Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte10j13
(669 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 148 3e-36
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 146 9e-36
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 142 2e-34
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 141 3e-34
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 141 4e-34
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 137 5e-33
At5g31752.1 68418.m03682 hypothetical protein 28 6.5
At3g45780.1 68416.m04953 protein kinase / nonphototropic hypocot... 28 6.5
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 148 bits (359), Expect = 3e-36
Identities = 77/188 (40%), Positives = 103/188 (54%), Gaps = 4/188 (2%)
Frame = +2
Query: 116 QNKVQDNPKILSGPVLQ-NSPNAFLSKTLLDRYRDLPLPEDKVLATYVWIDGSGEHVRCK 292
+ K Q N K+ VL S N+ +++ D D+++A Y+WI GSG +R K
Sbjct: 35 KQKKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSK 94
Query: 293 DRTLNYIPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAIYRDPFRRGNHVLVMCDTYD 472
RT+ E P +LP WNY L PQAI+RDPFR GN++LV+CDT+
Sbjct: 95 SRTIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFRDPFRGGNNILVICDTWT 154
Query: 473 YSMQPTKTNHRIKCQEAYD--RCRDHEPWFGIEQEYVLLDSDLR-PFGWPTTGSPPAQGP 643
+ +P TN R K E + + PWFGIEQEY LL +++ P GWP P QGP
Sbjct: 155 PAGEPIPTNKRAKAAEIFSNKKVSGEVPWFGIEQEYTLLQQNVKWPLGWPVGAFPGPQGP 214
Query: 644 YYCGVGAN 667
YYCGVGA+
Sbjct: 215 YYCGVGAD 222
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 146 bits (355), Expect = 9e-36
Identities = 70/147 (47%), Positives = 87/147 (59%), Gaps = 3/147 (2%)
Frame = +2
Query: 236 KVLATYVWIDGSGEHVRCKDRTLNYIPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAI 415
K++A Y+WI GSG +R K RTL P LP WNY L PQAI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVILYPQAI 77
Query: 416 YRDPFRRGNHVLVMCDTYDYSMQPTKTNHRIKCQEAYD--RCRDHEPWFGIEQEYVLLDS 589
++DPFR+GN++LVMCD Y + P TN+R K + +D + EPWFGIEQEY LL
Sbjct: 78 FKDPFRKGNNILVMCDAYRPAGDPIPTNNRHKAVKIFDHPNVKAEEPWFGIEQEYTLLKK 137
Query: 590 DLR-PFGWPTTGSPPAQGPYYCGVGAN 667
D++ P GWP G P QGPYYC VGA+
Sbjct: 138 DVKWPLGWPLGGFPGPQGPYYCAVGAD 164
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 142 bits (344), Expect = 2e-34
Identities = 67/148 (45%), Positives = 85/148 (57%), Gaps = 3/148 (2%)
Frame = +2
Query: 233 DKVLATYVWIDGSGEHVRCKDRTLNYIPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQA 412
DK++A Y+W+ GSG +R K RTL P LP WNY L PQA
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVILYPQA 76
Query: 413 IYRDPFRRGNHVLVMCDTYDYSMQPTKTNHRIKCQEAYDR--CRDHEPWFGIEQEYVLLD 586
I++DPFRRGN++LVMCD Y + +P TN R + + PW+GIEQEY LL
Sbjct: 77 IFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVFSNPDVAAEVPWYGIEQEYTLLQ 136
Query: 587 SDLR-PFGWPTTGSPPAQGPYYCGVGAN 667
D++ P GWP G P QGPYYCG+GA+
Sbjct: 137 KDVKWPVGWPIGGYPGPQGPYYCGIGAD 164
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 141 bits (342), Expect = 3e-34
Identities = 67/147 (45%), Positives = 83/147 (56%), Gaps = 3/147 (2%)
Frame = +2
