BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte10a17
(774 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 84 9e-17
At4g12960.1 68417.m02025 gamma interferon responsive lysosomal t... 79 2e-15
At1g07080.1 68414.m00754 gamma interferon responsive lysosomal t... 77 2e-14
At4g12890.1 68417.m02017 gamma interferon responsive lysosomal t... 75 7e-14
At4g12900.1 68417.m02018 gamma interferon responsive lysosomal t... 69 5e-12
At5g01580.1 68418.m00073 gamma interferon responsive lysosomal t... 61 7e-10
At2g44580.1 68415.m05548 zinc finger (C3HC4-type RING finger) fa... 38 0.010
At3g23980.1 68416.m03012 dentin sialophosphoprotein-related cont... 30 1.5
At4g38350.1 68417.m05422 patched family protein similar to SP|O1... 30 2.0
At1g42470.1 68414.m04897 patched family protein similar to SP|O1... 29 2.6
At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family... 29 2.6
At5g43130.1 68418.m05265 transcription initiation factor IID (TF... 29 3.4
At4g03420.1 68417.m00469 expressed protein 29 4.5
At1g06490.1 68414.m00688 glycosyl transferase family 48 protein ... 29 4.5
At3g59140.1 68416.m06593 ABC transporter family protein putative... 28 6.0
At1g60180.1 68414.m06779 hypothetical protein similar to hypothe... 28 6.0
At5g38260.1 68418.m04612 serine/threonine protein kinase, putati... 28 7.9
At2g37730.1 68415.m04627 fringe-related protein similarity to pr... 28 7.9
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 84.2 bits (199), Expect = 9e-17
Identities = 63/211 (29%), Positives = 99/211 (46%), Gaps = 6/211 (2%)
Frame = +3
Query: 111 RLVCFLMLLCYVVAKKKT--EDHKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKV 281
+LV F + + + K E KV++ +YYESLCP + FI +L V+ D + V
Sbjct: 9 KLVFFACFVLFTFSHKLVTGESDKVELNLYYESLCPGCQSFIVDELVKVFDSDLDTITDV 68
Query: 282 KMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTS 461
K+VP+G + KV + IC HG +EC N ++ACV+ + + + C+ N T
Sbjct: 69 KLVPFG---YAKVSNNLTVICQHGEEECKLNALEACVINTLP-NPKSQYKFIRCVENNT- 123
Query: 462 PDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKY 641
D +CL + C +S+ L+ Y +T ++ FVP V IN
Sbjct: 124 -DNWESSCL---KGYGNEKAINDCYNSDLSKKLILGYAKQTSSLKPKHEFVPWVTIN--- 176
Query: 642 DKDVETQAFENLKAVVCRV---ATPQPSICS 725
K + T+ ++L VC+ TP P CS
Sbjct: 177 SKPLYTK-LDDLVGQVCKAYKGKTPLPIDCS 206
>At4g12960.1 68417.m02025 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 79.4 bits (187), Expect = 2e-15
Identities = 57/211 (27%), Positives = 99/211 (46%), Gaps = 6/211 (2%)
Frame = +3
Query: 111 RLVCF--LMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKV 281
+LV F L+LL + + KVK+ +YYESLCP ++FI L ++ D + +
Sbjct: 8 KLVFFGCLLLLTFTDNLVAGKSGKVKLNLYYESLCPGCQEFIVDDLGKIFDYDLYTITDL 67
Query: 282 KMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTS 461
K+ P+G + ++ + C HG +EC N ++AC L+ Q ++ + C+ + T
Sbjct: 68 KLFPFGNA---ELSDNLTVTCQHGEEECKLNALEACALRTWPDQKSQ-YSFIRCVESDT- 122
Query: 462 PDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKY 641
K ++C V + C + + L+ Y KT + P +VP V +N K
