BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10a05 (614 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 92 5e-20 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 82 7e-17 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 58 8e-10 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 50 4e-07 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 1e-05 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 33 0.025 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 33 0.043 SPAC25G10.01 ||SPAC2C4.18|RNA-binding protein|Schizosaccharomyce... 32 0.076 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.17 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.40 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 29 0.53 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 27 2.2 SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharo... 27 2.8 SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain p... 26 3.8 SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch... 25 8.7 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 92.3 bits (219), Expect = 5e-20 Identities = 48/144 (33%), Positives = 77/144 (53%) Frame = +1 Query: 118 LTNAQEQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDTGKL 297 LT+ Q + REAF+L D +G I + + V +++LG PT ELQ MI VD G + Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65 Query: 298 SFENFETAIMRKIMALDSDGDIMKSFRLFDDDDSGFINFENVKKVSQILGTYLTXXXXXX 477 F F T + RK+ D++ ++ ++F++FD D +G+I E + V LG L+ Sbjct: 66 DFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125 Query: 478 XXXXXXXXFDGLISVEEFMKMIKN 549 DG+I+ EEF ++I + Sbjct: 126 MIREADTDGDGVINYEEFSRVISS 149 Score = 48.0 bits (109), Expect = 1e-06 Identities = 23/67 (34%), Positives = 35/67 (52%) Frame = +1 Query: 133 EQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDTGKLSFENF 312 E+++REAF + D G G I E+ + +LG + EE+ MIR D G +++E F Sbjct: 84 EEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143 Query: 313 ETAIMRK 333 I K Sbjct: 144 SRVISSK 150 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 81.8 bits (193), Expect = 7e-17 Identities = 43/145 (29%), Positives = 75/145 (51%) Frame = +1 Query: 109 KLTLTNAQEQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDT 288 ++ +T Q QD+ EAF L D + I + R A++ALG+ E+ ++R DK Sbjct: 28 RVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGK 87 Query: 289 GKLSFENFETAIMRKIMALDSDGDIMKSFRLFDDDDSGFINFENVKKVSQILGTYLTXXX 468 G L E+F + KI+ D +I ++F LFDDD++G I+ N+++V++ L + Sbjct: 88 GYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQE 147 Query: 469 XXXXXXXXXXXFDGLISVEEFMKMI 543 DG I+ +EF+ ++ Sbjct: 148 LEAMIEEFDLDQDGEINEQEFIAIM 172 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 58.4 bits (135), Expect = 8e-10 Identities = 34/131 (25%), Positives = 66/131 (50%) Frame = +1 Query: 55 SNSEEDLNQSSKVNEIKAKLTLTNAQEQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYE 234 ++S+ Q++K A LT++Q Q+L+EAF LLD G+G I ED + + +L + Sbjct: 21 TSSQRVAAQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQD 80 Query: 235 PTNEELQTMIRAVDKGDTGKLSFENFETAIMRKIMALDSDGDIMKSFRLFDDDDSGFINF 414 + + + M +++ ++ F TA+ + + D++++F FDD SG I Sbjct: 81 ASEDSINHMFESINP----PINLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKIPI 136 Query: 415 ENVKKVSQILG 447 ++ +G Sbjct: 137 STMRDALSSMG 147 Score = 33.5 bits (73), Expect = 0.025 Identities = 20/62 (32%), Positives = 32/62 (51%) Frame = +1 Query: 139 DLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDTGKLSFENFET 318 DL EAF+ D T GKI R A+ ++G +E+++++R+ G +E F Sbjct: 118 DLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYT--SHGVFYYEKFVD 175 Query: 319 AI 324 AI Sbjct: 176 AI 177 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 49.6 bits (113), Expect = 4e-07 Identities = 30/110 (27%), Positives = 55/110 (50%) Frame = +1 Query: 130 QEQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDTGKLSFEN 309 Q +++EAF L D +G I +++LG T+ EL + + GD + + Sbjct: 7 QTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDA--IDEKK 62 Query: 310 FETAIMRKIMALDSDGDIMKSFRLFDDDDSGFINFENVKKVSQILGTYLT 459 F + + K+ +S+ + +K+FR+FD D+SG+I + LG L+ Sbjct: 63 FMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLS 112 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 44.4 bits (100), Expect = 1e-05 Identities = 34/143 (23%), Positives = 62/143 (43%), Gaps = 3/143 (2%) Frame = +1 Query: 124 NAQEQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDTGKLSF 303 + + ++AF+L D G G+I ++A G PT E I ++ ++ Sbjct: 2 STDDSPYKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDM 57 Query: 304 ENFETAIMRKIMALDSDGD---IMKSFRLFDDDDSGFINFENVKKVSQILGTYLTXXXXX 