BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10c17 (748 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ... 93 1e-19 At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 93 2e-19 At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ... 76 2e-14 At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ... 61 7e-10 At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ... 57 1e-08 At5g48700.1 68418.m06027 ubiquitin-related contains similarity t... 52 5e-07 At5g55855.1 68418.m06962 hypothetical protein 45 6e-05 At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-... 43 2e-04 >At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 from [Arabidopsis thaliana]; identical to cDNA small ubiquitin-like modifier 2 (SUMO) GI:22652843; contains Pfam profile PF00240: Ubiquitin family Length = 103 Score = 93.5 bits (222), Expect = 1e-19 Identities = 43/85 (50%), Positives = 54/85 (63%) Frame = +1 Query: 118 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPIN 297 D+K + HINLKV GQD V F+IK+ T L+KLMNAYCDR + + F FDG+ + Sbjct: 8 DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSIAFLFDGRRLR 67 Query: 298 ENDTPTSLEMEEGDTIEVYQQQTGG 372 TP LEME+GD I+ QTGG Sbjct: 68 AEQTPDELEMEDGDEIDAMLHQTGG 92 >At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis thaliana]; identical to cDNA SMT3 protein GI:1707371 Length = 100 Score = 92.7 bits (220), Expect = 2e-19 Identities = 44/86 (51%), Positives = 56/86 (65%), Gaps = 1/86 (1%) Frame = +1 Query: 118 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPI 294 D+K G+ HINLKV GQD V F+IK+ T L+KLMNAYCDR + M + F FDG+ + Sbjct: 8 DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSIAFLFDGRRL 67 Query: 295 NENDTPTSLEMEEGDTIEVYQQQTGG 372 TP L+ME+GD I+ QTGG Sbjct: 68 RAEQTPDELDMEDGDEIDAMLHQTGG 93 >At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe}; identical to cDNA small ubiquitin-like modifier 3 (SUMO) GI:22652845 Length = 111 Score = 76.2 bits (179), Expect = 2e-14 Identities = 36/81 (44%), Positives = 48/81 (59%) Frame = +1 Query: 133 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPINENDTP 312 + H+ LKV QD V FK KK PL+KLM YCDR GL + F F+G I +TP Sbjct: 14 QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKLDAFAFIFNGARIGGLETP 73 Query: 313 TSLEMEEGDTIEVYQQQTGGV 375 L+ME+GD I+ + +GG+ Sbjct: 74 DELDMEDGDVIDACRAMSGGL 94 >At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 [Arabidopsis thaliana]; contains INTERPRO:IPR000626 ubiquitin domain; contains Pfam profile PF00240: Ubiquitin family; contains Pfam profile PF00240: Ubiquitin family; identical to cDNA small ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847 Length = 108 Score = 61.3 bits (142), Expect = 7e-10 Identities = 32/91 (35%), Positives = 46/91 (50%) Frame = +1 Query: 100 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRF 279 ++ K + + + LKV Q A +KI H L+KLM+AYC + L VRF + Sbjct: 13 VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSVRFVY 72 Query: 280 DGQPINENDTPTSLEMEEGDTIEVYQQQTGG 372 +G+ I TP L MEE D I + + GG Sbjct: 73 NGREIKARQTPAQLHMEEEDEICMVMELGGG 103 >At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe} Length = 114 Score = 57.2 bits (132), Expect = 1e-08 Identities = 32/93 (34%), Positives = 47/93 (50%), Gaps = 4/93 (4%) Frame = +1 Query: 106 LKMADEKK---GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVRF 273 +KM +K+ + H+ LKV GQD + F ++++ L K+M Y G+ RF Sbjct: 12 VKMEGQKRKVVSDPTHVTLKVKGQDEEDFRVFWVRRNAKLLKMMELYTKMRGIEWNTFRF 71 Query: 274 RFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 372 FDG I E TP LE ++GD I+ Q G Sbjct: 72 LFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104 >At5g48700.1 68418.m06027 ubiquitin-related contains similarity to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe} Length = 117 Score = 51.6 bits (118), Expect = 5e-07 Identities = 32/95 (33%), Positives = 47/95 (49%), Gaps = 6/95 (6%) Frame = +1 Query: 106 LKMADEKK----GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVR 270 +KM EK+ E+ H+ L V GQD V+ F++++ L KLM Y G+ R Sbjct: 14 VKMEGEKRKDVESESTHVTLNVKGQDEEGVKVFRVRRKARLLKLMEYYAKMRGIEWNTFR 73 Query: 271 FRFD-GQPINENDTPTSLEMEEGDTIEVYQQQTGG 372 F D G I E T +E+++GD I+ Q G Sbjct: 74 FLSDDGSRIREYHTADDMELKDGDQIDALLPQESG 108 >At5g55855.1 68418.m06962 hypothetical protein Length = 59 Score = 44.8 bits (101), Expect = 6e-05 Identities = 22/56 (39%), Positives = 29/56 (51%), Gaps = 3/56 (5%) Frame = +1 Query: 220 LMNAYCDRAGLSMQVVRFRFDGQPINENDTPT---SLEMEEGDTIEVYQQQTGGVS 378 +M AY D+ G M RF DG I N TP L++E+GD I+ + Q G S Sbjct: 1 MMQAYSDKVGQQMSAFRFHCDGIRIKPNQTPNEELQLDLEDGDEIDAFVDQIAGFS 56 >At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission yeast] Length = 215 Score = 42.7 bits (96), Expect = 2e-04 Identities = 20/50 (40%), Positives = 32/50 (64%) Frame = +1 Query: 217 KLMNAYCDRAGLSMQVVRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQT 366 +++ Y D+A L Q + F FDG I+ + TP+ L ME+ D IEV+ ++T Sbjct: 166 RVIKLYTDKAKLDPQNLVFIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,847,131 Number of Sequences: 28952 Number of extensions: 284953 Number of successful extensions: 592 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 579 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 592 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1653386488 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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