BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmte10a04 (708 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharo... 72 7e-14 SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 48 1e-06 SPBC13G1.06c |isd11||iron sulfur cluster assembly protein Isd11|... 36 0.006 SPAC589.11 |mug82||translation release factor |Schizosaccharomyc... 28 1.5 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 27 2.0 SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 26 4.6 SPBC13G1.11 |ykt6||SNARE Ykt6|Schizosaccharomyces pombe|chr 2|||... 26 6.1 SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 8.0 SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 25 8.0 >SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 162 Score = 72.1 bits (169), Expect = 7e-14 Identities = 31/98 (31%), Positives = 63/98 (64%), Gaps = 2/98 (2%) Frame = +2 Query: 419 KVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARE 598 ++ ++ E ++ E F+ G GPGG +NK S + HIPTG +++ +R ++ NR AR+ Sbjct: 43 QLERLQEEDIEETFICGKGPGGQKINKTSIVAQVKHIPTGIIVRSQDTRSREQNRCIARK 102 Query: 599 LLIEKLDDMINGPESVSAQR--KLIEEKKYKRNATKKQ 706 L EK+D+ +G +S+ A++ +++++KK + +K++ Sbjct: 103 RLTEKVDEFKHGNDSLLARKVQRIVKKKKQREKKSKRK 140 >SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 48.4 bits (110), Expect = 1e-06 Identities = 26/72 (36%), Positives = 39/72 (54%) Frame = +2 Query: 443 ELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIEKLDD 622 E+ + +R G GG VN+ + V LTHIPTG + SR Q N++KA +L +L Sbjct: 256 EVKIEVMRSRGAGGQHVNRTESAVRLTHIPTGITVSMQDSRSQHQNKEKAFLVLNSRL-A 314 Query: 623 MINGPESVSAQR 658 +N + A+R Sbjct: 315 ALNAAKENEAER 326 >SPBC13G1.06c |isd11||iron sulfur cluster assembly protein Isd11|Schizosaccharomyces pombe|chr 2|||Manual Length = 102 Score = 35.9 bits (79), Expect = 0.006 Identities = 22/70 (31%), Positives = 38/70 (54%), Gaps = 3/70 (4%) Frame = +1 Query: 133 MNITRKEVLRLYRNLLMYSKSLKLTDPDYFRRSIKIEFKNNQNLTDLEKISFAYQKGQAL 312 M+++++ V+RLYRN+L SK T +Y R + +FK + +D K + + L Sbjct: 1 MSVSKQHVVRLYRNILKTSKLFPYTYREYTIRRTRDKFKELKVESDPAKFEQGIKDSEKL 60 Query: 313 L---QRGSVV 333 L QR S++ Sbjct: 61 LEIIQRQSII 70 >SPAC589.11 |mug82||translation release factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 182 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = +2 Query: 458 FVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKC 553 F R SGPGG VNK N V+ ++P + C Sbjct: 53 FSRSSGPGGQNVNK-LNTKVIVNLPFKQLESC 83 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = +2 Query: 584 KKARELLIEK-LDDMINGPESVSAQRKLIEEKKYKRNATKKQ 706 K+ E++++ +D+ + ES+SA RK + K Y + T+KQ Sbjct: 575 KQLYEVILQAVIDNRVVARESISALRKNVTAKCYGGDVTRKQ 616 >SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy |Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 26.2 bits (55), Expect = 4.6 Identities = 14/53 (26%), Positives = 23/53 (43%) Frame = +2 Query: 173 IC*CTRNH*NSPTQIIFGAQLKSNSKITKT*LIWRKLASRIRKVRPCCREGQL 331 +C R+ +P++ + G+Q + SK+ T W S V CR L Sbjct: 64 LCDFERSQILAPSECVRGSQSECVSKLESTSTWWLSFTSHFHDVNHLCRLANL 116 >SPBC13G1.11 |ykt6||SNARE Ykt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 197 Score = 25.8 bits (54), Expect = 6.1 Identities = 10/29 (34%), Positives = 19/29 (65%) Frame = +2 Query: 608 EKLDDMINGPESVSAQRKLIEEKKYKRNA 694 EKLDD+I +++S Q ++ + K+N+ Sbjct: 164 EKLDDLIQRSDNLSTQSRMFYKSAKKQNS 192 >SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 840 Score = 25.4 bits (53), Expect = 8.0 Identities = 11/32 (34%), Positives = 22/32 (68%), Gaps = 1/32 (3%) Frame = +1 Query: 184 YSKSLKLTDPDYFRRSIKIEFKN-NQNLTDLE 276 YS+++ L + + F R I IEF++ + +L D++ Sbjct: 408 YSRTVTLDEMEQFTREISIEFRSISSSLRDVD 439 >SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 25.4 bits (53), Expect = 8.0 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 398 KHTIDYSKVPKINECELSEKFVRGSGPG 481 ++TI SK+PK+ E E +K + G G Sbjct: 221 QNTIYESKIPKLIEHETIQKIAKSKGEG 248 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,886,108 Number of Sequences: 5004 Number of extensions: 58979 Number of successful extensions: 168 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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