Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte10b24
         (715 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   307   1e-84
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   301   8e-83
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   165   7e-42
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   135   7e-33
SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces po...    30   0.38 
SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual        26   6.1  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  307 bits (753), Expect = 1e-84
 Identities = 134/205 (65%), Positives = 165/205 (80%)
 Frame = +2

Query: 101 MRECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGK 280
           MRE ISIH GQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 281 HVPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPR++++DLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 461 RVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQISTA 640
           ++R++AD C+GLQGFL+              LL+ERL+++Y KKSKL+F++YPAPQ+ST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 641 VVEPYNSILTTHTTLEHSDAAFMVD 715
           VVEPYNS+LTTH TL+ +D  FMVD
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVD 205


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  301 bits (738), Expect = 8e-83
 Identities = 132/209 (63%), Positives = 166/209 (79%), Gaps = 4/209 (1%)
 Frame = +2

Query: 101 MRECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGD----DSFNTFFSET 268
           MRE IS+H GQAGVQIGNACWELYCLEHGI PDG    +  V   +    D F TFFSET
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 269 GAGKHVPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVD 448
           G GK VPR++++DLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 449 LVLDRVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 628
            VL+R+R++AD C+GLQGFL+              LL+ERL+++YGKKS L+F++YPAPQ
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180

Query: 629 ISTAVVEPYNSILTTHTTLEHSDAAFMVD 715
           +ST+VVEPYNS+LTTH TL++SD  FMVD
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVD 209


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  165 bits (400), Expect = 7e-42
 Identities = 81/205 (39%), Positives = 115/205 (56%)
 Frame = +2

Query: 101 MRECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGK 280
           MRE + I AGQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHG--TSEAQHERLNVYFNEAAGGK 58

Query: 281 HVPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPRAV +DLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 461 RVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQISTA 640
            VR+ A+ C  LQGF +              LL+ ++  +Y  +    F++ PAP+ S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 641 VVEPYNSILTTHTTLEHSDAAFMVD 715
           VVEPYN+ L+ H  +E+SD  F +D
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCID 203


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  135 bits (326), Expect = 7e-33
 Identities = 74/207 (35%), Positives = 117/207 (56%), Gaps = 3/207 (1%)
 Frame = +2

Query: 104 RECISIHAGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGKH 283
           RE I++ AGQ G QIG+  W+  CLEHGI PDG + S  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 284 VPRAVFIDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
           +PRA+ IDLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 458 DRVRKLADQCTGLQGFLIXXXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-IS 634
           D + + AD    L+GF +               L+ERL+  Y KK    ++++P  Q +S
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 635 TAVVEPYNSILTTHTTLEHSDAAFMVD 715
             VV+PYNS+L       ++D+  ++D
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLD 206


>SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 396

 Score = 29.9 bits (64), Expect = 0.38
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = -2

Query: 579 STERRSIRSDAKPDPVPPPNEWKIRKP 499
           S +RR ++++  PD V PP  W +R P
Sbjct: 238 SDKRRFLKTNYSPDDVAPPPNWVLRYP 264


>SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 566

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 10/29 (34%), Positives = 14/29 (48%)
 Frame = -2

Query: 192 WMPCSRQYSSQHALPICTPAWPAWIDMHS 106
           W P        H +PI TPA+P+    H+
Sbjct: 286 WNPKLYPSDKAHRMPIITPAYPSMCATHN 314


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,993,920
Number of Sequences: 5004
Number of extensions: 61373
Number of successful extensions: 200
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)