BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10k02 (734 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosacchar... 151 8e-38 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 31 0.23 SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 27 3.7 SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac... 26 6.4 SPAC16E8.11c |tfb1||transcription factor TFIIH complex subunit T... 26 6.4 SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 25 8.5 >SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 151 bits (367), Expect = 8e-38 Identities = 78/187 (41%), Positives = 114/187 (60%), Gaps = 1/187 (0%) Frame = +2 Query: 86 MKARRDGKEEDSNVFQNLDKTTLLQEARYFNSTPVHPRKCIHILTKILYLLNQGEELTTQ 265 M + + D ++F N+++ T+ Q+AR FNS+ + PRK +L+KI YL+ GE + Sbjct: 1 MSYSKKDDDGDESIFANVNQVTVTQDARAFNSSSISPRKSRRLLSKIAYLIYTGEHFQEK 60 Query: 266 EATDIFFATTKLFQSKDVVLRRLVYLCIKELSPMAQDVIIVTSSLTKD-MTGKDDEYRPA 442 +AT++FF TKLFQ KD LR+ VY+ IKELS +A+DVI++TSS+ KD TG++ YRP Sbjct: 61 QATELFFGITKLFQHKDPSLRQFVYIIIKELSVVAEDVIMITSSIMKDTATGRETIYRPN 120 Query: 443 AIRALCSITDSTMLQAIERYMKQAIVDKNPXXXXXXXXXXXXXXXXXPDLVRRWINEAQE 622 AIR+L + D+ + AIER + IVD D+V RW NE Q+ Sbjct: 121 AIRSLIRVIDANTVPAIERILTTGIVDPISAVASAALVSAYHLYPVAKDIVSRWNNEVQD 180 Query: 623 AMTSDHV 643 A+TS +V Sbjct: 181 AVTSHNV 187 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 30.7 bits (66), Expect = 0.23 Identities = 33/146 (22%), Positives = 61/146 (41%) Frame = -3 Query: 684 LAPATTAKAW*ETIT*SEVMASCASFIQRRTKSGTVADRCKAETSAADPTAGFLSTIACF 505 L ATT+ + + S + S ++ T S ++A T++A PT+ L++ + Sbjct: 56 LKRATTSYNYNTSSASSSSLTSSSAASSSLTSSSSLASSSTNSTTSASPTSSSLTSSSAT 115 Query: 504 MYLSIACSIVLSVMLHKARMAAGRYSSSLPVMSFVSEEVTMITSCAIGLNSLMHR*TKRR 325 + S S + + ++ SSS+ S S T + + NS T Sbjct: 116 SSSLASSSTTSSSLASSSITSSSLASSSITSSSLASSSTTSSSLASSSTNSTTSA-TPTS 174 Query: 324 NTTSFD*NSFVVAKNISVASCVVNSS 247 + TS +S A N + +S + +SS Sbjct: 175 SATSSSLSS-TAASNSATSSSLASSS 199 >SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 26.6 bits (56), Expect = 3.7 Identities = 24/84 (28%), Positives = 36/84 (42%), Gaps = 1/84 (1%) Frame = -3 Query: 729 LVFVMSFTVDNLSLRLAPATTAKAW*ETIT*SEVMASCASFIQRRTKS-GTVADRCKAET 553 L + S + DNL+ + P T A + ++ A+ +S + T S TV + Sbjct: 169 LASLSSASTDNLAASVTPTTVAASVASSVDTDSASATISSVVDSATSSVSTVIP--SSII 226 Query: 552 SAADPTAGFLSTIACFMYLSIACS 481 SAA P + ST A Y S S Sbjct: 227 SAAPPDSASESTPASTSYASSTTS 250 >SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 741 Score = 25.8 bits (54), Expect = 6.4 Identities = 12/27 (44%), Positives = 14/27 (51%) Frame = +2 Query: 173 FNSTPVHPRKCIHILTKILYLLNQGEE 253 F PV I KIL+LLN+G E Sbjct: 281 FPQPPVESHSSFDIKQKILHLLNEGNE 307 >SPAC16E8.11c |tfb1||transcription factor TFIIH complex subunit Tfb1|Schizosaccharomyces pombe|chr 1|||Manual Length = 477 Score = 25.8 bits (54), Expect = 6.4 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = -3 Query: 144 LSRFWKTLLSSSFPSRRAFIML 79 L +FW LL+++FP ++A L Sbjct: 401 LHQFWNALLNTTFPDKKAMAPL 422 >SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1689 Score = 25.4 bits (53), Expect = 8.5 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +2 Query: 110 EEDSNVFQNLDKTTLLQEARYFNSTPV 190 EED N +N K+ + A+Y ST V Sbjct: 302 EEDPNPLKNKSKSPIAHGAKYMTSTEV 328 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,074,993 Number of Sequences: 5004 Number of extensions: 63775 Number of successful extensions: 188 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 182 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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