Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmov11j21
(649 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse... 60 9e-10
At4g11550.1 68417.m01852 DC1 domain-containing protein contains ... 32 0.38
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui... 31 0.66
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 31 0.66
At5g14400.1 68418.m01682 cytochrome P450 family protein similar ... 30 1.5
At5g37060.1 68418.m04448 cation/hydrogen exchanger, putative (CH... 29 2.7
At5g63080.1 68418.m07917 transcription factor jumonji (jmjC) dom... 29 3.5
At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat... 29 3.5
At5g58460.1 68418.m07321 cation/hydrogen exchanger, putative (CH... 28 4.7
At4g11390.1 68417.m01837 DC1 domain-containing protein contains ... 28 4.7
>At5g27380.1 68418.m03269 glutathione synthetase (GSH2)
non-consensus AT donor splice site at exon 6, AC
acceptor splice site at exon 7; identical to
Swiss-Prot:P46416 glutathione synthetase, chloroplast
precursor (Glutathione synthase) (GSH synthetase)
(GSH-S) [Arabidopsis thaliana]
Length = 478
Score = 60.5 bits (140), Expect = 9e-10
Identities = 32/105 (30%), Positives = 59/105 (56%), Gaps = 5/105 (4%)
Frame = +3
Query: 273 LENKTLVNVIEKAKDWALMHGVGMRDKKHFNKDVI-----QIAPFILLPSPFPKTEFMKA 437
L+++ + ++ A W+ +HG+ + DK + + AP LLP+ FP+ + +A
Sbjct: 13 LDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGLMHAPIALLPTAFPEAYWKQA 72
Query: 438 VELQPVLNELMHKVAHDDEFLEQTLQNALQVDEFTANLYDIWLKV 572
+ P+ NEL+ +V+ D +FL+ +L +VD FT+ L DI K+
Sbjct: 73 CNVTPLFNELIDRVSLDGKFLQDSLSRTKKVDVFTSRLLDIHSKM 117
>At4g11550.1 68417.m01852 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 668
Score = 31.9 bits (69), Expect = 0.38
Identities = 13/32 (40%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = -1
Query: 586 MP-SFFTFNHMSYKFAVNSSTCRAFCKVCSKN 494
MP S T++H+SY+ N+S R FC+ C +
Sbjct: 595 MPGSTLTYSHISYEVVRNNSMSRPFCRACESH 626
>At4g38600.2 68417.m05463 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1794
Score = 31.1 bits (67), Expect = 0.66
Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
Frame = +3
Query: 363 NKDVIQIAPFILLPSPFPKT-------EFMKAVELQPVLNELMHKVAHDDEFLEQTLQNA 521
N+D++ +AP L P P+P T +F K +E +L +M K D L+ L A
Sbjct: 1467 NRDIV-LAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTA 1525
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 31.1 bits (67), Expect = 0.66
Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
Frame = +3
Query: 363 NKDVIQIAPFILLPSPFPKT-------EFMKAVELQPVLNELMHKVAHDDEFLEQTLQNA 521
N+D++ +AP L P P+P T +F K +E +L +M K D L+ L A
Sbjct: 1558 NRDIV-LAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTA 1616
>At5g14400.1 68418.m01682 cytochrome P450 family protein similar to
steroid 22-alpha-hydroxylase; DWF4; CYP90B1 (GI:2935342)
[Arabidopsis thaliana]
Length = 439
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = +3
Query: 390 FILLPSPFPKTEFMKAVELQPVLNELMHKVAHDDEFLEQTLQNALQVDEF 539
FI LP P P T + A++ + L+ + + + E E+ + NA++ ++F
Sbjct: 173 FISLPIPLPGTGYTNAIKARKRLSARVMGMIKEREREEEDMNNAIREEDF 222
>At5g37060.1 68418.m04448 cation/hydrogen exchanger, putative
(CHX24) similar to Na+/H+-exchanging protein NapA -
Enterococcus hirae, PIR:A42111; monovalent cation:proton
antiporter family 2 (CPA2) member, PMID:11500563
Length = 859
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/63 (23%), Positives = 29/63 (46%)
Frame = +3
Query: 453 VLNELMHKVAHDDEFLEQTLQNALQVDEFTANLYDIWLKVKNEGISHRYR*VCCVQTSCW 632
V NE +V++ + ++ + + N YD+W+ + EGI+ + S W
Sbjct: 745 VKNESNERVSYKEVVVKNGAETLAAIQAMNVNDYDLWITGRREGINPK----ILEGLSTW 800
Query: 633 SQD 641
S+D
Sbjct: 801 SED 803
>At5g63080.1 68418.m07917 transcription factor jumonji (jmjC)
domain-containing protein contains Pfam domain PF02373:
jmjC domain
Length = 462
Score = 28.7 bits (61), Expect = 3.5
Identities = 15/49 (30%), Positives = 29/49 (59%)
Frame = +2
Query: 20 AFTETNESCNSK*NLANSHFIFFS*FNLFDVLRKKNYNYLQQRINYCSI 166
A + N + N+ NL N F+F S F+L +++ ++Y+ + +N CS+
Sbjct: 306 AICQRNLAANTGMNL-NDFFLFMSRFSLGNMVLLQSYSDKHKNLNSCSL 353
>At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative strong
similarity to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 995
Score = 28.7 bits (61), Expect = 3.5
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = +3
Query: 336 VGMRDKKHFNKDVIQIAPFILLPSPFPKTEFMKAVELQPVLNEL 467
V +RD N++V Q+ + +LPS F K+ + E P+ NEL
Sbjct: 685 VYLRDVWPSNEEVAQVVQYSVLPSMF-KSSYETITEGNPLWNEL 727
>At5g58460.1 68418.m07321 cation/hydrogen exchanger, putative
(CHX25) similar to Na+/H+-exchanging protein slr1595,
Synechocystis sp., PIR:S74951; monovalent cation:proton
antiporter family 2 (CPA2) member, PMID:11500563
Length = 857
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/63 (23%), Positives = 29/63 (46%)
Frame = +3
Query: 453 VLNELMHKVAHDDEFLEQTLQNALQVDEFTANLYDIWLKVKNEGISHRYR*VCCVQTSCW 632
V NE +V++ + ++ + + N YD+W+ + EGI+ + S W
Sbjct: 743 VKNESNARVSYKEVVVKNGAETLAAIQAMNVNDYDLWITGRREGINPK----ILEGLSTW 798
Query: 633 SQD 641
S+D
Sbjct: 799 SED 801
>At4g11390.1 68417.m01837 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 514
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = -1
Query: 586 MP-SFFTFNHMSYKFAVNSSTCRAFCKVCSKN 494
MP S + SY+ +N+S R FC+VC +
Sbjct: 370 MPRSTIKYGSRSYEVVLNNSVSRPFCRVCESH 401
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,210,823
Number of Sequences: 28952
Number of extensions: 229783
Number of successful extensions: 641
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 635
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 641
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -