSWELINEPRCNDSTASNILQDWVKEMASYVKSIDSNHLLEIGLEGFY 238
>At1g80810.1 68414.m09481 expressed protein similar to
androgen-induced prostate proliferative shutoff
associated protein (GI:4559410) [Homo sapiens]; similar
to bimD (GI:168025) [Emericella nidulans]
Length = 826
Score = 30.7 bits (66), Expect = 1.0
Identities = 28/122 (22%), Positives = 55/122 (45%), Gaps = 3/122 (2%)
Frame = +1
Query: 121 FNLIKWIGANAFRTSHYPYAEEIYQLADEYGIMIIDECPSGDTDIFTQSLLNKHKQSLTE 300
++LI + N F+ + + ++ + I IIDE TD+ SL
Sbjct: 150 YDLILQMFRNFFKFIRSDHPQLVFSSMELIMIAIIDETEQVSTDLL---------DSLLA 200
Query: 301 LIRRDKNHPSVIMWSISNEPRS-AKQQADAYFAEVVK-HVKSMDLSRPVTIAISQ-YYNT 471
++++ + S + WS++ + S ++ Y E +K S+D+ PV +I Q +NT
Sbjct: 201 TVKKENQNVSPMSWSLAEKVLSRCARKLKPYIIEALKSRGTSLDMYSPVVSSICQSVFNT 260
Query: 472 DR 477
+
Sbjct: 261 PK 262
>At3g10890.1 68416.m01311 (1-4)-beta-mannan endohydrolase, putative
similar to (1-4)-beta-mannan endohydrolase [Coffea
arabica] GI:10178872, (1-4)-beta-mannan endohydrolase
GB:AAB87859 [Lycopersicon esculentum]; contains Pfam
profile PF00150: Cellulase (glycosyl hydrolase family 5)
Length = 414
Score = 30.3 bits (65), Expect = 1.4
Identities = 20/89 (22%), Positives = 43/89 (48%), Gaps = 13/89 (14%)
Frame = +1
Query: 238 SGDTDIFTQSLLNKH-KQSLTELIRRD--------KNHPSVIMWSISNEPR----SAKQQ 378
S D D +T L+ ++ K + ++ R K+ P+++ W + NEP+ + +
Sbjct: 155 SSDDDFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKT 214
Query: 379 ADAYFAEVVKHVKSMDLSRPVTIAISQYY 465
A+ E+ +VKS+D ++ + +Y
Sbjct: 215 LTAWMNEMALYVKSVDSKHLLSTGLEGFY 243
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 29.9 bits (64), Expect = 1.8
Identities = 21/86 (24%), Positives = 38/86 (44%), Gaps = 4/86 (4%)
Frame = +1
Query: 220 IIDECPSGDTDIFTQSLLNKHKQSLTELIRRDKNHPSVIMWSIS---NEPRSAK-QQADA 387
I+D D + + KH++ L +R++ PS++ S S PR A
Sbjct: 3222 IVDSQKKIDGSVTFSKFVEKHRRLLNSFVRQN---PSLLEKSFSMMLKAPRLIDFDNKKA 3278
Query: 388 YFAEVVKHVKSMDLSRPVTIAISQYY 465
YF ++H +S P+ I++ + Y
Sbjct: 3279 YFRSRIRHQHDQHISGPLRISVRRAY 3304
>At3g45890.1 68416.m04966 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647
Length = 608
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +3
Query: 42 ISEGIWDARRLGSQRQRMGPSFVGEEF 122
+ E +W+ R GS+R+R+ P FV +EF
Sbjct: 144 VKETVWEVR--GSKRKRLVPDFVKDEF 168
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 29.5 bits (63), Expect = 2.4
Identities = 21/85 (24%), Positives = 37/85 (43%), Gaps = 4/85 (4%)
Frame = +1
Query: 223 IDECPSGDTDIFTQSLLNKHKQSLTELIRRDKNHPSVIMWSIS---NEPRSAK-QQADAY 390
+D D + KH++ L IR++ PS++ S+S PR AY
Sbjct: 3456 VDSQKKTDGSVTFSKFAEKHRRLLNSFIRQN---PSLLEKSLSMMLKAPRLIDFDNKKAY 3512
Query: 391 FAEVVKHVKSMDLSRPVTIAISQYY 465
F ++H +S P+ I++ + Y
Sbjct: 3513 FRSRIRHQHDQHISGPLRISVRRAY 3537
>At3g56610.1 68416.m06295 expressed protein
Length = 128
Score = 29.1 bits (62), Expect = 3.1
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 318 ESPERHHVVHLQRAAVCQTTGRCLFR*SSETREVHGPLQTRHH 446
+SPE H+V+ + + + RC + +HGP++ +HH
Sbjct: 34 QSPESDHLVN---KCMAKLSSRCAMYVTDAICTIHGPMRLKHH 73
>At2g31600.2 68415.m03861 expressed protein
Length = 216
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 299 SSSDATRITRASSCGPSPTSRGLPNNRPMPISL 397
+S+ A + R S+ P+P++ GLP+ PI++
Sbjct: 33 ASTSAEPLIRNSTLNPNPSTSGLPSTSNSPITM 65
