Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte10p15
(709 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04520.1 68416.m00479 threonine aldolase family protein simil... 179 1e-45
At1g08630.3 68414.m00958 L-allo-threonine aldolase-related simil... 167 5e-42
At1g08630.2 68414.m00957 L-allo-threonine aldolase-related simil... 167 5e-42
At1g08630.1 68414.m00956 L-allo-threonine aldolase-related simil... 167 5e-42
At1g33700.1 68414.m04167 expressed protein contains Pfam domain ... 31 0.75
At4g10060.1 68417.m01645 expressed protein contains Pfam domain ... 31 0.99
At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloropla... 29 4.0
At3g13222.1 68416.m01655 expressed protein 28 5.3
At1g64660.1 68414.m07330 Cys/Met metabolism pyridoxal-phosphate-... 28 5.3
>At3g04520.1 68416.m00479 threonine aldolase family protein similar
to L-allo-threonine aldolase SP:O07051 from [Aeromonas
jandaei]
Length = 355
Score = 179 bits (436), Expect = 1e-45
Identities = 81/155 (52%), Positives = 113/155 (72%), Gaps = 2/155 (1%)
Frame = +2
Query: 251 VDLRSDTVTKPTKAMKHAMVNSALGDDVYGEDPTVNALENKIAALLGKQAALFVPSGTMS 430
VDLRSDTVTKPT++M+ AM N+ + DDV G DPT LE ++A + GK+AA+FVPSGTM
Sbjct: 10 VDLRSDTVTKPTESMRSAMANAEVDDDVLGNDPTALRLEKEVAEIAGKEAAMFVPSGTMG 69
Query: 431 NLIAIMVHCNKRGSEAFVGDLSHIYKYEVGGASHLAGVMLSTVANKPDGTFDLQELEDRI 610
NLI+++VHC++RGSE +GD SHI+ YE GG S L GV TV N+ DGT ++ +E +
Sbjct: 70 NLISVLVHCDERGSEVILGDDSHIHIYENGGVSSLGGVHPRTVKNEEDGTMEIGAIEAAV 129
Query: 611 RG--SNIHEPVTAMVAVENTHNYCGGKVLPLKFLD 709
R ++H PVT ++ +ENT CGG+ LP++++D
Sbjct: 130 RSPKGDLHHPVTKLICLENTQANCGGRCLPIEYID 164
>At1g08630.3 68414.m00958 L-allo-threonine aldolase-related similar
to L-allo-threonine aldolase (EC 4.1.2.-) (L-allo-TA)
(L-allo-threonine acetaldehyde-lyase) (SP:O07051)
{Aeromonas jandaei}; similar to ESTs gb|R30517,
gb|T42772, gb|R90493, and gb|R90493
Length = 358
Score = 167 bits (407), Expect = 5e-42
Identities = 82/156 (52%), Positives = 109/156 (69%), Gaps = 3/156 (1%)
Frame = +2
Query: 251 VDLRSDTVTKPTKAMKHAMVNSALGDDVYGEDPTVNALENKIAALLGKQAALFVPSGTMS 430
VDLRSDTVT+PT AM+ AM N+ + DDV G DPT LE ++A ++GK+AALFVPSGTM
Sbjct: 6 VDLRSDTVTRPTDAMREAMCNAEVDDDVLGYDPTARRLEEEMAKMMGKEAALFVPSGTMG 65
Query: 431 NLIAIMVHCNKRGSEAFVGDLSHIYKYEVGGASHLAGVMLSTVANKPDGTFDLQELEDRI 610
NLI++MVHC+ RGSE +GD HI+ YE GG S + GV TV N+ DGT DL+ +E I
Sbjct: 66 NLISVMVHCDVRGSEVILGDNCHIHVYENGGISTIGGVHPKTVKNEEDGTMDLEAIEAAI 125
Query: 611 R---GSNIHEPVTAMVAVENTHNYCGGKVLPLKFLD 709
R GS + P T ++ +ENTH GG+ L +++ +
Sbjct: 126 RDPKGSTFY-PSTRLICLENTHANSGGRCLSVEYTE 160
>At1g08630.2 68414.m00957 L-allo-threonine aldolase-related similar
to L-allo-threonine aldolase (EC 4.1.2.-) (L-allo-TA)
(L-allo-threonine acetaldehyde-lyase) (SP:O07051)
{Aeromonas jandaei}; similar to ESTs gb|R30517,
gb|T42772, gb|R90493, and gb|R90493
Length = 358
Score = 167 bits (407), Expect = 5e-42
Identities = 82/156 (52%), Positives = 109/156 (69%), Gaps = 3/156 (1%)
Frame = +2
Query: 251 VDLRSDTVTKPTKAMKHAMVNSALGDDVYGEDPTVNALENKIAALLGKQAALFVPSGTMS 430
VDLRSDTVT+PT AM+ AM N+ + DDV G DPT LE ++A ++GK+AALFVPSGTM
Sbjct: 6 VDLRSDTVTRPTDAMREAMCNAEVDDDVLGYDPTARRLEEEMAKMMGKEAALFVPSGTMG 65
Query: 431 NLIAIMVHCNKRGSEAFVGDLSHIYKYEVGGASHLAGVMLSTVANKPDGTFDLQELEDRI 610
NLI++MVHC+ RGSE +GD HI+ YE GG S + GV TV N+ DGT DL+ +E I
Sbjct: 66 NLISVMVHCDVRGSEVILGDNCHIHVYENGGISTIGGVHPKTVKNEEDGTMDLEAIEAAI 125
Query: 611 R---GSNIHEPVTAMVAVENTHNYCGGKVLPLKFLD 709
R GS + P T ++ +ENTH GG+ L +++ +
Sbjct: 126 RDPKGSTFY-PSTRLICLENTHANSGGRCLSVEYTE 160
>At1g08630.1 68414.m00956 L-allo-threonine aldolase-related similar
to L-allo-threonine aldolase (EC 4.1.2.-) (L-allo-TA)
(L-allo-threonine acetaldehyde-lyase) (SP:O07051)
{Aeromonas jandaei}; similar to ESTs gb|R30517,
