BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10a23 (750 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 40 3e-04 SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 36 0.006 SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 28 1.2 SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 27 2.2 SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 27 2.9 SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 3.8 SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|c... 25 8.7 >SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 981 Score = 40.3 bits (90), Expect = 3e-04 Identities = 31/131 (23%), Positives = 61/131 (46%) Frame = +2 Query: 137 SGKNFFITGGTGFVGLCLIEKILRCIPDVGKVYLLMRPKKGKEISQRLEEFPKNLVFEKL 316 +G+ F +TG TG+ G +E +++ ++ V L+R + +RL +L Sbjct: 643 NGQYFLLTGATGYFGRRFLEYLVKL--NIS-VVCLVRESSDEAAKERLISLVPSLRISS- 698 Query: 317 LETNTTDIFKKLVPISGDVGEANLGLSPQDRQLLIENVNVVIHSAATLDFQENLRPTVRI 496 + ++ + V E GL + L+ENV+ + H AA + + ++ + Sbjct: 699 ---------ENIIVWAAHVEEIRFGLDDAKWEFLVENVSRIYHMAAEVHWMKSYQELRPA 749 Query: 497 NLLGTRQVMGL 529 N+LGT+ V+ L Sbjct: 750 NVLGTKTVLEL 760 >SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1419 Score = 35.9 bits (79), Expect = 0.006 Identities = 34/144 (23%), Positives = 68/144 (47%) Frame = +2 Query: 143 KNFFITGGTGFVGLCLIEKILRCIPDVGKVYLLMRPKKGKEISQRLEEFPKNLVFEKLLE 322 K F+TG G++G+ ++ ++ ++ KV L+R + +RL++ + + + Sbjct: 1005 KTVFLTGANGYLGVFILRDLMTRSSNL-KVIALVRASSEEHGLKRLKD---SCTAYGVWD 1060 Query: 323 TNTTDIFKKLVPISGDVGEANLGLSPQDRQLLIENVNVVIHSAATLDFQENLRPTVRINL 502 + +K+ ++GD+ N G+ + L E V+ VIH+ A + + N+ Sbjct: 1061 ESWA---QKISVVNGDLALENWGIEERKWNKLTEVVDYVIHNGALVHWVYPYSKLRGPNV 1117 Query: 503 LGTRQVMGLCKEIKNLKVMIHVSS 574 +GT + LC K K + VSS Sbjct: 1118 MGTITALKLCSLGKG-KSLSFVSS 1140 >SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = -2 Query: 671 HLMYRQEI*LSQPPPAQGTPSRELPLNKN*HRPMKRVSSPSN 546 H + R+ Q P TP++ +K+ RP K+ S+PS+ Sbjct: 482 HKVARKPSSTGQEPTTPSTPAKSAQSSKHARRPSKQASAPSS 523 >SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 27.5 bits (58), Expect = 2.2 Identities = 16/49 (32%), Positives = 24/49 (48%) Frame = +2 Query: 140 GKNFFITGGTGFVGLCLIEKILRCIPDVGKVYLLMRPKKGKEISQRLEE 286 GK ITGG+G +G + + +VG +Y R KK E + L + Sbjct: 9 GKTTLITGGSGGIGFSIAKAFAAAGSNVGLLY--GRNKKALEYAAELRD 55 >SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 27.1 bits (57), Expect = 2.9 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = +2 Query: 137 SGKNFFITGGTGFVGLCLIEKILR 208 SGK +TG TGF+G + E++L+ Sbjct: 2 SGKLVLVTGVTGFIGAHVAEQLLQ 25 >SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 2344 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = +2 Query: 191 IEKILRCIPDVGKVYLLMRPKKGKEISQRLEE 286 +EKIL+ PDV +YL P + I Q L++ Sbjct: 2113 VEKILKSYPDVHVIYLCCDPSR-LNIEQELQK 2143 >SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1077 Score = 25.4 bits (53), Expect = 8.7 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 53 PVNHSYISQDTPEGRNKMTEESLVRAFYSGKNFF 154 P+ S++ DT N ESL+R F G++FF Sbjct: 358 PIGGSWVEHDT----NIPNGESLIRQFLLGQHFF 387 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,810,189 Number of Sequences: 5004 Number of extensions: 53768 Number of successful extensions: 132 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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