BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10b17 (743 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.12c |||tRNA 2'-phosphotransferase Tpt1 |Schizosaccharomy... 27 2.1 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 8.6 >SPAC2C4.12c |||tRNA 2'-phosphotransferase Tpt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 27.5 bits (58), Expect = 2.1 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -3 Query: 612 EPFCMRRKTIGKSN*EMLERQPAQFGMSHNILK 514 +PFC + + E+LE + + FG S I+K Sbjct: 251 DPFCDNLQALSMHELELLEEKHSNFGYSEGIIK 283 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 25.4 bits (53), Expect = 8.6 Identities = 9/26 (34%), Positives = 17/26 (65%) Frame = -1 Query: 557 NDNRHSLECHTIF*NTDSLKETYFIK 480 +D+ HS++ HT+ SL+E F++ Sbjct: 72 DDDSHSVKIHTVISKLPSLEENVFVQ 97 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,186,459 Number of Sequences: 5004 Number of extensions: 70916 Number of successful extensions: 163 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 353266144 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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