Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmov11i05
         (680 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...   144   1e-35
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch...    28   1.1  
SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces pomb...    28   1.4  
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces...    27   1.9  
SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |...    27   2.5  
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    27   3.3  
SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ...    27   3.3  
SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha...    27   3.3  
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote...    26   4.4  
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        26   5.8  
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    26   5.8  

>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score =  144 bits (349), Expect = 1e-35
 Identities = 72/140 (51%), Positives = 90/140 (64%)
 Frame = +3

Query: 213 RLSGIICTIGPASRNVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKL 392
           R + IICTIGP S NV  L K+ + GMN+ RMNFSHGS+EYH   I N R+A  +     
Sbjct: 26  RRTSIICTIGPKSNNVETLCKLRDAGMNIVRMNFSHGSYEYHQSVIDNARKASAT----- 80

Query: 393 GSPFSLAIALDTKGPEIRTGLLEGGGSAEVELKKGETIKLTTSSDYQEKGNADTIYVDYK 572
              F LAIALDTKGPEIRTGL  GG   +  +  G  +  TT   Y EK N   +Y+DYK
Sbjct: 81  NPLFPLAIALDTKGPEIRTGLTVGG--TDYPISSGHEMIFTTDDAYAEKCNDKVMYIDYK 138

Query: 573 NITNVVKPGNRIFIDDGLIS 632
           NIT V++PG  I++DDG++S
Sbjct: 139 NITKVIQPGRIIYVDDGILS 158


>SPBC25B2.07c |mug164||microtubule-associated
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 40/166 (24%), Positives = 66/166 (39%), Gaps = 3/166 (1%)
 Frame = +3

Query: 168 QHMCGLDIDSKSSYIRLSGI--ICTIGPASRNVAVLE-KMMETGMNVARMNFSHGSHEYH 338
           +H+  +   S SS  R+S    I  I  A  ++AV     +  G NV        +    
Sbjct: 148 KHVATITKPSTSSIARMSSNARIAAIPRAKSSMAVRSPSRLGNGPNVRSPKVGFNAKSDD 207

Query: 339 AETIRNCREAEKSYSAKLGSPFSLAIALDTKGPEIRTGLLEGGGSAEVELKKGETIKLTT 518
           +  +++    +K  ++   S  SL  +   + P  RT          V    G   K +T
Sbjct: 208 SPVVKSPGSNDKPSASPRISVRSLGNSSVVR-PPTRTSTTRPLSRVNVTNASGSISKNST 266

Query: 519 SSDYQEKGNADTIYVDYKNITNVVKPGNRIFIDDGLISIICQSVSA 656
           S   + K NA T  V   +    V+PG+RIF ++   +    +VSA
Sbjct: 267 SPS-KVKVNASTKIVRPVSAAQTVRPGSRIFRENSASNTQRPNVSA 311


>SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 334

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = -1

Query: 140 GLFNCTRESHGYVHRLIGLIYS*YFTVMLKVLN 42
           GLF C  +S  +  RL+ L Y  Y T  L++++
Sbjct: 118 GLFYCVCDSRHFTQRLVTLYYLNYLTKYLELMD 150


>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = -1

Query: 677 NGTRKSVSADRLTDDRDEAIIDEDSVSWLHDIR 579
           N TRKS+S  + TD      + +D  SWL  +R
Sbjct: 576 NKTRKSISHSKNTDKHVGNELPQDIPSWLRSLR 608


>SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 791

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 11/40 (27%), Positives = 22/40 (55%)
 Frame = +3

Query: 123 GAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGIICTIG 242
           G + + ++      L+ +   D+D+K +YI+LS    T+G
Sbjct: 15  GTLNESSIYKTYQILESLNPKDVDTKENYIKLSNTFSTLG 54


>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 608

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 23/89 (25%), Positives = 36/89 (40%), Gaps = 4/89 (4%)
 Frame = -2

Query: 469 PP--PSKSPVLISGPLVSNAMAKEKGEPSLAL*LFSASRQLRMVSAWYSWEPCEKFILAT 296
           PP  PS+ P + + P     +     EPS+   L      LR  +   + +P  K  L T
Sbjct: 430 PPKHPSRQPTVRATPSTGKQIEPPNDEPSIGNELLPLFESLRFENRKTTSKPKAKKTLVT 489

Query: 295 FIPVSIIFSSTATLRDAGPIVQI--IPER 215
            IP     +S   + D    + +  IPE+
Sbjct: 490 SIPTKPHQASVEKVYDLNSKINLKNIPEQ 518


>SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 736

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 15/38 (39%), Positives = 21/38 (55%)
 Frame = -2

Query: 499 SPFFSSTSAEPPPSKSPVLISGPLVSNAMAKEKGEPSL 386
           SP   ST+A  P  K+P   +  L+S   +KEK  PS+
Sbjct: 305 SPLHQSTTANHP-EKTPSRAAASLLSILDSKEKNTPSI 341


>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 370

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 10/24 (41%), Positives = 14/24 (58%)
 Frame = +2

Query: 551 YNLRGLQEHNECREARKPNLHR*W 622
           ++LRG   H  C EA+ PN+   W
Sbjct: 89  HDLRGSHLHTVCEEAKCPNIGECW 112


>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
            Mug36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1646

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 14/65 (21%), Positives = 29/65 (44%)
 Frame = +3

Query: 237  IGPASRNVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKLGSPFSLAI 416
            I P ++ +     ++++   V R+N +H S    ++   +      SY  +LG   S  I
Sbjct: 1194 ISPEAKTLLFQSIVIKSFSKVGRLNINHVSEPIDSDEFADYLSKSISYHLRLGKSKSKKI 1253

Query: 417  ALDTK 431
             ++ K
Sbjct: 1254 GIEIK 1258


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +2

Query: 185  RH*LQVFVHPSLRNNLYYWTGIP*CSCAR 271
            RH L++ V   L  N+Y WT +P C  +R
Sbjct: 2344 RHFLKLVV---LWKNIYVWTNVPDCDISR 2369


>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 10/17 (58%), Positives = 11/17 (64%)
 Frame = -2

Query: 499 SPFFSSTSAEPPPSKSP 449
           SP FSS+S  PPP   P
Sbjct: 231 SPIFSSSSTPPPPPPRP 247


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,565,925
Number of Sequences: 5004
Number of extensions: 49417
Number of successful extensions: 181
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)