Query: 236 KVLATYVWIDGSGEHVRCKDRTLNYIPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAI 415
K++A Y+WI GSG +R K RTL P LP WNY L PQAI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAI 77
Query: 416 YRDPFRRGNHVLVMCDTYDYSMQPTKTNHRIKCQEAYDR--CRDHEPWFGIEQEYVLLDS 589
++DPFR+GN++LVMCD Y + P TN R + + EPW+GIEQEY L+
Sbjct: 78 FKDPFRKGNNILVMCDAYTPAGDPIPTNKRHNAAKIFSHPDVAKEEPWYGIEQEYTLMQK 137
Query: 590 DLR-PFGWPTTGSPPAQGPYYCGVGAN 667
D+ P GWP G P QGPYYCGVGA+
Sbjct: 138 DVNWPIGWPVGGYPGPQGPYYCGVGAD 164
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 141 bits (341), Expect = 4e-34
Identities = 69/154 (44%), Positives = 88/154 (57%), Gaps = 3/154 (1%)
Frame = +2
Query: 215 DLPLPEDKVLATYVWIDGSGEHVRCKDRTLNYIPETPKDLPIWNYXXXXXXXXXXXXXXX 394
DL D+++A Y+WI GSG +R K RTL P LP WNY
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEV 70
Query: 395 HLKPQAIYRDPFRRGNHVLVMCDTYDYSMQPTKTNHRIKCQEAYD--RCRDHEPWFGIEQ 568
+ PQAI++DPFRRGN++LVMCD Y + +P TN R + ++ E W+GIEQ
Sbjct: 71 IIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFEDPSVVAEETWYGIEQ 130
Query: 569 EYVLLDSDLR-PFGWPTTGSPPAQGPYYCGVGAN 667
EY LL D++ P GWP G P QGPYYCGVGA+
Sbjct: 131 EYTLLQKDIKWPVGWPVGGFPGPQGPYYCGVGAD 164
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 137 bits (332), Expect = 5e-33
Identities = 67/154 (43%), Positives = 85/154 (55%), Gaps = 3/154 (1%)
Frame = +2
Query: 215 DLPLPEDKVLATYVWIDGSGEHVRCKDRTLNYIPETPKDLPIWNYXXXXXXXXXXXXXXX 394
D+ +K++A Y+W+ GSG +R K RTL P LP WNY
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEV 70
Query: 395 HLKPQAIYRDPFRRGNHVLVMCDTYDYSMQPTKTNHRIKCQEAYDR--CRDHEPWFGIEQ 568
L PQAI++DPFRRGN++LVMCD Y + +P TN R E + PW+GIEQ
Sbjct: 71 ILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAEIFANPDVIAEVPWYGIEQ 130
Query: 569 EYVLLDSDLR-PFGWPTTGSPPAQGPYYCGVGAN 667
EY LL D+ P GWP G P QGPYYC +GA+
Sbjct: 131 EYTLLQKDVNWPLGWPIGGFPGPQGPYYCSIGAD 164
>At5g31752.1 68418.m03682 hypothetical protein
Length = 416
Score = 27.9 bits (59), Expect = 6.5
Identities = 14/37 (37%), Positives = 17/37 (45%)
Frame = +2
Query: 233 DKVLATYVWIDGSGEHVRCKDRTLNYIPETPKDLPIW 343
DK VWID + K + Y PE K LPI+
Sbjct: 193 DKAADKRVWIDDESSNKMKKKKKKTYGPEEEKALPIF 229
>At3g45780.1 68416.m04953 protein kinase / nonphototropic hypocotyl
protein 1 (NPH1) / phototropin identical to SP|O48963
Nonphototropic hypocotyl protein 1 (EC 2.7.1.37)
(Phototropin) {Arabidopsis thaliana}, cDNA
nonphototropic hypocotyl 1 (NPH1) GI:2832240; contains
Pfam profiles PF00069:Protein kinase domain and
PF00785:PAC motif
Length = 996
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -3
Query: 580 EHVLLLYAEPRLVVSAAVVRFLAFNTVVCLCWLH 479
E +LL +PR V+ VRF A VV L +LH
Sbjct: 747 ELFMLLDRQPRKVLKEDAVRFYAAQVVVALEYLH 780
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,064,186
Number of Sequences: 28952
Number of extensions: 325738
Number of successful extensions: 889
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 854
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 871
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -