Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov12c21
(571 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 46 2e-05
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 45 3e-05
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 45 4e-05
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 44 7e-05
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 44 9e-05
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 44 9e-05
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 43 2e-04
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 38 0.005
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 33 0.18
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 2.2
At1g20410.1 68414.m02545 expressed protein 28 3.8
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 5.0
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 6.7
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 8.8
At1g20740.1 68414.m02598 expressed protein ; expression supporte... 27 8.8
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 45.6 bits (103), Expect = 2e-05
Identities = 42/163 (25%), Positives = 75/163 (46%), Gaps = 8/163 (4%)
Frame = +3
Query: 84 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMAMANRDVGSTI 263
MSL+P I G + + D +L D +TP+ + A N V
Sbjct: 1 MSLIPSIFGGRRTNVFDP---FSLDVFDPFEGFLTPSGLANA-PAMDVAAFTNAKVDWRE 56
Query: 264 TSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTR 419
T F+ D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTR
Sbjct: 57 TPEAHVFKA--DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTR 114
Query: 420 RYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 548
R+ LPEN + +++ + +GVL+V P+ P +++ I+
Sbjct: 115 RFRLPENAKMEEIKASM-ENGVLSVTVPKVPEKKPEVKSIDIS 156
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 45.2 bits (102), Expect = 3e-05
Identities = 31/95 (32%), Positives = 49/95 (51%), Gaps = 8/95 (8%)
Frame = +3
Query: 297 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 452
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 453 TVESRLSSDGVLTVIAPRTPAATKNERAVPITQTG 557
V++ + +GVLTV P+ A K + I +G
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKKKAQVKSIDISG 157
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 44.8 bits (101), Expect = 4e-05
Identities = 20/91 (21%), Positives = 45/91 (49%), Gaps = 3/91 (3%)
Frame = +3
Query: 261 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYAL 431
I + + ++ D+ S E++ + D ++++G+ ++ + + R + R L
Sbjct: 131 IKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQL 190
Query: 432 PENCNPDTVESRLSSDGVLTVIAPRTPAATK 524
P+NC D +++ L +GVL + P+T K
Sbjct: 191 PDNCEKDKIKAEL-KNGVLFITIPKTKVERK 220
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 44.0 bits (99), Expect = 7e-05
Identities = 27/92 (29%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +3
Query: 297 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 452
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 453 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 548
V++ + +GVLTV+ P+ P +++ I+
Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVKSIDIS 158
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 43.6 bits (98), Expect = 9e-05
Identities = 44/164 (26%), Positives = 75/164 (45%), Gaps = 9/164 (5%)
Frame = +3
Query: 84 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMA-MANRDVGST 260
MSL+P G + + D +L D +TP ++ P K +A N V
Sbjct: 1 MSLVPSFFGGRRTNVFDP---FSLDVWDPFEGFLTPGLTN---APAKDVAAFTNAKVDWR 54
Query: 261 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFT 416
T F+ DV EE+ V+ DG ++ + G+ +EE+ D + R +F
Sbjct: 55 ETPEAHVFKA--DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFM 112
Query: 417 RRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 548
RR+ LPEN + V++ + +GVL+V P+ + ++V I+
Sbjct: 113 RRFRLPENAKVEEVKASM-ENGVLSVTVPKVQESKPEVKSVDIS 155
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 43.6 bits (98), Expect = 9e-05
Identities = 28/92 (30%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +3
Query: 297 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 452
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 453 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 548
V++ + +GVLTV P+ + +++ I+
Sbjct: 122 QVKAAM-ENGVLTVTVPKAETKKADVKSIQIS 152
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 42.7 bits (96), Expect = 2e-04
Identities = 35/117 (29%), Positives = 56/117 (47%), Gaps = 8/117 (6%)
Frame = +3
Query: 231 AMANRDVGSTITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHG 395
A+AN V T+ F+ D+ EE+ V+ D V+ + G+ EE+QD
Sbjct: 42 AIANARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 99
Query: 396 YISRQ---FTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTG 557
+ R F+R++ LPEN D V++ + +GVLTV P+ K + I +G
Sbjct: 100 RVERSSGGFSRKFRLPENVKMDQVKASM-ENGVLTVTVPKVETNKKKAQVKSIDISG 155
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 37.9 bits (84), Expect = 0.005
Identities = 21/104 (20%), Positives = 51/104 (49%), Gaps = 9/104 (8%)
Frame = +3
Query: 261 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----F 413
+ D +++ +V + E++ + DG + ++G H+ ++ E Y S + +
Sbjct: 129 VKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYY 188
Query: 414 TRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPI 545
+LP++ + +++ L + GVL ++ PRT KN + + +
Sbjct: 189 NTSLSLPDDAKVEDIKAELKN-GVLNLVIPRTEKPKKNVQEISV 231
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 32.7 bits (71), Expect = 0.18
Identities = 20/81 (24%), Positives = 36/81 (44%)
Frame = +3
Query: 264 TSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENC 443
+ + D ++D+ EEI V+ D ++ + F R++ LPE+
Sbjct: 31 SQSPDSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESI 90
Query: 444 NPDTVESRLSSDGVLTVIAPR 506
+ + + DGVLTVI P+
Sbjct: 91 DMIGISAGYE-DGVLTVIVPK 110
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 29.1 bits (62), Expect = 2.2
Identities = 21/77 (27%), Positives = 29/77 (37%)
Frame = +3
Query: 318 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 497
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 498 APRTPAATKNERAVPIT 548
R KN +PI+
Sbjct: 108 DDRHSRMLKNVTELPIS 124
>At1g20410.1 68414.m02545 expressed protein
Length = 504
Score = 28.3 bits (60), Expect = 3.8
Identities = 17/53 (32%), Positives = 29/53 (54%)
Frame = -3
Query: 182 DGRQEIVRSKRQSEVLIAKAVRLVSQDEWQQRHFYFTLGCEISSDITVRSNSQ 24
D ++E+V+S S+ +A+ LV QD RH + + G E+S T+ N +
Sbjct: 111 DVKKELVKSDSSSDY-VARITDLVKQD----RHEFDSFGLEVSVPSTIMENER 158
>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
nearly identical to SH3 domain-containing protein 1
[Arabidopsis thaliana] GI:16974676; contains Pfam
profile PF00018: SH3 domain
Length = 439
Score = 27.9 bits (59), Expect = 5.0
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +2
Query: 140 LRTGAYSGRSPDGRHHANDVQRLLPAVEADGDGQ*RRR 253
+RT YS D RH N RL VEA RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 6.7
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +3
Query: 318 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 494
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 27.1 bits (57), Expect = 8.8
Identities = 16/50 (32%), Positives = 21/50 (42%)
Frame = +3
Query: 333 KTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDG 482
+ D Y EG EE ++ R Y LPE+ + VES DG
Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101
>At1g20740.1 68414.m02598 expressed protein ; expression supported
by MPSS
Length = 266
Score = 27.1 bits (57), Expect = 8.8
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -3
Query: 155 KRQSEVLIAKAVRLVSQDEWQQRHFYFTL 69
KR EVL + R + D+W F FT+
Sbjct: 237 KRTKEVLFFERYREIFNDDWDDHDFAFTI 265
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,651,504
Number of Sequences: 28952
Number of extensions: 225577
Number of successful extensions: 694
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 693
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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