BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10b20 (617 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 237 1e-63 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 133 2e-32 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 128 6e-31 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 125 6e-30 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 29 0.41 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 0.94 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.9 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 3.8 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 5.0 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 5.0 SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 26 5.0 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 5.0 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 6.6 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 6.6 SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 25 8.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 237 bits (579), Expect = 1e-63 Identities = 102/134 (76%), Positives = 118/134 (88%) Frame = +2 Query: 146 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYV 325 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 326 PRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 505 PRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 506 RKEAEGCDCLQGFQ 547 R+EAE CD LQGFQ Sbjct: 121 RREAEACDALQGFQ 134 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 133 bits (321), Expect = 2e-32 Identities = 61/135 (45%), Positives = 84/135 (62%), Gaps = 2/135 (1%) Frame = +2 Query: 146 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLER--INVYYNEASGGK 319 MREI+ I GQ G QIG WE+ EHGI G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 320 YVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 499 YVPR++ VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 500 VVRKEAEGCDCLQGF 544 +R+ A+ C LQGF Sbjct: 121 KIRRIADNCSGLQGF 135 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 128 bits (309), Expect = 6e-31 Identities = 58/139 (41%), Positives = 86/139 (61%), Gaps = 6/139 (4%) Frame = +2 Query: 146 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLER------INVYYNEA 307 MRE++ + GQ G QIG WE+ EHGI G + +S++ +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 308 SGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 487 GK+VPR++ VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 488 SVLDVVRKEAEGCDCLQGF 544 SVL+ +R+ A+ C LQGF Sbjct: 121 SVLERIRRMADNCSGLQGF 139 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 125 bits (301), Expect = 6e-30 Identities = 51/134 (38%), Positives = 91/134 (67%), Gaps = 2/134 (1%) Frame = +2 Query: 149 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYVP 328 REI+ +QAGQCGNQIG++FW+ + EHGI G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 329 RAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 502 RA+++DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 503 VRKEAEGCDCLQGF 544 + +EA+G D L+GF Sbjct: 122 IDREADGSDSLEGF 135 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 29.5 bits (63), Expect = 0.41 Identities = 29/89 (32%), Positives = 35/89 (39%), Gaps = 4/89 (4%) Frame = -1 Query: 584 YQIQYLLPENELAENPADNRSLLLLF*LHPVPNQPVQPPLCNVLSPSYSPPQ---TVRRR 414 Y YL P +A +P N L H P P PL N +SP Y P R Sbjct: 26 YTGPYLTPSGTMAYHPG-NAPLFTQAPPHTNPQGPPPFPLFNSISPVYDPATGRLLYRNV 84 Query: 413 SCPVGRFA-QTARNGRSPWFQAQGPPSQL 330 + V A NG + + GPPSQL Sbjct: 85 NTQVSHTAIPNPANGYAAVY--GGPPSQL 111 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 0.94 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -3 Query: 438 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 343 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 2.9 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 380 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 481 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 3.8 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -2 Query: 214 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 110 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 5.0 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +3 Query: 111 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 269 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 25.8 bits (54), Expect = 5.0 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +2 Query: 449 AKGHYTEGAELVDSVLDVVRKEAEGCDCLQ 538 A+GH G ELV + D +RK++E L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 25.8 bits (54), Expect = 5.0 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = -1 Query: 578 IQYLLPENELAENPADNRSLL 516 +QY+L +NEL NP + ++L Sbjct: 342 VQYILEQNELGRNPYNTTNVL 362 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 5.0 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +3 Query: 111 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 269 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 6.6 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -3 Query: 171 AWMWTISLMIALKIWNPPNRI 109 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.4 bits (53), Expect = 6.6 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = -1 Query: 488 NQPVQPPLCNVLSPSYSPPQT 426 N+P +PP+ N + PPQT Sbjct: 86 NEPAKPPIPNAATKPTFPPQT 106 >SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 982 Score = 25.0 bits (52), Expect = 8.8 Identities = 31/110 (28%), Positives = 47/110 (42%), Gaps = 5/110 (4%) Frame = +2 Query: 188 QIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYV-----PRAVMVDLE 352 QIG ++D IDA A G SD QL+ I+ + E + PR++++ E Sbjct: 742 QIGCPVPCEVADLDIIDAILARVGASDSQLKGISTFMAEMLETATILRAATPRSLIIIDE 801 Query: 353 PGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDV 502 G S G FG + + Q G +A HY E +L + + V Sbjct: 802 LGRGTSTTDG-FGLAWAITEHIVTQIGCFCLFAT-HYHEMTKLSEEITTV 849 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,614,591 Number of Sequences: 5004 Number of extensions: 55201 Number of successful extensions: 179 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 175 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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