Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte10h07
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 178 2e-45
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 178 3e-45
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 175 2e-44
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 174 5e-44
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 109 1e-24
At1g54860.1 68414.m06263 expressed protein 28 5.2
At3g62570.1 68416.m07029 DNAJ heat shock N-terminal domain-conta... 27 9.0
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 27 9.0
At1g62790.2 68414.m07088 protease inhibitor/seed storage/lipid t... 27 9.0
At1g62790.1 68414.m07087 protease inhibitor/seed storage/lipid t... 27 9.0
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 178 bits (434), Expect = 2e-45
Identities = 83/172 (48%), Positives = 118/172 (68%)
Frame = +1
Query: 184 AFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIG 363
A G+ +G++IWRI+NF+P+ V Q+ GKF+ GDSYI+L+TT+ +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 364 RESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFN 543
++S+QDE+GA A++TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SGFN
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFN 130
Query: 544 HVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
HV Q R++ KGK VRV++V +++N DVF+LD ++ I+ F G
Sbjct: 131 HVKPEE-HQTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSG 181
Score = 52.4 bits (120), Expect = 3e-07
Identities = 46/216 (21%), Positives = 91/216 (42%), Gaps = 10/216 (4%)
Frame = +1
Query: 82 GSVWARTATKPQEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQIWRIQNFEPIPVAQ 261
G V A + + G + S +++D+ K + + T +Q+WRI E I +
Sbjct: 358 GKVAALLQRQGVNVQG-LVKTSSSSSKDEPKPY-----IDGTGNLQVWRINCEEKILLEA 411
Query: 262 KDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAA 441
+ KFY GD Y IL+ + + + W G++S +++ +A L + +
Sbjct: 412 AEQSKFYSGDCY-ILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVP 470
Query: 442 VQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVV--------TNAGAQKRMFQVKGK 597
Q R G E F Q+ + + +GG F + T +F+V+G
Sbjct: 471 AQARINEGKEPIQFFVIMQSFITF-KGGVSDAFKKYIAENDIPDTTYEAEGVALFRVQGS 529
Query: 598 --RDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
+++ Q++ A +N ++L D+ ++ + G
Sbjct: 530 GPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCG 565
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 178 bits (433), Expect = 3e-45
Identities = 85/173 (49%), Positives = 115/173 (66%)
Frame = +1
Query: 181 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 360
PAF G+ G +IWRI+NFE +PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 361 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 540
G++++QDE+G AA+ TV LD GG AVQHRE GHES FLSYF+ + LEGG SGF
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 541 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
V + R++ KGKR +R++QV +S+N DVF+LD + IY F G
Sbjct: 128 K-TVEEEVFETRLYTCKGKRAIRLKQVPFARSSLNHDDVFILDTEEKIYQFNG 179
Score = 64.5 bits (150), Expect = 6e-11
Identities = 42/172 (24%), Positives = 78/172 (45%), Gaps = 10/172 (5%)
Frame = +1
Query: 214 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGA 393
+++W + P+ ++DIGK Y GD Y++L T + + W G++S ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDT 451
Query: 394 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGAQK 573
A L + + G VQ R G E F++ FQ P+ L+GG SG+ + + +
Sbjct: 452 AIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQ-PMVVLKGGLSSGYKSSMGESESTD 510
Query: 574 RMF--------QVKGK--RDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
+ QV G + + QV+ S+N + F+L ++++ G
Sbjct: 511 ETYTPESIALVQVSGTGVHNNKAVQVETVATSLNSYECFLLQSGTSMFLWHG 562
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 175 bits (427), Expect = 2e-44
Identities = 85/173 (49%), Positives = 114/173 (65%)
Frame = +1
Query: 181 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 360
PAF VG+ G +IWRI+NFEP+PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 361 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 540
G++++QDE+G AA+ TV LD GG AVQ+RE GHES FLSYF+ + LEGG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Query: 541 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
+ R++ KGKR V ++QV +S+N DVF+LD IY F G
Sbjct: 130 KK-PEEEEFETRLYTCKGKRAVHLKQVPFARSSLNHDDVFILDTKEKIYQFNG 181
Score = 67.3 bits (157), Expect = 9e-12
Identities = 44/172 (25%), Positives = 78/172 (45%), Gaps = 10/172 (5%)
Frame = +1
Query: 214 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGA 393
+++W I +++ +GK Y GD Y++L T + + W G+ S Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 394 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGAQK 573
A L + + G VQ R G E F++ FQ + L+GG SG+ + +T G+
Sbjct: 454 AVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQ-HMVVLKGGLSSGYKNSMTEKGSSG 512
Query: 574 R--------MFQVKGK--RDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
+ QV G + + QV+ S+N D F+L ++++VG