Sbjct: 123 --KGWESC---VKNSGREKAINDCYNGDLSRKLILGYATKTKNLKPPHEYVPWVTLNGK- 176
Query: 642 DKDVETQAFENLKAVVCRV---ATPQPSICS 725
D Q+ ++L A +C T P +C+
Sbjct: 177 PLDDSVQSTDDLVAQICNAYKGKTTLPKVCN 207
>At1g07080.1 68414.m00754 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 265
Score = 76.6 bits (180), Expect = 2e-14
Identities = 51/176 (28%), Positives = 90/176 (51%), Gaps = 2/176 (1%)
Frame = +3
Query: 174 KVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKMVPYGKSTHDKVDGKWSFICHH 350
KV + +YYESLCP FI LA ++ D +V + + P+G +T + D + +C H
Sbjct: 38 KVSVGLYYESLCPYCSSFIVNHLAKLFEDDLISIVDLHLSPWG-NTKLRSDNV-TAVCQH 95
Query: 351 GADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTSPDKSLDTCLTQVDKMSESDK-LK 527
GA EC+ + V+AC + D + ++ + C+ + K D T +K++ + K +
Sbjct: 96 GAFECFLDTVEACAI-DAWPKVSDHFPFIYCVEKLVTEHK-YDKWETCYEKLNLNSKPVA 153
Query: 528 RCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKYDKDVETQAFENLKAVVCR 695
C SS G+ L Y +T+A+ P +VP V++ D + +EN + +C+
Sbjct: 154 DCLSSGHGNELALHYAAETNALQPPHKYVPWVVV----DGQPLYEDYENFISYICK 205
>At4g12890.1 68417.m02017 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 232
Score = 74.5 bits (175), Expect = 7e-14
Identities = 54/191 (28%), Positives = 89/191 (46%), Gaps = 6/191 (3%)
Frame = +3
Query: 171 HKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKMVPYGKSTHDKVDGKWSFICH 347
+KVKI +YYESLCP + FI L ++ D + +K+VP+G + + + C
Sbjct: 37 NKVKINLYYESLCPYCQNFIVDDLGKIFDSDLLKITDLKLVPFGNA---HISNNLTITCQ 93
Query: 348 HGADECYGNKVQACVLKDRNLQDTE-KMEIVICLMNQTSPDKSLDTCLTQVDKMSESDKL 524
HG +EC N ++AC + R L D + + + + C+ T+ ++C V K +
Sbjct: 94 HGEEECKLNALEACGI--RTLPDPKLQYKFIRCVEKDTN---EWESC---VKKSGREKAI 145
Query: 525 KRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEK--YDKDVETQAFENLKAVVCRV 698
C + + L+ Y T ++ +VP V +N K YD + NL A VC+
Sbjct: 146 NDCYNGDLSQKLILGYAKLTSSLKPKHEYVPWVTLNGKPLYDN------YHNLVAQVCKA 199
Query: 699 --ATPQPSICS 725
P +CS
Sbjct: 200 YKGKDLPKLCS 210
>At4g12900.1 68417.m02018 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 231
Score = 68.5 bits (160), Expect = 5e-12
Identities = 45/178 (25%), Positives = 83/178 (46%), Gaps = 4/178 (2%)
Frame = +3
Query: 111 RLVCFLMLLCYVVAKKKT---EDHKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVK 278
+LV F LL + + E KVK+ +YYESLCP + FI L ++ D +
Sbjct: 12 KLVFFACLLLFTFSSHNLVAGESDKVKLNLYYESLCPSCQNFIVHHLGKIFNTDLHTITD 71
Query: 279 VKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQT 458
+K++P+G + V + C HG +EC N ++AC ++ Q + + C+ T
Sbjct: 72 LKLIPFGNA---HVSDDLTVTCQHGEEECKLNALEACAIRTWPNQRLH-YKFIRCVETNT 127
Query: 459 SPDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIIN 632
+ + ++C V K + C + + L+ Y ++T ++ +VP + +N
Sbjct: 128 N---AWESC---VKKYGGEKAINDCYNGDLSKELILGYANQTLSLKPEHKYVPWMTLN 179
>At5g01580.1 68418.m00073 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 61.3 bits (142), Expect = 7e-10
Identities = 42/179 (23%), Positives = 78/179 (43%), Gaps = 1/179 (0%)
Frame = +3
Query: 99 MKTLRLVCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLV 275
