BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov12d20 (506 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe... 27 1.6 SPAC105.01c |||potassium ion/proton antiporter|Schizosaccharomyc... 27 2.1 SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 27 2.1 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 4.9 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 8.6 >SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 27.1 bits (57), Expect = 1.6 Identities = 9/33 (27%), Positives = 18/33 (54%) Frame = +3 Query: 270 RQRYFKYLAFRSRLIVEIGRRENSFNFKQCFFL 368 RQ + LA ++++ + + +FN + CF L Sbjct: 486 RQEQMRLLAVLEQVLINVAKNTPAFNLQSCFLL 518 >SPAC105.01c |||potassium ion/proton antiporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 898 Score = 26.6 bits (56), Expect = 2.1 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +1 Query: 40 IASFHRSNGGWLVASRIPFTGKQNLKISRRVED 138 I H GG+LV + IP +KI+ ++ED Sbjct: 280 IIGIHPIFGGFLVGTIIPHENDLTVKITEKIED 312 >SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 362 Score = 26.6 bits (56), Expect = 2.1 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = -1 Query: 371 LQKKTLFKIKTVFTPAYFHYKSAPERKVFEISLPIVSVPRK 249 L ++T+ ++KT A F +P KV S PIV++ +K Sbjct: 59 LPERTVERLKTECNAALFGAVQSPTHKVAGYSSPIVALRKK 99 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.4 bits (53), Expect = 4.9 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 3/34 (8%) Frame = -1 Query: 326 AYFHYKSAPERKVFEISLPIVSVPR---KPCLPT 234 AY ++PE KVF+ +P+ P+ KP PT Sbjct: 350 AYVAKSTSPELKVFDTVMPVALSPKPAQKPPSPT 383 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 24.6 bits (51), Expect = 8.6 Identities = 11/17 (64%), Positives = 12/17 (70%) Frame = +3 Query: 297 FRSRLIVEIGRRENSFN 347 F SRL VE G+ EN FN Sbjct: 801 FLSRLNVESGKAENKFN 817 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,035,280 Number of Sequences: 5004 Number of extensions: 39863 Number of successful extensions: 111 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 111 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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