BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov11e17 (555 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 32 0.049 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 25 5.7 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 32.3 bits (70), Expect = 0.049 Identities = 14/26 (53%), Positives = 20/26 (76%) Frame = +3 Query: 81 LDQQITKQGDLVRSLKTSKAEKSKID 158 ++++IT QG+LVRSLK+ A K ID Sbjct: 8 INERITSQGNLVRSLKSQGASKEDID 33 Score = 31.9 bits (69), Expect = 0.065 Identities = 14/35 (40%), Positives = 24/35 (68%) Frame = +3 Query: 264 SNDVTALDRDVTAQGDLVRSLKASKADKAKIDEAV 368 ++ + ++ +T+QG+LVRSLK+ A K ID+ V Sbjct: 2 ADSLVDINERITSQGNLVRSLKSQGASKEDIDKEV 36 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 25.4 bits (53), Expect = 5.7 Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 5/41 (12%) Frame = +1 Query: 205 PP--DKIGSQDPRLQQWPPPHRPMT---SPLSIVTSPRRET 312 PP DK G +D ++W P H P T S +S+++SP E+ Sbjct: 96 PPGDDKYGYEDAG-ERWLPVHSPETILISVISMLSSPNDES 135 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,465,621 Number of Sequences: 5004 Number of extensions: 20211 Number of successful extensions: 61 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 59 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 61 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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