Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmov12f03
         (443 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g37720.1 68415.m04625 expressed protein                             28   3.3  
At4g00290.1 68417.m00036 mechanosensitive ion channel domain-con...    27   4.3  
At4g00234.1 68417.m00028 expressed protein                             27   5.7  
At5g14570.2 68418.m01709 transporter, putative similar to trans-...    27   7.5  
At5g14570.1 68418.m01708 transporter, putative similar to trans-...    27   7.5  
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    26   10.0 

>At2g37720.1 68415.m04625 expressed protein 
          Length = 482

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 21/60 (35%), Positives = 32/60 (53%)
 Frame = +1

Query: 61  GVRIPQAGTNFSNEIRTQQMFTIDFHGEGITSCNKNQTRNIIICVITGGRTSCESARVGT 240
           G  IP+   N  N +R  Q  TI F G+   S  + Q ++++ C+ TGG+ S E   VG+
Sbjct: 193 GCNIPEF--NRVNFLRRMQNKTIAFIGD---SLGREQFQSLM-CMATGGKESPEVQNVGS 246


>At4g00290.1 68417.m00036 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein weak similarity to SP|P77338
           Potassium efflux system kefA {Escherichia coli};
           contains Pfam profile PF00924: Mechanosensitive ion
           channel
          Length = 497

 Score = 27.5 bits (58), Expect = 4.3
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = -1

Query: 260 AEIGGVVVPTRADSQEVLPPVITQIIILRVWFL 162
           A+I  +V PT   +Q   P V   +I+  VWFL
Sbjct: 223 AQIAAMVAPTTIAAQYFSPTVKGAVILSLVWFL 255


>At4g00234.1 68417.m00028 expressed protein
          Length = 263

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = -1

Query: 260 AEIGGVVVPTRADSQEVLPPVITQIIILRVWFL 162
           A+I  +V PT   +Q   P V   +I+  VWFL
Sbjct: 171 AQIAAMVAPTTIAAQYFSPTVKGAVILSVVWFL 203


>At5g14570.2 68418.m01709 transporter, putative similar to
           trans-membrane nitrate transporter protein AtNRT2:1
           [Arabidopsis thaliana] GI:3747058, high-affinity nitrate
           transporter ACH1 [Arabidopsis thaliana] GI:3608362;
           contains Pfam profile PF00083: Sugar (and other)
           transporter
          Length = 397

 Score = 26.6 bits (56), Expect = 7.5
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = -3

Query: 189 NYNIAGLVFITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 52
           NY  A ++ +   YS+ VE+  +++++ YF  + G  L  + T A+
Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311


>At5g14570.1 68418.m01708 transporter, putative similar to
           trans-membrane nitrate transporter protein AtNRT2:1
           [Arabidopsis thaliana] GI:3747058, high-affinity nitrate
           transporter ACH1 [Arabidopsis thaliana] GI:3608362;
           contains Pfam profile PF00083: Sugar (and other)
           transporter
          Length = 493

 Score = 26.6 bits (56), Expect = 7.5
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = -3

Query: 189 NYNIAGLVFITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 52
           NY  A ++ +   YS+ VE+  +++++ YF  + G  L  + T A+
Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
            identical to ubiquitin-protein ligase 2 [Arabidopsis
            thaliana] GI:7108523; E3, HECT-domain protein family;
            similar to ubiquitin-protein ligase 2 GI:7108523 from
            [Arabidopsis thaliana]
          Length = 3658

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +2

Query: 74   RRRVPIFLMKYVLNKCSRLTST 139
            ++RVP  +M+ VL  C+RLT +
Sbjct: 1564 KQRVPAMIMQAVLQLCARLTKS 1585


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,379,186
Number of Sequences: 28952
Number of extensions: 217306
Number of successful extensions: 404
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 400
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 404
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)