M + + +C + + KV +++YYE+LCP +FI +L ++ +
Sbjct: 1 MASYQRLCITSCTIFFCLLSLSSSQKVTLSLYYEALCPFCAEFIVNRLPKIFETGLISSI 60
Query: 276 KVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQ 455
+++VP+G + + DG + +C HG EC N + AC + D K I Q
Sbjct: 61 DLQLVPWGNAA-IRPDG--TILCQHGEAECALNAIHACAI--NAYPDVMKHFGYIYCTEQ 115
Query: 456 TSPDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIIN 632
+ L+ ++ + S C + G+ L Y ++T + FVP V++N
Sbjct: 116 LVLENKLEKWADCLEMVGLSRAAVDCYINGYGNQLEQRYAEETSELYPAHRFVPWVVVN 174
>At2g44580.1 68415.m05548 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 624
Score = 37.5 bits (83), Expect = 0.010
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = +3
Query: 315 KVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTSPDKSLDTCLTQ 494
++DG W I + D + CVLKD + D ++ E+V L+ P + CL
Sbjct: 194 EIDGFWRVIDENYLDVILRMLLHNCVLKDWSFDDLDEDEVVNALVADEFPSQLASHCLRV 253
Query: 495 V-DKMSESDKLK 527
K++E+DK K
Sbjct: 254 FGSKVNETDKWK 265
>At3g23980.1 68416.m03012 dentin sialophosphoprotein-related
contains weak similarity to Dentin sialophosphoprotein
precursor (Swiss-Prot:Q9NZW4) [Homo sapiens]
Length = 736
Score = 30.3 bits (65), Expect = 1.5
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1
Query: 250 SGGTSEDLSKSKWSRMGRARTTRWMGNG 333
SGG S DLSK+ SR + +T +GNG
Sbjct: 525 SGGKSTDLSKNSTSRKNVSTSTEGLGNG 552
>At4g38350.1 68417.m05422 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (PID:g2276463); contains Pfam profile PF02460
Patched family
Length = 1064
Score = 29.9 bits (64), Expect = 2.0
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +3
Query: 117 VCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR 257
V ++ LC + K E K+ V ES + KKF T L+P +R
Sbjct: 315 VAIVLALCSGLYNFKVETRPEKLWVGPESKAAEEKKFFDTHLSPFYR 361
>At1g42470.1 68414.m04897 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (GB:AAB63982) (GI:2276463); contains Pfam
profile PF02460 Patched family
Length = 1272
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +3
Query: 117 VCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR 257
V ++LLC + + K E K+ V S + K+F T LAP +R
Sbjct: 356 VSVVLLLCVGLIRFKVETRPDKLWVGSGSRAAEEKQFFDTHLAPFYR 402
>At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family
protein
Length = 846
Score = 29.5 bits (63), Expect = 2.6
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +2
Query: 377 GASLRPEGPQPPGHGEDGDCNMPHEPDQPRQ 469
G S+ P GP PP H G P+ P +Q
Sbjct: 776 GYSIPPYGPPPPYHTPHGQAPQPYPPQAQQQ 806
>At5g43130.1 68418.m05265 transcription initiation factor IID
(TFIID) component TAF4 family protein weak similarity to
SP|O00268 Transcription initiation factor TFIID 135 kDa
subunit {Homo sapiens}; contains Pfam profile PF05236:
Transcription initiation factor TFIID component TAF4
family
Length = 712
Score = 29.1 bits (62), Expect = 3.4
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +3
Query: 501 KMSESDKLKRCASSEQGDNLLASYGDKTD 587
K +E++KLK+ + SE+GD + S DK D
Sbjct: 556 KQAEAEKLKKPSESEEGDGGVDSEKDKED 584
