BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov12g09 (540 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosa... 29 0.58 SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.0 SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Sc... 25 5.4 SPAC8F11.10c |pvg1|SPACUNK4.18|pyruvyltransferase |Schizosacchar... 25 9.5 SPAC1D4.07c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 9.5 >SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosaccharomyces pombe|chr 1|||Manual Length = 1162 Score = 28.7 bits (61), Expect = 0.58 Identities = 20/68 (29%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = -2 Query: 299 LLYYLYRYNFLVSVLF-YNKIICKRVLLILFEI*LNVFVENALPIACIGENLHNYSSLNS 123 L Y LY N L L Y + + IL++ FVEN + + + + N S L Sbjct: 801 LCYQLYEDNELTDALNNYLDLFGIKFAFILYDY----FVENGMALELLNSDRFNKSYLKQ 856 Query: 122 FFYAREHH 99 FF +R+++ Sbjct: 857 FFKSRDYN 864 >SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 27.9 bits (59), Expect = 1.0 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = -2 Query: 161 IGENLHNYSSLNSFFYARE 105 I E LH++S L+SFFYA + Sbjct: 45 INEMLHSHSELSSFFYAEK 63 >SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Schizosaccharomyces pombe|chr 2|||Manual Length = 300 Score = 25.4 bits (53), Expect = 5.4 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = -2 Query: 527 KAPSXDVK*TIISSENITTARESTLRGILLLGILSWFH 414 KAP+ + T+++ NITT +T + +L + L H Sbjct: 156 KAPNVLTQPTMLNERNITTQENNTTKNVLHISRLLQAH 193 >SPAC8F11.10c |pvg1|SPACUNK4.18|pyruvyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 24.6 bits (51), Expect = 9.5 Identities = 9/26 (34%), Positives = 16/26 (61%) Frame = +3 Query: 432 PQKQYSPKSTLSCSSNIFTTYYSLFY 509 P+ S +ST++ SN+ TYY ++ Sbjct: 62 PRNSASCESTITLQSNLLFTYYKHYF 87 >SPAC1D4.07c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 146 Score = 24.6 bits (51), Expect = 9.5 Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Frame = -2 Query: 308 ISVLLYYLYRYNFLVSVLFYNKIICKRVLLILF-EI*LNVFVENALPI 168 +S+L YY Y+ +SVL K+ + V + +F + N+F+ ++ I Sbjct: 33 LSILPYYKNSYSDFISVLQMYKVHIRMVSIYIFLKALSNIFLASSCEI 80 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,678,077 Number of Sequences: 5004 Number of extensions: 25637 Number of successful extensions: 67 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 65 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 67 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 221892220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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