BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10c15 (743 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 100 4e-22 SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 38 0.002 SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo... 29 0.53 SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.92 SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 2.8 SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 26 4.9 SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 26 6.5 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 6.5 SPAC4G9.17c |mrps5||mitochondrial ribosomal protein subunit S5|S... 25 8.6 SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 8.6 SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 8.6 >SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|||Manual Length = 137 Score = 99.5 bits (237), Expect = 4e-22 Identities = 55/140 (39%), Positives = 81/140 (57%) Frame = +2 Query: 95 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 274 SGV VS C ++E+K K RYVVF + D K V + +++ FL DL + Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60 Query: 275 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 454 +CRY ++DFE+ + +G + K+ +SW PD A +K KM+YSSS D L+++ G+ Sbjct: 61 DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114 Query: 455 KYIQATDLSEASQEAVEEKL 514 IQATD SE + E V EK+ Sbjct: 115 TDIQATDFSEVAYETVLEKV 134 >SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 37.9 bits (84), Expect = 0.002 Identities = 28/86 (32%), Positives = 44/86 (51%) Frame = +2 Query: 161 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 340 R + I +E DV+T+ E++ E D +K EC G + + + S Sbjct: 26 RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76 Query: 341 KKQKLFLMSWCPDTAKVKKKMLYSSS 418 KK L L+S+ P+ A V++KMLY+SS Sbjct: 77 KKNLLQLISYVPENANVRRKMLYASS 102 Score = 28.7 bits (61), Expect = 0.92 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%) Frame = +2 Query: 350 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 502 K+ + CP A VK +M+YSSS D++K L + + I++ D ++ +++ + Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299 >SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 29.5 bits (63), Expect = 0.53 Identities = 17/63 (26%), Positives = 30/63 (47%) Frame = +2 Query: 326 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 505 T++ LF++ W P M+Y+S+ + + V K +A D + + EAV+ Sbjct: 79 TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137 Query: 506 EKL 514 E L Sbjct: 138 EFL 140 >SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 314 Score = 28.7 bits (61), Expect = 0.92 Identities = 37/174 (21%), Positives = 67/174 (38%), Gaps = 3/174 (1%) Frame = +2 Query: 68 LRE*HQKMASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGE-RNAEYEQF 244 LR ++ G D+ ++ +E ++KK + F RD++++ ++ R Sbjct: 41 LRSEGSELIFGGRSFDSLLSSIKETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTPRNRTQ 100 Query: 245 LEDLQ--KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSS 418 + D + + T +F+ Q T A+K + P T K + ++ Sbjct: 101 IIDARSTRKDTENESLRKNNFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAE 160 Query: 419 FDALKKSLVGVQKYIQATDLSEASQEAVEEKLRATDRQ*TAFTHELATKPNPLS 580 A KK + G +KY + S + E K R DR + FT LS Sbjct: 161 RPASKKPVFGSKKYFDVINDSNVENKE-ETKDRNLDRALSKFTQSREKNEQNLS 213 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 27.1 bits (57), Expect = 2.8 Identities = 10/26 (38%), Positives = 19/26 (73%) Frame = +2 Query: 416 SFDALKKSLVGVQKYIQATDLSEASQ 493 S +AL++ L G + Y+Q+TD++ S+ Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213 >SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr 2|||Manual Length = 319 Score = 26.2 bits (55), Expect = 4.9 Identities = 26/136 (19%), Positives = 60/136 (44%), Gaps = 1/136 (0%) Frame = +2 Query: 122 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 301 K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166 Query: 302 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLS 481 + + G S+K ++ A V K ++ +K++ K ++ + Sbjct: 167 Q--KKVHGPPALSEKPGKCILK-DKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEFE 223 Query: 482 EASQ-EAVEEKLRATD 526 ++ E EEK + ++ Sbjct: 224 SINEGETEEEKAKTSN 239 >SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 447 Score = 25.8 bits (54), Expect = 6.5 Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 3/60 (5%) Frame = +1 Query: 478 LGSVSGGRRREAPRHRSPINSIYTRARDE---TEPALRHSCPDDTRPRHH*PLSIITKEN 648 LG G ++ E+ R + P S + EP +RHS RP+ H S +T+++ Sbjct: 20 LGEEDGRQKGESSRKKRPPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.8 bits (54), Expect = 6.5 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%) Frame = -3 Query: 369 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 253 HD+ +++ S +H C ++S P+ LH+P+P C+ Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150 >SPAC4G9.17c |mrps5||mitochondrial ribosomal protein subunit S5|Schizosaccharomyces pombe|chr 1|||Manual Length = 387 Score = 25.4 bits (53), Expect = 8.6 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = +1 Query: 28 CRCRGVGEVSGVIFT*ITSKNGV 96 CRC G+ ++SG I + SKNG+ Sbjct: 330 CRCAGIKDISGEI---LGSKNGM 349 >SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor complex subunit Rrn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 25.4 bits (53), Expect = 8.6 Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 4/92 (4%) Frame = -3 Query: 585 VSESGFGFVASSCVNAVYWRSVARSFSSTAS*DASERSVAWMYF*TPTR---DFFRASNE 415 + E +G ++ SCV+ YW + + A +R + + + P++ R N Sbjct: 56 LEEEQWGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINA 115 Query: 414 LEYNIFFLTLAVSGHHDIR-KSFCFLLASDVP 322 LE + +L H D +S C L D+P Sbjct: 116 LENELRWL----RNHVDASVRSQCLLKYEDIP 143 >SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 357 Score = 25.4 bits (53), Expect = 8.6 Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%) Frame = -3 Query: 321 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 190 W++ Y+ S R Y VPP+CR +R+ + CF Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,714,500 Number of Sequences: 5004 Number of extensions: 51723 Number of successful extensions: 173 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 170 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 353266144 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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