474 E F ++ + D GD +K F++FD D +G I ++ V LG L+ Sbjct: 58 EQF-LQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMD 116 Query: 475 XXXXXXXXXFDGLISVEEFMKMI 543 DG+++ +F++MI Sbjct: 117 ELLKGVPVK-DGMVNYHDFVQMI 138 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 33.5 bits (73), Expect = 0.025 Identities = 30/100 (30%), Positives = 42/100 (42%), Gaps = 3/100 (3%) Frame = +1 Query: 124 NAQEQDLREAFNLLDYTGEGKIRAEDFRVAIKALGYEPTNEELQTMIRAVDKGDTGKLSF 303 NA + L E F D GK+ E+F+ + L T E+ + + G + K+S Sbjct: 329 NASMEFLEETFQKADADHSGKLSFEEFQHFVSLL---KTRSEIVDIFKEYTSG-SDKMSL 384 Query: 304 ENFETAIMRKIMA-LDSDG--DIMKSFRLFDDDDSGFINF 414 E F + A LDSD + SF DD G I F Sbjct: 385 EQFRHFLSTSQKARLDSDSIRTLYVSFCSNDDSKMGLIEF 424 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 32.7 bits (71), Expect = 0.043 Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 5/70 (7%) Frame = +1 Query: 130 QEQDLREAFNLLDYTGEGKIRAEDFRVAIKAL-GYEPTNEELQTM----IRAVDKGDTGK 294 +E+ L+ AF + D +G I + + +K + G ++LQ + I VDK GK Sbjct: 91 KEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGK 150 Query: 295 LSFENFETAI 324 +SFE F+ + Sbjct: 151 ISFEEFKDIV 160 >SPAC25G10.01 ||SPAC2C4.18|RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 31.9 bits (69), Expect = 0.076 Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 6/65 (9%) Frame = +3 Query: 231 RAN*RGAADNDKSGGQRR--HREIELRKFRNGHN----EKDHGPGQRRRYHEELPTLR*R 392 R N R N+K GG RR +R+ + ++RN + +++H PG R+ + + R Sbjct: 196 RRNSRDFPSNNKDGGYRRNNYRDRDSNRYRNSYRPSRPQREHSPGNYRKERYNVDSRPRR 255 Query: 393 *QWFH 407 + FH Sbjct: 256 ERHFH 260 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 30.7 bits (66), Expect = 0.17 Identities = 19/66 (28%), Positives = 33/66 (50%) Frame = +1 Query: 343 LDSDGDIMKSFRLFDDDDSGFINFENVKKVSQILGTYLTXXXXXXXXXXXXXXFDGLISV 522 L+ D + ++F LFD G+I+FE++++ LG LT +G +S Sbjct: 8 LEMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGT--NGKVSR 65 Query: 523 EEFMKM 540 EEF ++ Sbjct: 66 EEFAEL 71 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 29.5 bits (63), Expect = 0.40 Identities = 19/65 (29%), Positives = 34/65 (52%) Frame = +1 Query: 250 LQTMIRAVDKGDTGKLSFENFETAIMRKIMALDSDGDIMKSFRLFDDDDSGFINFENVKK 429 L+ + DK TG LS ++ + I ++ D +I F L+D + GF++ +V K Sbjct: 581 LRHLFLRFDKSMTGSLSLQDLVSGIA-ELKFRDVMRNISFIFELYDFNGDGFMDKPDVLK 639 Query: 430 VSQIL 444 VS+ + Sbjct: 640 VSEAI 644 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 29.1 bits (62), Expect = 0.53 Identities = 21/67 (31%), Positives = 35/67 (52%), Gaps = 2/67 (2%) Frame = +1 Query: 118 LTNAQEQDLREAFNLLDYTGEGKIRAEDFRVAIKAL--GYEPTNEELQTMIRAVDKGDTG 291 LTN + L + FN LD G+G + D IKA + + +E++ IR V+ +G Sbjct: 13 LTNEEILTLTDQFNKLDVDGKGYL---DQPTTIKAFEDSKKGSYDEVREAIREVNVDSSG 69 Query: 292 KLSFENF 312 ++ E+F Sbjct: 70 RVEPEDF 76 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 27.1 bits (57), Expect = 2.2 Identities = 10/36 (27%), Positives = 25/36 (69%) Frame = +1 Query: 43 TIAYSNSEEDLNQSSKVNEIKAKLTLTNAQEQDLRE 150 TI + S+ D+ ++ +N++K+++T+T+ +DL + Sbjct: 269 TIRHLESQSDIINTT-INDLKSQMTITDESSEDLEK 303 >SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 397 Score = 26.6 bits (56), Expect = 2.8 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = -2 Query: 586 HRQNPTEFLCEHYFLSFS*ILRPISVRRNLYRHRQSFLQ 470 H NP++ L EH F++ I + ++ L+R ++ Q Sbjct: 186 HSHNPSQLLFEHPFMALGLIFGSVVLKEWLFRKTRTVAQ 224 >SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 607 Score = 26.2 bits (55), Expect = 3.8 Identities = 25/91 (27%), Positives = 44/91 (48%) Frame = +1 Query: 37 SGTIAYSNSEEDLNQSSKVNEIKAKLTLTNAQEQDLREAFNLLDYTGEGKIRAEDFRVAI 216 +G +AYS + ++S+K + + + TLT A+E++ +EA L K R ++ Sbjct: 520 AGGVAYSEYVKQNSKSAKKTKKRQRDTLT-AEEKEEKEAKELSKMMMSNKQRKLYSKLKN 578 Query: 217 KALGYEPTNEELQTMIRAVDKGDTGKLSFEN 309 + E N L+ R ++K KL EN Sbjct: 579 ENSKNENYNNALRNRKRDIEK--RKKLKVEN 607 >SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1031 Score = 25.0 bits (52), Expect = 8.7 Identities = 13/54 (24%), Positives = 28/54 (51%), Gaps = 1/54 (1%) Frame = +1 Query: 274 DKGDTGKLSFENFETAIMRKIMALDSDGDIMKSFRLF-DDDDSGFINFENVKKV 432 D +TG L+ E+ + + + + + D+ + + DDDD+ FE+V ++ Sbjct: 185 DLDETGNLAPEDLKDEV-KSVHEFNEPNDLRQQEESYSDDDDTNVNEFEDVNEI 237 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,226,317 Number of Sequences: 5004 Number of extensions: 41350 Number of successful extensions: 173 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 170 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 269634532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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