>At2g31600.1 68415.m03860 expressed protein
Length = 301
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 299 SSSDATRITRASSCGPSPTSRGLPNNRPMPISL 397
+S+ A + R S+ P+P++ GLP+ PI++
Sbjct: 33 ASTSAEPLIRNSTLNPNPSTSGLPSTSNSPITM 65
>At1g03360.1 68414.m00315 exonuclease family protein similar to
Exosome complex exonuclease RRP4 (Ribosomal RNA
processing protein 4)(SP:P38792) {Saccharomyces
cerevisiae}
Length = 322
Score = 27.9 bits (59), Expect = 7.2
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -3
Query: 414 HVFHYFSEIGIGLLFGRPRLVGDGPHDDARVILVASDE 301
H FHY +GI L+ G + G H + R + D+
Sbjct: 197 HHFHYVESLGIDLIIGCNGFIWVGEHVEVRDPMAIDDQ 234
>At5g54210.1 68418.m06753 NLI interacting factor (NIF) family
protein contains Pfam profile PF03031: NLI interacting
factor
Length = 306
Score = 27.5 bits (58), Expect = 9.6
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +1
Query: 136 WIGANAFRTSHYPYAEEI-YQLADEYGIMIIDECPSGDTDIFTQSLLNKHKQSLTELIR- 309
+ G + PY + + LADE G++I+D+ P D HK++L E+ +
Sbjct: 181 YFGDRVITRNESPYIKTLDLVLADECGVVIVDDTPHVWPD---------HKRNLLEITKY 231
Query: 310 ---RDKNHPSVIMWSISNEPRSAKQQADAYFAEVVKHVKSM 423
DK V E + + + D A V+K +K +
Sbjct: 232 NYFSDKTRHDVKYTKSYAEEKRDESRNDGSLANVLKVIKQV 272
>At4g01090.1 68417.m00147 extra-large G-protein-related contains
weak similarity to extra-large G-protein [Arabidopsis
thaliana] gi|3201682|gb|AAC19353
Length = 714
Score = 27.5 bits (58), Expect = 9.6
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = +1
Query: 202 DEYGIMIIDECPSGDTDIFTQSLLNKHKQSLTELI----RRDKNHPSVI 336
+E+ ++D C +G+TD+F HK+ L+ RDKN ++
Sbjct: 625 EEFSHPMLDNCAAGNTDVFVNG-RELHKRDFELLVGRGLPRDKNRSYIV 672
>At3g61590.1 68416.m06898 F-box family protein contains weak hit to
Pfam PF00646: F-box domain; stamina pistilloidia (Stp) -
Pisum sativum, EMBL:AF004843
Length = 411
Score = 27.5 bits (58), Expect = 9.6
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = -1
Query: 623 SVMMTGLLCRRWNSVASSTTLL 558
S+ G +C+RWN + SS L
Sbjct: 59 SIFRAGTVCKRWNEIVSSRRFL 80
>At3g10900.1 68416.m01312 (1-4)-beta-mannan endohydrolase, putative
similar to (1-4)-beta-mannan endohydrolase [Coffea
arabica] GI:10178872, (1-4)-beta-mannan endohydrolase
GB:AAB87859 [Lycopersicon esculentum]; contains Pfam
profile PF00150: Cellulase (glycosyl hydrolase family 5)
Length = 408
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +1
Query: 316 KNHPSVIMWSISNEPR-----SAKQQADAYFAEVVKHVKSMDLSRPVTIAISQYY 465
K+ P+++ W + NEPR S K D + E+ VKS+D + ++ +Y
Sbjct: 189 KDEPAIMAWQLMNEPRCGVDKSGKTLMD-WINEMAPFVKSVDPNHLLSTGHEGFY 242
>At2g01470.1 68415.m00070 St12p protein (ST12p) / SEC12p protein,
putative 99.8% identical to St12p protein (GI:166878)
{Arabidopsis thaliana}
Length = 393
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = -1
Query: 692 TWYSSDHTYSGIKCRPATVSAPYSVMMTGLLCRRWNSVASS 570
T + +S +KC + S + V + G +CR W+ AS+
Sbjct: 187 TLLNESQAHSSVKCLTFSESGQFLVSLGGPVCRVWDVNASA 227
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,382,379
Number of Sequences: 28952
Number of extensions: 341772
Number of successful extensions: 1007
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 973
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1004
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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