Sbjct: 513 ETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVG 564
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 174 bits (423), Expect = 5e-44
Identities = 82/173 (47%), Positives = 114/173 (65%)
Frame = +1
Query: 181 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 360
PAF G+ AG++IWRI+NF P P+ + IGKF+ GDSYI+L+TT+ L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 361 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 540
G++++QDE+G AA+ TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGF 129
Query: 541 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
HVV R+F +GK V V++V +S+N D+++LD + I+ F G
Sbjct: 130 KHVVAEEHI-TRLFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNG 181
Score = 58.0 bits (134), Expect = 6e-09
Identities = 41/175 (23%), Positives = 72/175 (41%), Gaps = 10/175 (5%)
Frame = +1
Query: 205 TAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDE 384
T +Q+WR+ + D KFY GD Y + + + I W G++S ++E
Sbjct: 391 TGNLQVWRVNGQAKTLLQAADHSKFYSGDCY-VFQYSYPGEEKEEVLIGTWFGKQSVEEE 449
Query: 385 SGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNA- 561
G+A + + + Q R G E F Q+ + + +GG SG+ +
Sbjct: 450 RGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSGYKKYIAEKE 508
Query: 562 ---------GAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
G Q G +++ QVDP AS+N ++L D+ ++ + G
Sbjct: 509 VDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAG 563
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 109 bits (263), Expect = 1e-24
Identities = 57/175 (32%), Positives = 96/175 (54%)
Frame = +1
Query: 175 VHPAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHY 354
+ AF VG +G++IW + N + I + + GKF+ G++Y++LRT + +DIHY
Sbjct: 8 IDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIHY 67
Query: 355 WIGRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPS 534
W+G ++ + +S A+ + LD G VQ+RE G E+ FLSYF+ + +EG
Sbjct: 68 WLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKYSP 127
Query: 535 GFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
+ Q + + KG VRV++V +S+N DVF+LD + +++F G
Sbjct: 128 KTG--IAGETYQVTLLRCKGDHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAG 180
Score = 34.3 bits (75), Expect = 0.079
Identities = 36/174 (20%), Positives = 73/174 (41%), Gaps = 12/174 (6%)
Frame = +1
Query: 214 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILR--TTSDNRNNLSWDIHYWIGRESTQDES 387
+++WR+ + ++ D K + GD Y++ T + +L ++ WIG ES Q +
Sbjct: 391 LKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL---LYVWIGCESIQQDR 447
Query: 388 GAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGA 567
A + G +V G+E + F FQ+ L +GG + ++
Sbjct: 448 ADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQS-LVVFKGGLSRRYKVLLAEKEK 506
Query: 568 --------QKRMFQVKG--KRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 699
+ +F+V G R+++ QV+ S+N ++L + ++G
Sbjct: 507 IGEEYNENKASLFRVVGTSPRNMQAIQVNLVATSLNSSYSYILQYGASAFTWIG 560
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -2
Query: 230 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 129
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
>At3g62570.1 68416.m07029 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profiles
PF00226: DnaJ domain, PF00515: TPR Domain
Length = 552
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 2/47 (4%)
Frame = +1
Query: 43 MKIIFSCLLF--VLLGSVWARTATKPQEISGPITSLSDKNARDKAKV 177
+KI+++ +L L G W R K +EI G + L+ K+ + KAK+
Sbjct: 340 LKILYNTILRDRKLPGPPWKRHNVKYREIPGKLCELTTKSKKLKAKM 386
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.5 bits (58), Expect = 9.0
Identities = 30/120 (25%), Positives = 48/120 (40%)
Frame = +1
Query: 97 RTATKPQEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQIWRIQNFEPIPVAQKDIGK 276
R P+E + SLS A+ KAK V R+ + W I I A K +
Sbjct: 134 RKEASPEECDQAVESLSSVKAKAKAKRLQESKKVARSIVQRAWAI--VLKIGPAIKAVAS 191
Query: 277 FYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQHRE 456
+ D + T +S HYW+G + ++ ++ L + L GG ++ RE
Sbjct: 192 MNRAD--WAKKLTHWKHEFVSTLKHYWLGTKLLWADTRISSRLLLKL---AGGKSLSRRE 246
>At1g62790.2 68414.m07088 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 149
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -3
Query: 364 DRSSSGCPMTDCCDCPRSSVK*CMNLLCRIFLCP 263
D +++ P+ +CCD + +V+ + LC I+ P
Sbjct: 39 DLNTTTTPVKECCDSIKEAVEKELTCLCTIYTSP 72
>At1g62790.1 68414.m07087 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 150
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -3
Query: 364 DRSSSGCPMTDCCDCPRSSVK*CMNLLCRIFLCP 263
D +++ P+ +CCD + +V+ + LC I+ P
Sbjct: 39 DLNTTTTPVKECCDSIKEAVEKELTCLCTIYTSP 72
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,655,365
Number of Sequences: 28952
Number of extensions: 349066
Number of successful extensions: 970
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 925
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 963
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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