gb|T42772, gb|R90493, and gb|R90493
Length = 358
Score = 167 bits (407), Expect = 5e-42
Identities = 82/156 (52%), Positives = 109/156 (69%), Gaps = 3/156 (1%)
Frame = +2
Query: 251 VDLRSDTVTKPTKAMKHAMVNSALGDDVYGEDPTVNALENKIAALLGKQAALFVPSGTMS 430
VDLRSDTVT+PT AM+ AM N+ + DDV G DPT LE ++A ++GK+AALFVPSGTM
Sbjct: 6 VDLRSDTVTRPTDAMREAMCNAEVDDDVLGYDPTARRLEEEMAKMMGKEAALFVPSGTMG 65
Query: 431 NLIAIMVHCNKRGSEAFVGDLSHIYKYEVGGASHLAGVMLSTVANKPDGTFDLQELEDRI 610
NLI++MVHC+ RGSE +GD HI+ YE GG S + GV TV N+ DGT DL+ +E I
Sbjct: 66 NLISVMVHCDVRGSEVILGDNCHIHVYENGGISTIGGVHPKTVKNEEDGTMDLEAIEAAI 125
Query: 611 R---GSNIHEPVTAMVAVENTHNYCGGKVLPLKFLD 709
R GS + P T ++ +ENTH GG+ L +++ +
Sbjct: 126 RDPKGSTFY-PSTRLICLENTHANSGGRCLSVEYTE 160
>At1g33700.1 68414.m04167 expressed protein contains Pfam domain
PF04685: Protein of unknown function, DUF608
Length = 947
Score = 31.1 bits (67), Expect = 0.75
Identities = 22/99 (22%), Positives = 45/99 (45%), Gaps = 3/99 (3%)
Frame = +2
Query: 383 LLGKQAALFVPSGTMSNLIAIMVH---CNKRGSEAFVGDLSHIYKYEVGGASHLAGVMLS 553
++ +Q + F+P + + + V G+E + L ++ VGGAS L G +
Sbjct: 197 IVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQHFN 256
Query: 554 TVANKPDGTFDLQELEDRIRGSNIHEPVTAMVAVENTHN 670
+ DG + + + + +N H PV+ +A + T +
Sbjct: 257 STMKAKDG---VHAVALQHKTANGHPPVSYAIAAKETED 292
>At4g10060.1 68417.m01645 expressed protein contains Pfam domain
PF04685: Protein of unknown function, DUF608
Length = 912
Score = 30.7 bits (66), Expect = 0.99
Identities = 23/99 (23%), Positives = 43/99 (43%), Gaps = 3/99 (3%)
Frame = +2
Query: 383 LLGKQAALFVPSGTMSNLIAIMVH---CNKRGSEAFVGDLSHIYKYEVGGASHLAGVMLS 553
++ +Q + F+P + + + V + G E L ++ VGGAS L G +
Sbjct: 192 IVSRQVSPFIPHNYKESSLPVSVFEFTMSNLGKEEATVTLLFTWENSVGGASGLTGEHFN 251
Query: 554 TVANKPDGTFDLQELEDRIRGSNIHEPVTAMVAVENTHN 670
+ + DG + + + N H PVT +A + T +
Sbjct: 252 STIMERDG---VHAIVLHHKTGNGHPPVTYAIAAQETED 287
>At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloroplast,
putative / O-succinylhomoserine (Thiol)-lyase, putative
strong similarity to SP|P55217 Cystathionine
gamma-synthase, chloroplast precursor (EC 4.2.99.9)
(CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis
thaliana}; contains Pfam profile PF01053: Cys/Met
metabolism PLP-dependent enzyme
Length = 412
Score = 28.7 bits (61), Expect = 4.0
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = +2
Query: 335 YGEDPTVNALENKIAALLGKQAALFVPSGTMSNLIAIM 448
YG +PT ALE KI+ L G ++ L + SG ++ + ++
Sbjct: 79 YG-NPTTMALEEKISVLEGAESTLVMASGMYASNVMLL 115
>At3g13222.1 68416.m01655 expressed protein
Length = 567
Score = 28.3 bits (60), Expect = 5.3
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -2
Query: 582 KVPSGLLATVESITPAKCDAPPTSY 508
KV + +L ++ AKCDAPP SY
Sbjct: 345 KVKNEVLPITDTHQAAKCDAPPISY 369
>At1g64660.1 68414.m07330 Cys/Met metabolism
pyridoxal-phosphate-dependent enzyme family protein
similar to SP|P13254 Methionine gamma-lyase (EC
4.4.1.11) (L-methioninase) {Pseudomonas putida};
contains Pfam profile PF01053: Cys/Met metabolism
PLP-dependent enzyme
Length = 441
Score = 28.3 bits (60), Expect = 5.3
Identities = 18/43 (41%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
Frame = +2
Query: 344 DPTVNALENKIAALLGKQAALFVPSGTMSNLIAIMVH-CNKRG 469
+PTV L ++AAL G QAA SG MS + ++M+ C+ G
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSG-MSAISSVMLQLCSSGG 141
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,154,559
Number of Sequences: 28952
Number of extensions: 257005
Number of successful extensions: 559
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 546
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 555
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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