BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov12f03 (443 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g37720.1 68415.m04625 expressed protein 28 3.3 At4g00290.1 68417.m00036 mechanosensitive ion channel domain-con... 27 4.3 At4g00234.1 68417.m00028 expressed protein 27 5.7 At5g14570.2 68418.m01709 transporter, putative similar to trans-... 27 7.5 At5g14570.1 68418.m01708 transporter, putative similar to trans-... 27 7.5 At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 26 10.0 >At2g37720.1 68415.m04625 expressed protein Length = 482 Score = 27.9 bits (59), Expect = 3.3 Identities = 21/60 (35%), Positives = 32/60 (53%) Frame = +1 Query: 61 GVRIPQAGTNFSNEIRTQQMFTIDFHGEGITSCNKNQTRNIIICVITGGRTSCESARVGT 240 G IP+ N N +R Q TI F G+ S + Q ++++ C+ TGG+ S E VG+ Sbjct: 193 GCNIPEF--NRVNFLRRMQNKTIAFIGD---SLGREQFQSLM-CMATGGKESPEVQNVGS 246 >At4g00290.1 68417.m00036 mechanosensitive ion channel domain-containing protein / MS ion channel domain-containing protein weak similarity to SP|P77338 Potassium efflux system kefA {Escherichia coli}; contains Pfam profile PF00924: Mechanosensitive ion channel Length = 497 Score = 27.5 bits (58), Expect = 4.3 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -1 Query: 260 AEIGGVVVPTRADSQEVLPPVITQIIILRVWFL 162 A+I +V PT +Q P V +I+ VWFL Sbjct: 223 AQIAAMVAPTTIAAQYFSPTVKGAVILSLVWFL 255 >At4g00234.1 68417.m00028 expressed protein Length = 263 Score = 27.1 bits (57), Expect = 5.7 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -1 Query: 260 AEIGGVVVPTRADSQEVLPPVITQIIILRVWFL 162 A+I +V PT +Q P V +I+ VWFL Sbjct: 171 AQIAAMVAPTTIAAQYFSPTVKGAVILSVVWFL 203 >At5g14570.2 68418.m01709 transporter, putative similar to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI:3608362; contains Pfam profile PF00083: Sugar (and other) transporter Length = 397 Score = 26.6 bits (56), Expect = 7.5 Identities = 13/46 (28%), Positives = 26/46 (56%) Frame = -3 Query: 189 NYNIAGLVFITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 52 NY A ++ + YS+ VE+ +++++ YF + G L + T A+ Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311 >At5g14570.1 68418.m01708 transporter, putative similar to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI:3608362; contains Pfam profile PF00083: Sugar (and other) transporter Length = 493 Score = 26.6 bits (56), Expect = 7.5 Identities = 13/46 (28%), Positives = 26/46 (56%) Frame = -3 Query: 189 NYNIAGLVFITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 52 NY A ++ + YS+ VE+ +++++ YF + G L + T A+ Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311 >At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI:7108523; E3, HECT-domain protein family; similar to ubiquitin-protein ligase 2 GI:7108523 from [Arabidopsis thaliana] Length = 3658 Score = 26.2 bits (55), Expect = 10.0 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +2 Query: 74 RRRVPIFLMKYVLNKCSRLTST 139 ++RVP +M+ VL C+RLT + Sbjct: 1564 KQRVPAMIMQAVLQLCARLTKS 1585 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,379,186 Number of Sequences: 28952 Number of extensions: 217306 Number of successful extensions: 404 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 400 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 404 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 712739520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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