>At4g03420.1 68417.m00469 expressed protein
Length = 310
Score = 28.7 bits (61), Expect = 4.5
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +3
Query: 435 VICLMNQTSPDKSLDTCLTQVDKMSESDKLKRCASSE 545
+I L + + +S D+ SESDKL RCAS E
Sbjct: 97 LIRLRDDSEDGESRDSFSDSYSDESESDKLSRCASDE 133
>At1g06490.1 68414.m00688 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1933
Score = 28.7 bits (61), Expect = 4.5
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 7/68 (10%)
Frame = +3
Query: 135 LCYVVAKKKTEDHKVKIA-VY------YESLCPDSKKFITTQLAPVWRDFRGLVKVKMVP 293
LCY+ E H + VY YE+ PD + F+ + P+++ R +V+
Sbjct: 363 LCYIFHNMANEVHGILFGNVYPVTGDTYEAGAPDEEAFLRNVITPIYQVLR--KEVRRNK 420
Query: 294 YGKSTHDK 317
GK++H K
Sbjct: 421 NGKASHSK 428
>At3g59140.1 68416.m06593 ABC transporter family protein putative
multi resistance protein mrp - Arabidopsis thaliana,
EMBL:ATMRPPROT
Length = 1453
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = +3
Query: 411 QDTEKMEIVICLMNQTSPDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASY 572
++T E V+ + N T P K ++ ++ K+ + +L + E+GD L Y
Sbjct: 827 RETAGSERVVAVENPTKPVKEINRVISSQSKVLKPSRLIKQEEREKGDTGLRPY 880
>At1g60180.1 68414.m06779 hypothetical protein similar to
hypothetical protein GI:6017113 from [Arabidopsis
thaliana]
Length = 296
Score = 28.3 bits (60), Expect = 6.0
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 590 SIRFITVRREQIVSLFTGCASFQFIRLRHLVYLS 489
S+RF + E I + +GC + + L H +YL+
Sbjct: 16 SLRFCELSDECIAKILSGCPILESLTLSHCIYLT 49
>At5g38260.1 68418.m04612 serine/threonine protein kinase, putative
similar to receptor serine/threonine kinase PR55K
gi|1235680|gb|AAC49208; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 638
Score = 27.9 bits (59), Expect = 7.9
Identities = 24/86 (27%), Positives = 37/86 (43%), Gaps = 5/86 (5%)
Frame = +3
Query: 219 KKFITTQLAPVWRDFRGLVKVKMVPYGK-----STHDKVDGKWSFICHHGADECYGNKVQ 383
K+ + L P + +GLV++K Y + GK F +G + C G KV
Sbjct: 288 KRRTSHHLRPRDNNLKGLVQLKQYSYAEVRKITKLFSHTLGKGGFGTVYGGNLCDGRKVA 347
Query: 384 ACVLKDRNLQDTEKMEIVICLMNQTS 461
+LKD + E + M+QTS
Sbjct: 348 VKILKDFK-SNGEDFINEVASMSQTS 372
>At2g37730.1 68415.m04627 fringe-related protein similarity to
predicted proteins + similar to hypothetical protein
GB:AAC23643 [Arabidopsis thaliana] + weak similarity to
Fringe [Schistocerca gregaria](GI:6573138);Fringe
encodes an extracellular protein that regulates Notch
signalling.
Length = 532
Score = 27.9 bits (59), Expect = 7.9
Identities = 17/48 (35%), Positives = 21/48 (43%)
Frame = -1
Query: 591 QHPFYHRTTRADCLLVHWMRIFSVYPTPTSCLLELSTCPKTCRGWSGS 448
QH F H TR + V W +YPT + EL T T + W S
Sbjct: 352 QHSFCHDQTRNWYVSVSWGYTIQIYPTLVTA-KELETPFLTFKSWRTS 398
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,539,690
Number of Sequences: 28952
Number of extensions: 379279
Number of successful extensions: 1170
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1162
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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